1WR6,1WRD


Conserved Protein Domain Family
GAT_GGA_Tom1-like

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cd21383: GAT_GGA_Tom1-like 
canonical GAT domain found in eukaryotic ADP-ribosylation factor (Arf)-binding proteins (GGAs), metazoan myb protein 1 (Tom1)-like proteins, and similar proteins
This model represents the canonical GAT (GGA and Tom1) domain found in GGAs from eukaryotes, Tom1-like proteins from metazoa, and LAS seventeen-binding protein 5 (Lsb5p)-like proteins from fungi. The canonical GAT domain is a monomeric three-helix bundle that binds ubiquitin. GGAs, also called Golgi-localized gamma-ear-containing Arf-binding proteins, belong to a family of ubiquitously expressed, monomeric, motif-binding cargo/clathrin adaptor proteins that regulate clathrin-mediated trafficking of cargo proteins from the trans-Golgi network (TGN) to endosomes. GGAs play important roles in ubiquitin-dependent sorting of cargo proteins both in biosynthetic and endocytic pathways. Tom1 and its related proteins, Tom1L1 and Tom1L2, form a protein family sharing an N-terminal VHS-domain followed by a GAT domain. Tom1 family proteins bind to ubiquitin, ubiquitinated proteins, and Toll-interacting protein (Tollip) through its GAT domain. They do not associate with either Arf GTPases through its GAT domain nor with acidic cluster-dileucine sequences through its VHS domain. In addition, Tom1 family proteins recruit clathrin onto endosomes through their C-terminal region. The C-terminal clathrin-binding region of Tom1 and Tom1L2 are similar to each other, but distinguishable from Tom1L1.
Statistics
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PSSM-Id: 410588
Aligned: 31 rows
Threshold Bit Score: 54.5773
Created: 4-Mar-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
ubiquitin
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:ubiquitin binding site [polypeptide binding site]
Evidence:
  • Structure:1WR6; Homo sapiens ADP-ribosylation factor-binding protein GGA3 binds ubiquitin through its GAT domain, contacts at 4A.
    View structure with Cn3D
  • Structure:1WRD: Human TOM1 GAT domain binds ubiquitin; contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           ##  ## ##  ##  #            #  ##                                             
1WR6_A          9 TLEEVNNNVRLLSEXLLHysqedssdgdrELXKELFDQCENKRRTLFKLASETed------------------------- 63   human
P25369        219 ISDALASAVSLQNSLIGLpkgkf--stddEEATSKFIQARAIRRKVLRYLQLVte------------------------- 271  Saccharomyces...
XP_005825717  127 EMKMARGSLELLLDCLAAadp------deEILSQVARNATEYRKKLELSIQRTee------------------------- 175  Guillardia th...
EMD35067      390 ECSLGKGNAQLLSEALAFakped--lkhkDIIREFYARCRASQELISAQIPWAfagaersreaaarrevkprhrqqqtep 467  Ceriporiopsis...
EGF98237      221 EAEVARSNARLLIEALAYttpae--mennELIQEFHSKCLHHQNQLLDDIPWAtaqaeqsrlyassittshplpatn--- 295  Melampsora la...
EIW68575      784 ECAAAKESARVLGEALVYtrpee--lehkPVVREFYTKCFHAHESLTNQMDWAqaeasrsreg----------------- 844  Tremella mese...
CCM03840       34 LCKAARGNADLLHEALVNsegpr--dlkgDLVQEFLRKCRQSQDVVQSHVPMVkaevdqaril----------------- 94   Fibroporia ra...
Q7SDP6        254 RLEEARTSAKLLEQVVACtpphe--ilsnDLIKEFADRCTSASRSIQEYMSATdpa------------------------ 307  Neurospora cr...
Q5BFA3        207 RISEAKTTAKLLIQFVQStppse--mltnDLIQEFSARSRRAQRAISNYIHATnpa------------------------ 260  Emericella ni...
RPD79792       80 ACKVGRGNAELLHEVLVYakpq---elknEVTKELLERARASIDLIGSQIPWAtteaersrra----------------- 139  Lentinus tigr...
Feature 1                                                            
1WR6_A         64 --------------------------ndNSLGDILQASDNLSRVINSYKTI 88   human
P25369        272 ---------------------------gEFLGSLIHANDELVAALTAYDDR 295  Saccharomyces cerevisiae S288c
XP_005825717  176 ---------------------------eARLQALLDLHSSLVEALALYQQK 199  Guillardia theta CCMP2712
EMD35067      468 rasletdhaqllqepvssqesvaltieeELLAALLAANEELLEALRVYDDL 518  Ceriporiopsis subvermispora B
EGF98237      296 -----pyapllngvgtppdpssgltkeeELLNVLLLANSDLVDGFRQYDEM 341  Melampsora larici-populina 98AG31
EIW68575      845 ------------------npdqgstseeRALASLFEAHGLLSEALRQYDDL 877  Tremella mesenterica DSM 1558
CCM03840       95 ------------------smgqehssmtRYLKELYKVNDGLSIVLQLYDAL 127  Fibroporia radiculosa
Q7SDP6        308 -------------------------pdnDTMESLIDTNEQLQQSLNHHQRA 333  Neurospora crassa
Q5BFA3        261 -------------------------pdeDTLLTLIETNDELSVALSKHQRA 286  Emericella nidulans
RPD79792      140 ------------------aytasettqeQLLAALLGAHEQLTECIKMYDDL 172  Lentinus tigrinus ALCF2SS1-7

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