Conserved Protein Domain Family
SPASM_Cmo-like

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cd21121: SPASM_Cmo-like 
Iron-sulfur cluster-binding SPASM domain of tungsten-containing aldehyde ferredoxin oxidoreductase cofactor-modifying protein and similar proteins
This group is composed of Pyrococcus furiosus tungsten-containing aldehyde ferredoxin oxidoreductase (AOR; EC 1.2.7.5) cofactor-modifying protein, encoded by the cmo gene, and similar proteins. AOR cofactor-modifying protein is involved in the biosynthesis of a molybdopterin-based tungsten cofactor. Members of this group are radical S-adenosylmethionine (SAM) enzymes with a SPASM domain. Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. Radical SAM enzymes with a C-terminal SPASM domain contain at least one other iron-sulfur cluster. This group appears to contain one auxillary Fe-S cluster, similar to the auxillary 4Fe-4S cluster in Bacillus circulans butirosin biosynthetic enzyme BtrN.
Statistics
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PSSM-Id: 410612
Aligned: 29 rows
Threshold Bit Score: 79.6481
Created: 22-Jan-2020
Updated: 25-Oct-2021
Structure
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Aligned Rows:
 
putative Fe-S
Feature 1: putative Fe-S cluster binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C CClick to see conserved feature residue pattern help
Evidence:
  • Comment:based on the structure of Bacillus circulans butirosin biosynthetic enzyme BtrN with bound auxillary 4Fe-4S cluster
  • Comment:likely binds a 4Fe-4S cluster at this site

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #                  #                                                          
Q51741       231 RVCDFDenKVAVIRWdGEVVPCYRFlhtyreyi-----fgrekkvnTYSFGNVReksLADIWTSerYTWFRFITKny--- 302 Pyrococcus furi...
ABW02617     206 TECPERptETIFINVdGYVSPCVFLnlptrsnyiprcfmgkcfkvkKVIFGNLNndrIMNIWNSeaYREFRFKFIrrhqd 285 Caldivirga maqu...
WP_012546361 246 EICSENpkRAIFINSlGYVSPCVFTgipa-------------egvkNLYFGNIIekpLPLILKNrnFKEFRKNFLsg--- 309 Thermodesulfovi...
WP_015946609 308 SSCTENvgRAAVITVdGMVAPCVFAalplcggrpr--ddnrtvatgRMLFGSVEdapLAELWRRpaYAAFRAAHArg--- 382 Desulfovibrio v...
WP_012447288 269 RYCNFVesKSLVINYlGKVSPCYPGmhnhtayi-----ygrkkfnyAYHVGDLSqqsIAEIWKQreFIKFRSQVMeg--- 340 Natranaerobius ...
WP_012966572 240 RSCNFVkrRSAVIRWdGEVCPCYRLlhdyaeyi-----fgrkvdvkSFSFGNVRevsLEDIWTSdeYLKFRYVVEns--- 311 Ferroglobus pla...
WP_012309638 237 RWCPFVegDSLAITYeGDVSPCLFAlhdyrawi-----egkevqvrQISFGNLRersLREIWFSedYVIFRSFVKls--- 308 Candidatus Kora...
WP_015740010 248 LYCTENvlRAAFIGVkGTVAPCTFTnlpvttapsf-vrqgktltyePLILGDLNrnsWEEIWSSplYQAFRTSFRkk--- 323 Ammonifex degensii
WP_011697311 273 AVCEENplRNLCISVnGEVTPCVFLcppvpspfkr-iycgtevfvpRVSFGNIFeepFEAIWNDprYVAFRDVFSqreks 351 Syntrophobacter...
YP_002248731 246 EICSENpkRAIFINSlGYVSPCVFTgipa-------------egvkNLYFGNIIekpLPLILKNrnFKEFRKNFLsg--- 309 Thermodesulfovi...
Feature 1                                     #  #
Q51741       303 -------------------------lYPSCTDC 310 Pyrococcus furiosus DSM 3638
ABW02617     286 stsi-------------neysmdlmpPSPCITC 305 Caldivirga maquilingensis IC-167
WP_012546361 310 ------------------------nsSLPCINC 318 Thermodesulfovibrio yellowstonii
WP_015946609 383 ------------------------tpPPPCEGC 391 Desulfovibrio vulgaris
WP_012447288 341 -------------------------rFPSCTDC 348 Natranaerobius thermophilus
WP_012966572 312 -------------------------lYASCTDC 319 Ferroglobus placidus
WP_012309638 309 -------------------------nYPSCHDC 316 Candidatus Korarchaeum cryptofilum
WP_015740010 324 ------------------------dlFPPCREC 332 Ammonifex degensii
WP_011697311 352 fremysalmsdsnrfaalrrhappppPEPCRTC 384 Syntrophobacter fumaroxidans
YP_002248731 310 ------------------------nsSLPCINC 318 Thermodesulfovibrio yellowstonii DSM 11347

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