2PJW,2PJW,3F1I,3F1I


Conserved Protein Domain Family
GAT_STAM_Vps27-like

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cd21384: GAT_STAM_Vps27-like 
non-canonical GAT domain found in metazoan signal transducing adapter molecules (STAMs), fungal vacuolar protein sorting-associated protein 27 (Vps27), and similar proteins
This family includes several components of the ESCRT-0 complex, including STAMs, hepatocyte growth factor-regulated tyrosine kinase substrate (Hrs), as well as vacuolar protein sorting-associated protein 27 (Vps27) and class E vacuolar protein-sorting machinery protein Hse1 from fungi. The ESCRT-0 complex binds ubiquitin and acts as a sorting machinery that recognizes ubiquitinated receptors and transfers them for further sequential lysosomal sorting/trafficking processes. Members in this family contain a non-canonical GAT (GGA and Tom1) domain consisting of two helices. By contrast, a canonical GAT domain is a monomeric three-helix bundle that bind to ubiquitin. Hrs together with STAM forms a Hrs/STAM core complex. Vps27, together with Hse1, forms a Vps27/Hse1 core complex. Those complexes consist of two intertwined non-canonical GAT domains, each consisting of two helices from one subunit, and one from the other subunit. The intertwined GAT heterodimer acts as a scaffold for binding of ubiquitinated cargo proteins and coordinating ubiquitination and deubiquitination reactions that regulate sorting.
Statistics
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PSSM-Id: 410589
Aligned: 4 rows
Threshold Bit Score: 86.0607
Created: 17-Aug-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 40 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Comment:The STAM/Hrs complex and Vps27/Hse1 complex are GAT domain-based scaffolds for ubiquitin-dependent sorting.
  • Structure:3F1I; Homo sapiens STAM1 in complex with Hrs, contacts at 4A
    View structure with Cn3D
  • Structure:2PJW; Saccharomyces cerevisiae Vps27 in complex with Hse1, contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1     # #  ## ##  #  #              #   #  ##  ## ###### ### ## ######## ### ##  ##  
2PJW_V     5 DEEKDSIYXFASLVEKXKsrpln-eileDSKLQNLAQRVFASKARLNYALNDKAQKYNTLIEXNGKISEIXNIYDRLLEQ 83  baker's yeast
2PJW_H     6 FSQKTTIDQLHNSLNAASktgnsnevlqDPHIGDXYGSVTPLRPQVTRXLGKYAKEKEDXLSLRQVLANAERSYNQLXDR 85  baker's yeast
3F1I_S     1 FIDEDKMDQLLQMLQSTDpsdd---qpdLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNE 77  human
3F1I_H     7 KALQNAVTTFVNRMKSNHmrgr--sitnDSAVLSLFQSINGMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSAL 84  human

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