3JC5,3JC6,6RAW,6XTX


Conserved Protein Domain Family
GINS_B_Psf1

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cd21696: GINS_B_Psf1 
beta-strand (B) domain of GINS complex protein Psf1
Psf1 (partner of Sld5 1) is a component of the GINS (named from the Japanese go-ichi-ni-san, meaning 5-1-2-3 for the Sld5, Psf1, Psf2, and Psf3 subunits) tetrameric protein complex, and is mainly expressed in highly proliferative tissues, such as blastocysts, adult bone marrow, and testis, in which the stem cell system is active. Psf1 has been reported to be a prognostic biomarker in breast cancer, prostate cancer, hepatocellular carcinoma, and non-small cell lung cancer (NSCLC) patients treated with surgery following preoperative chemotherapy or chemoradiotherapy. Loss of Psf1 causes embryonic lethality. GINS is a complex of four subunits (Sld5, Psf1, Psf2 and Psf3) and is involved in both the initiation and elongation stages of eukaryotic chromosome replication. Besides being essential for the maintenance of genomic integrity, GINS plays a central role in coordinating DNA replication with cell cycle checkpoints and is involved in cell growth. The eukaryotic GINS subunits Sld5, Psf1, Psf2 and Psf3 are homologous, and homologs are also found in archaea; the complex is not found in bacteria. The four subunits of the complex consist of two domains each, called the alpha-helical (A) and beta-strand (B) domains. The A and B domains of Sld5/Psf1 are permuted with respect to Psf1/Psf3. This model represents the B-domain of GINS subunit Psf1.
Statistics
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PSSM-Id: 412032
Aligned: 51 rows
Threshold Bit Score: 56.3775
Created: 21-Apr-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
tetramer
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:tetramer interface [polypeptide binding site]
Evidence:
  • Comment:the GINS complex contains four subunits: Sld5, Psf1, Psf2, and Psf3; it functions as a tight heterotetrameric complex in which major interactions are mediated through helix-helix interactions that amplify the helix-bundle-like structure of each individual subunit
  • Structure:3JC5: Saccharomyces cerevisiae GINS subunit Psf1 interface with other subunits in the heterotetramer; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1        #### #      #     # ##   # ####### #            # 
3JC5_A       161 AFIDVRVLKDAGE-IQTEYGVFn-LIKDSQFFVQQSDVERLIQQGYLQLI 208 baker's yeast
NP_178136    151 PYIKVRILDDIDE-GIVLSDKTtnFARHSMHFLKRTDAEPYIARGQMEEL 199 thale cress
EAY98271     148 PYIQVRVLEDIGE-VSLGDHSIs-LTKNSLHFLRRTDTEQFISQSLCENQ 195 Indian rice
XP_001563598 148 RQLEVRGRYQYEF-RSSVSGKTiiINAGDQIFLSFEEAEPLIQQGIVELV 196 Leishmania braziliensis MHOM/BR/75/M2904
XP_001697418 162 TFVNVRGLKDSGE-VILSYGRAt-VLVGTTISLPAEEAEPLLRDGTVEMV 209 Chlamydomonas reinhardtii
XP_002670485 103 DCVEVEVLKTSGS-RLLESGKTynLVQGSHLFIQLGDAEYLIQQGLVKLI 151 Naegleria gruberi strain NEG-M
EFN59792     165 PYVQVRVLRDYSD-VVFSSGKVs-LQRGKSHWLPRDEVHPLIMDGVLELV 212 Chlorella variabilis
CCC50352     229 RQLEVRGLVNHVF-VSSITGAVinLYKGKQILLCFEEAESLIQQGVVELV 277 Trypanosoma vivax Y486
CCA69621     160 LNVVVRVVRECGT-IQTELGPId-FQKGQRFMVRRADIEQLITQGFLEIV 207 Piriformospora indica DSM 11827
OEU06095     192 HMVQVRVLQNIGQiVLPDSGRSisMNKGSCLFLDRADVTDFLRQGLVQLY 241 Fragilariopsis cylindrus CCMP1102

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