4K37,4K36


Conserved Protein Domain Family
SPASM_anSME

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cd21120: SPASM_anSME 
Iron-sulfur cluster-binding SPASM domain of anaerobic sulfatase maturating enzyme
Anaerobic sulfatase maturating enzyme (anSME) is a radical S-adenosylmethionine (SAM) enzyme that catalyzes, under anaerobic conditions, the co- or post-translational modification of arylsulfatases to form a catalytically essential formylglycine (FGly) residue to perform their hydrolysis function, removing sulfate groups from a wide array of substrates. Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. Radical SAM (RS) enzymes with a C-terminal SPASM domain contain at least one other iron-sulfur cluster; anSME contains two auxillary 4Fe-4S clusters in its SPASM domain.
Statistics
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PSSM-Id: 410611
Aligned: 35 rows
Threshold Bit Score: 131.246
Created: 22-Jan-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
4Fe-4S cluster4Fe-4S cluster
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1: 4Fe-4S cluster binding site 1 [ion binding site], 4 residue positions
Conserved feature residue pattern:C C C CClick to see conserved feature residue pattern help
Evidence:
  • Structure:4K37: Clostridium perfringens anaerobic sulfatase-maturating enzyme binds auxillary 4Fe-4S cluster nearest the radical SAM site
    View structure with Cn3D
  • Comment:referred to as auxiliary cluster nearest the Radical SAM site or the first auxiliary cluster (AuxI)

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         #     #              #                                                     #   
4K37_A       254 SCGMNGTCtCQFVVESdGSVYPCDFYVldKWRLGNIQdmtMKELFETnKNHEFIKLSfkvhEECKKCKWFRLCKGGCrRC 333 Clostridium per...
Q01S02       268 NCTMHETCdSYVVVEFnGDVYPCDFFVegGWKLGNINedsWGEIARRtRRYSFAAKKtiahPECQVCEFQSICHGGCpKG 347 Candidatus Soli...
WP_015947745 248 SCQWLPHCdSYLVVEHnGDVYPCDFYVydQYRLGNIVredLPAVMHSpLRKKFAAAKaswpGKCRACRYSALCQGDCtRH 327 Desulfatibacill...
CBL00757     254 SCASSGSCgHYLTVEGdGSLYPCDFYVldEWKLGNLShctVEDALDSpTSQTFLAQGrkrpAECAACAYQLLCRGGCkRD 333 Faecalibacteriu...
WP_064591693 258 ACDMVGHCsVNTVIEAdGSVYPCDFYVvdERRLGNINtqsIPEIIFSqKATEFVRESfkidEKCKTCKYLKICRTGCkRH 337 Streptobacillus...
WP_012859859 251 NCAMKGRCeFYYLLEAdGSVYPCDFYVldEWKIGSIAensYSDIKKNsKIKEFEQMShtldKKCTGCEFLYICRGGCrRY 330 Sebaldella term...
EPH59787     216 ACGILGNCqVQYVIEAdGSVYPCDFYVldEFRMGYIQeqtLRELFSQdCSQQFLCQKkdmpSKCTQCPFQKMCRGGCkRM 295 Enterococcus fa...
WP_015751361 251 QCHMHHRCnQYLVVEYnGDVYPCDFFVgpEYCLGNLHdtsFEAMRTSeQYARFAAQKqawpETCAQCAYLELCFGDClKC 330 Desulfohalobium...
WP_054688800 249 TCGMLGECqIQLVIEGdGTVYPCDFYAldQYCLGNITvnnIDEIRQFkSGQIFLKEEkrfsSICKECQFKNICHGQCkRM 328 Coprobacillus c...
WP_008789279 252 TCGFIGQCqPNFIIEAnGSVYPCDFYAldEYQLGNIKeldFKEMSKNkLFSDFSRIEnsipSLCEKCKFSQICHGGCkRM 331 Coprobacillus c...
Feature 1                                      
4K37_A       334 RDSKeds---aleLNYYCqsYKEFFEYAFP 360 Clostridium perfringens ATCC 13124
Q01S02       348 RHGPhgk---fedLDYFCgaYKMYFAKSVG 374 Candidatus Solibacter usitatus Ellin6076
WP_015947745 328 RING---------PSMLCeaWTMLFDHLES 348 Desulfatibacillum aliphaticivorans
CBL00757     334 WDASg--------SNRFCaaYKQFFAYVLP 355 Faecalibacterium prausnitzii SL3/3
WP_064591693 338 KDENn--------VNKFCysYKYFFGRNLD 359 Streptobacillus moniliformis
WP_012859859 331 REQKnsfgksfigLNYFCdsYKEFFSYSLE 360 Sebaldella termitidis
EPH59787     296 KDAMyv------dSEGFCg-YQELLKDFTP 318 Enterococcus faecium 13.SD.W.09
WP_015751361 331 RPTNhr-----gaPSLLCdgWRDFYAATLP 355 Desulfohalobium retbaense
WP_054688800 329 NIVYf--------DDKYCg-YQEFLKDTYQ 349 Coprobacillus cateniformis
WP_008789279 332 RSVYi--------QENYCg-YQDLLEDLLP 352 Coprobacillus cateniformis

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