2PJW


Conserved Protein Domain Family
GAT_Vps27

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cd21385: GAT_Vps27 
non-canonical GAT domain found in fungal vacuolar protein sorting-associated protein 27 (Vps27) and similar proteins
Vps27, also called Golgi retention defective protein 11 (GRD11), is a component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB), and recruits ESCRT-I to the MVB outer membrane. It controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Members of this family contain a non-canonical GAT (GGA and Tom1) domain consisting of two helices. A canonical GAT domain is a monomeric three-helix bundle that bind to ubiquitin. Vps27, together with another GAT domain-containing protein Hse1, forms a Vps27/Hse1 core complex that consists of two intertwined non-canonical GAT domains, each consisting of two helices from one subunit, and one from the other subunit. The two GAT domains are connected by a two-stranded coiled-coil. The Vps27/Hse1 complex, an intertwined GAT heterodimer, is a scaffold for binding of ubiquitinated cargo proteins and coordinating ubiquitination and deubiquitination reactions that regulate sorting.
Statistics
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PSSM-Id: 410590
Aligned: 13 rows
Threshold Bit Score: 105.863
Created: 6-Feb-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 41 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Comment:The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting.
  • Structure:2PJW; Saccharomyces cerevisiae Vps27 in complex with Hse1, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #    #  ## ##  ## ## #      #  ##    #     ##  #  ##  #  ## ## ##  ## ###### ##
2PJW_V         2 DLSDEEKDSIYXFASLVEKXKSRp----lNEILEDSKLQNLAQRVFASKARLNYALNDKAQKYNTLIEXNGKISEIXNIY 77  baker's yeast
P0CS27       335 DLSPRENETILTFSNTMDQMAAYg----eRDLRRFPHAHVLAEQANTVGGRLRRNVEEKSTKQQMLMEMQDKLSQAVNLY 410 Cryptococcus ne...
XP_018224345 385 ELTASEIENINTFCMLIQGLQMAp----iGSILRDHYVQELHDSVVKLKPKLIRSLGNTIKKLIDIHSKLSTVMKYYDKL 460 Pneumocystis ca...
A3LX75       382 SLTEEEEADINKFITLMYQVKNDqn--isYARLHDEKLTDLHTKVCPLRSKVTKNLIYTVERQKAFTELNNKIAAITRLY 459 Scheffersomyces...
Q6FQJ1       337 ELTPADEEDIYLFASLVERMKTRp----pSEILDDPQLQQLAQKVFASKPRLGNTLNSKIQKYNTLVDMNGKISHIMNTY 412 [Candida] glabr...
P40343       349 DLSDEEKDSIYMFASLVEKMKSRp----lNEILEDSKLQNLAQRVFASKARLNYALNDKAQKYNTLIEMNGKISEIMNIY 424 Saccharomyces c...
Q4P7Q1       405 DLAPQDVDNILTFSQTVQAQQAHnqrtgmVGAPLPASVQAMYEKASSARPRMARNLEEGHRRHNVLVSMHDKLTEAVHLY 484 Ustilago maydis...
Q6BSD6       397 DLSQQEEESINLFITLMNNIKSDps--kqANILYDSNLSELHGKIIQLKPKLNKSLRASIEKYETCLELNNKISTISRLY 474 Debaryomyces ha...
O13821       368 LISTADADNITLYATLVQKLKKLp----pGSIFTEYQLQELHENMGVMRTRMMRSLGETMSKYNGLIQALQKLQTCMRLN 443 Schizosaccharom...
RPD70819     380 DLEPLEEDAILTFNQTVEQVQAQg----gRDLSRYPAVTELFDKANALRPKLAMGLNDTSRKEQMLVDMHEKLSQAVKLY 455 Lentinus tigrin...
Q755J9       359 TLTNQEEDDIYLFANLVEKMKGQp----mNAVLEDTQLQMLYQKVLGTRPRLNHSLNDTFHKYNTLVDMNAKISDIMSIY 434 Eremothecium go...
Q7RZJ2       368 ELTPVEAENINLFATLVDRLQTQp----pGTILREPQIQELYDSIGALRPKLARTYGETMSKHDTLLDLHAKLSTVVRYY 443 Neurospora cras...
Q6CFT4       339 DLTATEESNINLLSLLVERLKGEp----qGTILREPKIQELYDTVGNLRPKLARTMGETISKYDSLVDMHAKMTTVIRYY 414 Yarrowia lipoly...
Feature 1          ## ## 
2PJW_V        78 DRLLEQQL 85  baker's yeast
P0CS27       411 GQILDGQQ 418 Cryptococcus neoformans var. neoformans B-3501A
XP_018224345 461 LEDRLSTA 468 Pneumocystis carinii B80
A3LX75       460 EEHLDSKL 467 Scheffersomyces stipitis CBS 6054
Q6FQJ1       413 DNLLEQEL 420 [Candida] glabrata CBS 138
P40343       425 DRLLEQQL 432 Saccharomyces cerevisiae S288C
Q4P7Q1       485 DRLLDAQM 492 Ustilago maydis 521
Q6BSD6       475 DQFLEAKL 482 Debaryomyces hansenii CBS767
O13821       444 DALIEQRL 451 Schizosaccharomyces pombe 972h-
RPD70819     456 DKLLTEQL 463 Lentinus tigrinus ALCF2SS1-7
Q755J9       435 DTMLERQL 442 Eremothecium gossypii ATCC 10895
Q7RZJ2       444 DRMLEERL 451 Neurospora crassa OR74A
Q6CFT4       415 DALLEEKL 422 Yarrowia lipolytica CLIB122

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