4N0H


Conserved Protein Domain Family
GatF

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cd21422: GatF 
mitochondrial glutamyl-tRNA(Gln) amidotransferase subunit F
Glutamyl-tRNA(Gln) amidotransferase subunit F (GatF), also called Glu-AdT subunit F, is the connector subunit of yeast mitochondrial tRNA-dependent amidotransferase (AdT) that is also composed of the GatA and GatB subunits. GatA and GatB are well conserved among bacteria and eukaryota, but the GatF subunit is a fungi-specific ortholog of the GatC subunit found in all other known heterotrimeric AdTs. AdT allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). This model corresponds to the GatF subunit, which can be divided into two halves, the C-terminal GatC-like portion and the N-terminal appended domain (NTD).
Statistics
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PSSM-Id: 412058
Aligned: 19 rows
Threshold Bit Score: 115.124
Created: 30-Sep-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
trimer
Conserved site includes 58 residues -Click on image for an interactive view with Cn3D
Feature 1:trimer interface [polypeptide binding site]
Evidence:
  • Structure:4N0H: Saccharomyces cerevisiae GatF in complex with GatA and GatB; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1     #####  #  ## ## # # ### ##                   ##########           ## ### ### ## 
4N0H_F      9 IGKKFENMNQIRDYLSRpVWSVHEYLGIntkeekleppsAEAVKKLLRLSGLPLEga--dIKEIQMRLAKQLSFINKLHn 86  Saccharomyces cere...
A3LU55     22 IGSTLKTKEDIRNALNSpVWSLKELISSnekdmksvevsAKTINKVLKLSAFDTNitaeqEKSLVSALSAQMVFIKHLYe 101 Scheffersomyces st...
Q59X39     18 RFENLKTTEEIRNFLAEsTWSINELLKSptgss-qpevsPEIVKKMLKLSGLNDLk---dDQSVTKALNLQMMFINHLYd 93  Candida albicans S...
A5DGY1      2 SITRSLSKPAIRDLLNKpSWSITSLLHNpagie-tpkidEAVVKKMLRISGLQSTiskeeTTKIKKALETQMVFINHLYe 80  Meyerozyma guillie...
C5M7P6     38 LLDELKTSKDIEKFLNNsTWSIKELLQQpltntttkevsSDVVTKMLKLSGLSDSq---dIQNIRKSLNLQMMFINHLYd 114 Candida tropicalis...
Q6BXL8     27 LGPILENKSQIQDLVNKsEWNIVDIIKFsedevrnikidSRVITKMLRASGLKDSlsedqKKSLIRGLKLQMIFIKYLYe 106 Debaryomyces hanse...
C4Y3K5     17 VLPELKNAQEISQFLRKsTWNVHDLIPSkehit--nevdSRVVRKMLRLSGLDENlpepeLNRWAEMLNTHVAFINHVSd 94  Clavispora lusitan...
C4YKK2     18 RFENLKTTEEIRNFLAEsTWSINELLKSptgss-qpevsPEIVKKMLKLSGLNDLk---dDQSVTKALNLQMMFINHLYd 93  Candida albicans WO-1
B9WCK0     18 VFKNLRTSEEIRSFLAEsTWSISELLQPptgss-qtevsPEIVKKMLKLSGLNDLk---hDKSVTKALNLQMMLINHLYd 93  Candida dubliniens...
C4R8Q1     13 VGQKFQSLEQIKEYLKKpTWDTKTLLKGsqks---leidTPKLHRLLQLSGLSTDldagkERQLLNDLNAQLTMMNKLQe 89  Komagataella phaff...
Feature 1                             #    #####  #  ##  ####   #### ## # #
4N0H_F     87 ipveg-----ekhtkEYDARLVQRNt---KQLNYTKLLEGISHQKQDa--ELGEVSGSWKA 137 Saccharomyces cerevisiae S288C
A3LU55    102 ndeksd---svveenDTHFRLIASDhnpgEPLTLKQLLAQIEELPEKvdpAKGETQGSFNV 159 Scheffersomyces stipitis CBS 6054
Q59X39     94 ndhetvtpspkrnenNGIFRLLASDhlpqRPLELNNLLKQINELKPDp--SKGEVDFTISD 152 Candida albicans SC5314
A5DGY1     81 ddnva----nnnstnNDHFRLLPGDhrqqEPLNLQQIEESISSLKPSa--EKGEIGNFTIG 135 Meyerozyma guilliermondii ATCC 6260
C5M7P6    115 ksgnna--ekkvndnNCMFRLLASDhipqRPLDLDTLMDEINKLEPSe--EKGEIGFGIKD 171 Candida tropicalis MYA-3404
Q6BXL8    107 gdeaef--hkieesnDDVFRLILSDhkapKPITLKSLLSSIENLENEvdaEKGEIKSSLDI 165 Debaryomyces hansenii CBS767
C4Y3K5     95 lhsst-----kgeigSSVFRLLASDhkpeSPLTLKELLRQVDEISDHvsdQRGERGFDTSE 150 Clavispora lusitaniae ATCC 42720
C4YKK2     94 ndhetvtpspkrnenNGIFRLLASDhlpqRPLELNDLLKQINELKPDp--SKGEVDFTISD 152 Candida albicans WO-1
B9WCK0     94 neqdtvtpsrerndnNGIFRLLASDhlpqQPWDLDDLLKQINELKPDp--SKGEVDFTISD 152 Candida dubliniensis CD36
C4R8Q1     90 i-------------eCKEFDLIETQh---SPLKFQDIRNATGELRQSs--KKGETGQWNPL 132 Komagataella phaffii GS115

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