Conserved Protein Domain Family
ZBD_mv_SF1_Hel-like
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cd21402: ZBD_mv_SF1_Hel-like 
Cys/His rich zinc-binding domain (CH/ZBD) of mesnidovirus SF1 helicase and related proteins
Helicases catalyze NTP-dependent unwinding of nucleic acid duplexes into single strands and are classified based on the arrangement of conserved motifs into six superfamilies. This mesnidovirus group includes the Bontag Baru virus (BBaV) replication helicase encoded on ORF1b and belongs to helicase superfamily 1 (SF1). The CH/ZBD has 3 zinc-finger (ZnF1-3) motifs. Members of this group belong to a family of nindoviral replication helicases which include includes Severe Acute Respiratory Syndrome coronavirus (SARS-CoV) non-structural protein 13 (SARS-Nsp13), a component of the viral RNA synthesis replication and transcription complex (RTC). The SARS-Nsp13 CH/ZBD is indispensable for helicase activity and interacts with SARS-Nsp12, the RNA-dependent RNA polymerase. SARS-Nsp12 can enhance the helicase activity of SARS-Nsp13.
Links
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Statistics
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PSSM-Id: 394809
Aligned: 9 rows
Threshold Bit Score: 193.112
Created: 10-Mar-2020
Updated: 25-Oct-2021
Structure
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Aligned Rows:
Download Cn3D
 
Feature 1: Zn binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C [CH]Click to see conserved feature residue pattern help
Evidence:
  • Comment:based on SARS-Nsp13 and EAV-Nsp10 with structures
  • Comment:named the Zn1 binding site in SARS-Nsp13 and EAV-Nsp10
  • Comment:zinc binding is essential for the structure and function of coronavirus SARS-Nsp13 and EAV-Nsp10 helicase
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #  #               #         #                                                
AGL73177     1089 YNCYVCGENayLTCATCERAFCNSADTNHGSHIEQHLQYSGHTCLYLNCKTVKCqHCFTNDINLLYTTg-rEHFCVsHKP 1167 Bontang virus
YP_009666293 3607 YNCYVCGENahITCALCERVFCNSKDSTHGSHIEQHLQYSGHNSLYLNCKTVKCrHCYVTDINNLYTAgkdMYYCLvHKP 3686 Kadiweu virus
YP_009026378 3588 YNCYVCGENayLTCATCERAFCNSADTNHGSHIEQHLQYSGHNCLYLNCKTVKCsHCYTTDIHLLYTNg-rDHYCEsHKP 3666 Casuarina virus
YP_009666297 3561 YNCYVCGGTayLTCATCERVFCNSIDKHHGSHIEQHLQYSGHDRLYLNCKLVRCkHCYITDINKLYTSgrdLYFCNsHKP 3640 Ofaie virus
YP_007697636 3584 YNCYVCGENayITCATCEKSFCNSKDTIHGSHIEQHLQYSGHDCLYLNCKTIKCrHCFAIDINKLYTSgkdMYFCEtHKP 3663 Meno virus
AII17278     1049 YNCYVCGENayLTCATCERAFCNSADTNHGSHMEQHLQYSGHTCLYLNCKTVKCqHCFTTDINLLYTTg-rDHYCEsHKP 1127 Nam Dinh virus
AGL73186     1050 YNCYVCGENayLTCATCERAFCNSADTNHGSHMEQHLQYSGHTCLYLNCKTVKCqHCFTTDINLLYTTg-rDHYCEsHKP 1128 Alphamesonivi...
YP_009551696 3872 TFCYVCGGNayITCAICYKSFCNSIDNNHGSHLEQHYYLSHHERYTVNSRAISCsHCYENDISKLYRVtpeKYACEqHKP 3951 Yichang virus
ABG02430     3612 YNCYVCGENayLTCATCERAFCNSADTNHGSHMEQHLQYSGHTCLYLNCKTVKCqHCFTMDINLLYTTg-rDHYCEsHKP 3690 Nam Dinh virus

Feature 1                                        
AGL73177     1168 KNAARILnsnanskLPPLLYLCVTNTRQVTF 1198 Bontang virus
YP_009666293 3687 TQSVKLLnnnvnekLPPNLYLCITDSRPVPF 3717 Kadiweu virus
YP_009026378 3667 KNAVRILnnnvnanLPPLLYLCVTDARRIPF 3697 Casuarina virus
YP_009666297 3641 PNAVSLItnnedklLPPRLFLCVIEPKLVSF 3671 Ofaie virus
YP_007697636 3664 KHAIRLVnnnvasnLPPLLYLCVTEAKSVSF 3694 Meno virus
AII17278     1128 KNAVRILnyndntkLPPRLYLCVTDTKRVTF 1158 Nam Dinh virus
AGL73186     1129 KNAVRILnynantkLPPLLYLCVTDTKRVTF 1159 Alphamesonivirus 1 Ngewotan
YP_009551696 3952 ANASALVtpt-ntaTPKHINLCVVRSPATNI 3981 Yichang virus
ABG02430     3691 KNAVRILnynantkLPPLLYLCVTDTKRVTF 3721 Nam Dinh virus

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