Conserved Protein Domain Family
ZBD_rv_SF1_Hel-like
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cd21404: ZBD_rv_SF1_Hel-like 
Cys/His rich zinc-binding domain (CH/ZBD) of ronidovirus SF1 helicase and related proteins
Helicases catalyze NTP-dependent unwinding of nucleic acid duplexes into single strands and are classified based on the arrangement of conserved motifs into six superfamilies. This ronidovirus group includes Gill-associated virus (GAV) replication helicase encoded on ORF1 and belongs to helicase superfamily 1 (SF1). The CH/ZBD has 3 zinc-finger (ZnF1-3) motifs. Members of this group belong to a family of nindoviral replication helicases which include includes Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) non-structural protein 13 (SARS-Nsp13), a component of the viral RNA synthesis replication and transcription complex (RTC). The SARS-Nsp13 CH/ZBD is indispensable for helicase activity and interacts with SARS-Nsp12, the RNA-dependent RNA polymerase. SARS-Nsp12 can enhance the helicase activity of SARS-Nsp13.
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Statistics
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PSSM-Id: 394811
Aligned: 3 rows
Threshold Bit Score: 200.297
Created: 12-Mar-2020
Updated: 17-Oct-2022
Structure
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Aligned Rows:
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Feature 1: Zn binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C [CH]Click to see conserved feature residue pattern help
Evidence:
  • Comment:based on SARS-Nsp13 and EAV-Nsp10 with structures
  • Comment:named the Zn1 binding site in SARS-Nsp13 and EAV-Nsp10
  • Comment:zinc binding is essential for the structure and function of coronavirus SARS-Nsp13 and EAV-Nsp10 helicase
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #  #                    #    #                                                
YP_001661452 5241 DICFACGGHgqFECYTCasngYPFTFCngsTCLRTHFTECEHYSYSLAsRKRYIIQCaDCEEMDIRKMYVNnDFRCARHL 5320 Gill-associat...
ACS44772     5256 DVCFACGGYgqFECYTCasagYPFTFCngaTCLRTHITDCEHYSYSLAaRKRYIIQCsECEEMDIRKMYVDnDFRCAQHL 5335 Yellow head v...
YP_009666324 5256 DVCFACGGYgqFECYTCasagYPFTFCngaTCLRTHITDCEHYSYSLAaRKRYIIQCsECEEMDIRKMYVDnDFRCAQHL 5335 Yellow head v...

Feature 1                                  
YP_001661452 5321 HtegYSKITDDKNNVTIfRTAATRS 5345 Gill-associated virus
ACS44772     5336 DkegYSKITDDKNNVTIfRTNATRS 5360 Yellow head virus
YP_009666324 5336 DkegYSKITDDKNNVTIfRTNATRS 5360 Yellow head virus

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