Conserved Protein Domain Family
SPASM_MftC-like
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cd21123: SPASM_MftC-like 
Iron-sulfur cluster-binding SPASM domain of mycofactocin radical SAM maturase MftC and similar proteins
This group is composed of Mycobacterium tuberculosis putative mycofactocin radical SAM maturase MftC and similar proteins. MftC is a radical S-adenosylmethionine (SAM) enzyme that may function to modify mycofactocin, a conserved polypeptide that might serve as an electron carrier. Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. Radical SAM enzymes with a C-terminal SPASM domain contain at least one other iron-sulfur cluster. This group appears to contain one auxillary Fe-S cluster that is similar to the second auxillary 4Fe-4S cluster (AuxII) of Clostridium perfringens anaerobic sulfatase-maturating enzyme (anSME).
Links
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Statistics
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PSSM-Id: 410614
Aligned: 32 rows
Threshold Bit Score: 89.624
Created: 22-Jan-2020
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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putative Fe-S
Feature 1: putative Fe-S cluster binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C CClick to see conserved feature residue pattern help
Evidence:
  • Comment:based on the structure of Clostridium perfringens anaerobic sulfatase-maturating enzyme with bound auxillary 4Fe-4S cluster remote to the radical SAM site
  • Comment:likely binds a 4Fe-4S cluster at this site
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                               #  #     
P9WJ78       248 LNMCGAGrVVCLIDPvGDVYACPfa-ihDHFLAGNVLsdggFQNVWKNss---lFRELREPQs-----aGACGSCGHYDS 318 Mycobacterium t...
WP_010878621 278 RGGDSSGiRLANIDHqGNVHPNQf---wWDYTVGNVLer-sFEEIWMGed--elLKKLREKTky---lrDKCGKCHFKEI 348 Archaeoglobus f...
WP_010902854 290 NGGDPTGeRIADVDYqGNVHMTQf---wQGYSLGNVRdr-sFGDIWSDdtn-plLAGLRDRDdr---ltGRCASCQYRSI 361 Halobacterium s...
NP_051702    277 EVGDVAGiSELCISSdGEVYPSVllvgeKTMSCGNLReh-sLQRIWQEss---cLMALRELKlfd--lnGNCTKCGVREV 350 Deinococcus rad...
WP_014425380 288 NGGNRSGiAFGEVDPlGYVHPDQf---tQHHTFGNVRer-kFGDIWSDtsvspiLAGLKDRKpl---lkGRCAKCKFLDN 360 Selenomonas rum...
AAM06408     290 NQGNSSGiGIGCVSWdGSVHADQf---wRHYSFGNVRer-pFSEIWTDqsd-elMAGLKHRKpliqanaDRCAKCKWFDV 364 Methanosarcina ...
NP_215207    248 LNMCGAGrVVCLIDPvGDVYACPfa-ihDHFLAGNVLsdggFQNVWKNss---lFRELREPQs-----aGACGSCGHYDS 318 Mycobacterium t...
Q5WKR5       289 TNQEKCGrTIAYINSaGDVYPCSncmsnDDYKAGNLRdh-sFKDIWEQgf--deFRSITFDEf------AGCQSCAVYQE 359 Bacillus clausi...
WP_011994030 227 GEECTAGrTHASILVdGNVVPCGhfrynPEFYMGNVRne-rFKVIWEK------YNNQILLP-------NVCAKCSLRNV 292 Fervidobacteriu...
WP_005867070 264 IFQCNAGiNTASVLAdGAISGCPsi--rANFHQGNIYkd-dFIDIWNNe-----FKPYRNRQwa---kkGECADCKMFRY 332 Parabacteroides...

Feature 1        #                      # 
P9WJ78       319 CRGg-CMAAKFFtglplDGPDPECV 342 Mycobacterium tuberculosis CDC1551
WP_010878621 349 CGG--FRVRAYRyg-dlWGADPSCY 370 Archaeoglobus fulgidus
WP_010902854 362 CRGg-SRLRALSag-gpFDPDPKCY 384 Halobacterium salinarum
NP_051702    351 CGGg-SRSRAFSregdfRDMDYVCP 374 Deinococcus radiodurans R1
WP_014425380 361 CNGn-FRTRAEAvtgdfWESDPSCY 384 Selenomonas ruminantium
AAM06408     365 CNGn-FRVRAEAvygnvWADDPACY 388 Methanosarcina acetivorans C2A
NP_215207    319 CRGg-CMAAKFFtglplDGPDPECV 342 Mycobacterium tuberculosis H37Rv
Q5WKR5       360 GIWcqFRCPPLAkn--vSGDKLGCG 382 Bacillus clausii KSM-K16
WP_011994030 293 SCKsgCLAYAYLne--sKVDDIHCK 315 Fervidobacterium nodosum
WP_005867070 333 CEG--NGMHLYDd----EGNLLVCH 351 Parabacteroides distasonis

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