F-box domain found in Arabidopsis thaliana Adagio proteins (AtADO1/2/3) and similar proteins
This subfamily contains Arabidopsis thaliana Adagio proteins, including AtADO1, AtADO2 and AtADO3. They are components of E3 ubiquitin ligase complexes that play central roles in blue light-dependent circadian cycles. They act as blue light photoreceptors, due to the presence of FMN, that mediate light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. AtADO1, also called clock-associated PAS protein ZTL, F-box only protein 2b (FBX2b), flavin-binding kelch repeat F-box protein 1-like protein 2 (FKL2), FKF1-like protein 2, LOV kelch protein 1 (LKP1), or protein ZEITLUPE, is a novel clock-associated PAS protein from Arabidopsis. Its circadian phase-specific degradation is mediated by the proteasome. AtADO2, also called F-box only protein 2c (FBX2c), flavin-binding kelch repeat F-box protein 1-like protein 1 (FKL1), FKF1-like protein 1, or LOV kelch protein 2 (LKP2), functions close to the circadian clock oscillator. AtADO3, also called F-box only protein 2a (FBX2a), or flavin-binding kelch repeat F-box protein 1 (FKF1), is essential for photoperiodic-specific light signaling and mediates cyclic degradation of a repressor of CONSTANS in Arabidopsis. The F-box domain has a role in mediating protein-protein interactions in a variety of contexts, such as polyubiquitination, transcription elongation, centromere binding and translational repression.
Feature 1:putative Skp1 binding site [polypeptide binding site]
Evidence:
Comment:based on the structure evidences that other F-box superfamily members interact with Skp1
Comment:F-box proteins participate in SCF (Skp1-Cul1-F-box protein) complexes that function as ubiquitin E3 ligases, where the role of the F-box protein is to recruit target substrates.
Jiyao W et al.(2023). "The conserved domain database in 2023.",