Conserved Protein Domain Family
deltaCoV_PLPro

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cd21734: deltaCoV_PLPro 
deltacoronavirus papain-like protease
This model represents the papain-like protease (PLPro) found in the non-structural protein 3 (Nsp3) region of deltacoronavirus, including Porcine deltacoronavirus, Bulbul coronavirus HKU11, and Common moorhen coronavirus HKU21. CoVs utilize a multi-subunit replication/transcription machinery. A set of non-structural proteins (Nsps) generated as cleavage products of the ORF1a and ORF1ab viral polyproteins assemble to facilitate viral replication and transcription. PLPro is a key enzyme in this process, making it a high value target for the development of anti-coronavirus therapeutics. PLPro, which belongs to the MEROPS peptidase C16 family, participates in the proteolytic processing of the N-terminal region of the replicase polyprotein; it can cleave Nsp1|Nsp2, Nsp2|Nsp3, and Nsp3|Nsp4 sites and its activity is dependent on zinc. Besides cleaving the polyproteins, PLPro also possesses a related enzymatic activity to promote virus replication: deubiquitinating (DUB) and de-ISGylating activities. Both, ubiquitin (Ub) and Ub-like interferon-stimulated gene product 15 (ISG15), are involved in preventing viral infection; coronaviruses utilize Ubl-conjugating pathways to counter the pro-inflammatory properties of Ubl-conjugated host proteins via the action of PLPro, which processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. The Nsp3 PLPro domain in many of these CoVs has also been shown to antagonize host innate immune induction of type I interferon by interacting with IRF3 and blocking its activation.
Statistics
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PSSM-Id: 409651
Aligned: 25 rows
Threshold Bit Score: 421.836
Created: 8-May-2020
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
Feature 1: Zn binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C CClick to see conserved feature residue pattern help
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
QDH76194     1090 MNKNHLQVWDaLNRTIVRTTTDYDQVTTKALTPQGVlEANLFDgEDFVQEPkPGQIYLEVTEevQNQAKELDLNlqQYCV 1169 Porcine delta...
YP_002308505 1134 LDSNHLQIWDaINRTVVRTTKDFDQVTTKALTPQGIlDANLFDgQEFVQDPkPGQVYLEVTDeiQQQAKELDLTlqQYVT 1213 Munia coronav...
ACJ12043     1049 KDVNHVQLWDaLNRTVVRTTKDFDQITTQALTPQGIlDANLFDgDTFVQEPvPDVVYLAVDDsvQEQAKELGLTlsQYCK 1128 Bulbul corona...
YP_002308496 1073 KDVNHLQLWDaLNRVVVRTTKDFDQITTQALTPQGVlDANLFDgDKFVQEPqPDCVYLAVDDvvQTKAKDLGLSlsQYCS 1152 Thrush corona...
BBC54841     1012 KDVKHLQLWDaLNRTVVRTTKDFDQVTTQALSPQGIlDANLFDgENYVQEPkPNEIYLAVDDkiQQHAKELGLSlkQYCN 1091 Pigeon corona...
BBC54831     1012 KDVKHLQLWDaLNRTVVRTTKDFDQVTTQALSPQGIlDANLFDgENYVQEPkPNEIYLAVDDkiQQHAKELGLSlkQYCN 1091 Houbara coron...
BBC54821     1012 KDVKHLQLWDaLNRTVVRTTKDFDQVTTQALSPQGIlDVNLFDgDNYVQEPkPNEIYLAVDDkiQQHAKELGLSlkQYCN 1091 Falcon corona...
YP_005352862 1053 LDKEHKNIWDaINATIVTYTYDMDQLYTGALSKDQIrQLNLFDgTDYVSEAvAGVTYLAVSDkvATSAKELDLTmsQYYK 1132 Night heron c...
YP_005352837 1055 KDVNHLQLWDaLNRIVVRTTKDFDQITTQALTPQGVlDANLFDgDVFVQEPsPNQVYLAVDDhiQQHARDLGLTlkQYCN 1134 White-eye cor...
YP_005352880  996 QDDRHLQAWDaINRVVVQTTKDFDQVTTQHLTPQGVlDANLYDgHDFVLEPkPNHVYLAVDDviQTAANDLDLTlsQYCM 1075 Common moorhe...
Feature 1                                                                                         
QDH76194     1170 YLKTCHHKWVVSRTNglMHLKQKDNNCFVSAGVNLFQNtAYQLRPAIDALYREYLNgNPNRFVAWIYAStNRRVGEMGCP 1249 Porcine delta...
YP_002308505 1214 YLKLCHANWPISRTNgvMHLKQHRNNCFVSAALNLFQNtRFQLRPAIDQLYKEFLNgNPSRLVAWVYAAtNHTIGEFGCP 1293 Munia coronav...
ACJ12043     1129 YLKACHHKWVTVRTNgiLHLKQKDNNCFVSAAINLFQAtHYKLRPAIDQLYQEYLNgNPCRFVAWIYASiDQTIGDMGCP 1208 Bulbul corona...
YP_002308496 1153 YLKMCHHKWATVRTNgiLHLKQKDNNCFISAAINLFQVtHYKLRPVIDELYQEYVNgNPNRLVAWIYASiNQNIGEMGCP 1232 Thrush corona...
BBC54841     1092 FLKYSLKKWTVQRTNgvVHLKQKDNNCFVSAALNLFQNtHYQPRFAIDELYQEYKNgNPSRLVAWIYASiDQEIGSMGCP 1171 Pigeon corona...
BBC54831     1092 FLKYSLKKWTVQRTNgvVHLKQKDNNCFVSAALNLFQNtHYQPRFAIDELYQEYKNgNPSRLVAWIYASiDQEIGSMGCP 1171 Houbara coron...
BBC54821     1092 FLKYSLKKWTVQRTNgvVHLKQKDNNCFVSAALNLFQNtHYQPRFAIDELYQEYRNgNPSRLVAWIYASiDQEIGSMGCP 1171 Falcon corona...
YP_005352862 1133 YLDYCSLQWKVKKTNf-THLVVVRNNCFITAVLDFLQNtHVTFRQPLDGLYKQYLNgNVEQLVAWCYAItNQTPGDTGDA 1211 Night heron c...
YP_005352837 1135 FLKYSHHKWSIQRINgvVHLKQKDNNCFVSSALNLFQNtHYRLTFAVDELYQEYKNgNPNRLVAWIYASvNHVIGDMGCP 1214 White-eye cor...
YP_005352880 1076 YLKNCQVRWTTKRVKglVHLQQQSNNCFVSSALNFYQNtHYKFRPVIDALYQEYLNgNPSRLVAWIYASiSQSIGELGCP 1155 Common moorhe...
Feature 1                             ##                        #  #                              
QDH76194     1250 QQVISLLVSnsDAAFSATTACCNTYFNHTGVIsvareYDPIQPKVYCMKCDVWTPFTPQSGkgaVAIGTSADEpt----- 1324 Porcine delta...
YP_002308505 1294 QEVIALFVNnaNSAFSATTVCCGTYFNHTGVIsvskdYDPLVPKVYCMKCDTWTPFNPVSGvgaIAVGTSDEEpt----- 1368 Munia coronav...
ACJ12043     1209 QQVTSLLVNnsNSKFSGTVHCCKTYFTHDGVVsnvreFDLLQPKVFCMRCDTWTTFSAENVegvIVLGNTPGKap----- 1283 Bulbul corona...
YP_002308496 1233 QQVTSLLVSnaNSKFFGTVHCCKTYFTHDGVIanvkdFDLLQPKVYCMRCESWTTFTPEQVegvVVLGNTIGPap----- 1307 Thrush corona...
BBC54841     1172 MQVISLLVNnsNCKFSGVTHCCNTYFTHDAIIantaeFDTMQPKVYCMRCDLWTPFTTEDTkgvVVLGNTSGPap----- 1246 Pigeon corona...
BBC54831     1172 MQVISLLVNnsNCKFSGVTHCCNTYFTHDAIIantaeFDTMQPKVYCMRCDLWTPFTTEDTkgvVVLGNTSGPap----- 1246 Houbara coron...
BBC54821     1172 MQVISLLVNnsNCKFSGVTHCCNTYFTHDAIIantaeFDTMQPKVYCMRCGLWTPFTTEDTkgvVVLGNTSGPap----- 1246 Falcon corona...
YP_005352862 1212 VAVLSALLKynTDTIIATTECCGSKVDLDGMIft-ttLLDFKPTVHCVKCDTFTGLSDLQVpgiVVACSNAIKpeeykts 1290 Night heron c...
YP_005352837 1215 QQVLSLLVNnaNCKFSGTANCCNTYFTHDGVIantnqSGPLEPEVFCMRCDKWTTFTPDNVdgvVVLGNVQGPat----- 1289 White-eye cor...
YP_005352880 1156 QQVLNLLVLnsNSKFSGVTHCCKRYFTHDGVVanveeFDLLQSKVYCVQCECWTTFDAEQTegvITVGRTSGPap----- 1230 Common moorhe...
Feature 1                                                                                         
QDH76194     1325 gPAIKFAAA---HCWYTngkKTVNGYDTKan-vVATYHRFDVPkpq-------------------------lvEDVVALP 1375 Porcine delta...
YP_002308505 1369 lPAIKYAQD---HCWYTngkKTINGYNAKak-vVATYHNFDVApap-------------------------rtETVSMPL 1419 Munia coronav...
ACJ12043     1284 tHAIQFGNS---HCWYTngkKSVNGYDIDsd-vVAIYHKFQTTpvr-------------------------niVVTQPIS 1334 Bulbul corona...
YP_002308496 1308 tHAICFGNA---HCWYTngrKAINGYDINan-vVVVYHKFQMMkt--------------------------qvVVSAPLS 1357 Thrush corona...
BBC54841     1247 tHAIMFDKD---HCWYTdgkKSINGYNTDan-vVAIYHKIQQVvnr-------------------------pvTVQDPLP 1297 Pigeon corona...
BBC54831     1247 tHAIMFDKD---HCWYTdgkKSINGYNTDan-vVAIYHKIQQVvnr-------------------------pvTVQDPLP 1297 Houbara coron...
BBC54821     1247 tHAIMFDKD---HCWYTdgkKSINGYNTDan-vVAIYHKIQQVvnr-------------------------pvTVQDPLP 1297 Falcon corona...
YP_005352862 1291 lPTISHNASgvgHWFAAl-nKGLDGSNLKgatiDVIYFKNQKPkp---------------------------vEDVLLDN 1342 Night heron c...
YP_005352837 1290 tHSIMFANS---HCWYTdgkKSINSYNVNan-vVAVYHKMQVArpmlvqpqatsqtltnqkvdvaknnkpfkpEVKVALP 1365 White-eye cor...
YP_005352880 1231 tHAIMFGNA---HCWYTdgrKSVNGYDIQad-vVAIYHKFNKVpeq-------------------------taMLAQVFE 1281 Common moorhe...
Feature 1                                            
QDH76194     1376 TKNDFEv--lNVEELp-----QDSVLh-LDPPPVQ 1402 Porcine deltacoronavirus
YP_002308505 1420 STNKYSv--lPTEDIp-----QSSPLl-LEPPPRE 1446 Munia coronavirus HKU13-3514
ACJ12043     1335 TSNTFSv--lPVQDIp-----KESILn-DDPSVVV 1361 Bulbul coronavirus HKU11-796
YP_002308496 1358 TSNNFDv--lKVQDIp-----EDSPLn-SDPPAVE 1384 Thrush coronavirus HKU12-600
BBC54841     1298 TSNSYEv--lKVEEPv-----SDDSTd-VETTEMV 1324 Pigeon coronavirus UAE-HKU29
BBC54831     1298 TSNSYEv--lKVEEPv-----SDDSTd-VETTEMV 1324 Houbara coronavirus UAE-HKU28
BBC54821     1298 TSNSYEv--lKVEEPv-----SDDSTd-VETTETV 1324 Falcon coronavirus UAE-HKU27
YP_005352862 1343 VVLTNRyqalAVEEIv-----QDTSEepVKQEETK 1372 Night heron coronavirus HKU19
YP_005352837 1366 VANTFDc--lKVEEIp-----TSSVLn-LDPKVKV 1392 White-eye coronavirus HKU16
YP_005352880 1282 TSNKYEv--lKVDPVinddqlQDVVVt-IQQPAVP 1313 Common moorhen coronavirus HKU21

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