Conserved Protein Domain Family

cd21109: SPASM 
Iron-sulfur cluster-binding SPASM domain
This iron-sulfur cluster-binding domain is named SPASM after the biochemically characterized members, AlbA, PqqE, anSME, and MftC, which are involved in Subtilosin A, Pyrroloquinoline quinone, Anaerobic Sulfatase, and Mycofactocin maturation, respectively. SPASM occurs as an additional C-terminal domain in many peptide-modifying enzymes of the radical S-adenosylmethionine (SAM) superfamily. Radical SAM enzymes are characterized by a conserved CxxxCxxC motif, which coordinates the conserved iron-sulfur cluster that is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical, which in turn abstracts a hydrogen from the appropriately positioned carbon atom of the substrate. Radical SAM enzymes with a C-terminal SPASM domain contain at least one other iron-sulfur cluster.
PSSM-Id: 410609
Aligned: 124 rows
Threshold Bit Score: 31.2388
Created: 30-Mar-2017
Updated: 25-Oct-2021
Aligned Rows:
putative Fe-S
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1: putative Fe-S cluster binding site [ion binding site], 2 residue positions
Conserved feature residue pattern:C CClick to see conserved feature residue pattern help
  • Comment:partial binding site
  • Structure:4M7T: Bacillus circulans butirosin biosynthetic enzyme BtrN binds [4Fe-4S] cluster
    View structure with Cn3D
  • Structure:4K37: Clostridium perfringens anaerobic sulfatase-maturating enzyme binds [4Fe-4S] cluster
    View structure with Cn3D

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                             #     #
4M7T_A       188 SCMVpsNLAVVTVlGNVLPCFedfnqKMVMGNIGeq-hISDIWHNd----kFTSFRKMLkeghrgksDLC---KNC 255 Bacillus circulans
5V1Q_A       366 GCRAkyTKMEIMSnGDILPCIaflgvNQTKQNAFek-dLLDVWYDdplyggIRSFRTKN--------SKC---LSC 429 Streptococcus suis
Q3A5I2       258 PCEQpfYMMMIHSdGDISMCCvdskkELLLDNITtvdrLATVLKSd----rLKTWRKMHlegdftnlPAC---RHC 326 Pelobacter carbinol...
ABR56005     188 PCLSlyNTLVVSWdGNVTVCCsdvnfNLSIGHIEd--gIINLFNNd----kIKKLRKYHlfgkfdklPLC---KKC 254 Methanococcus aeoli...
NP_711798    253 RCNQpwQRLYIRGnGDVTPCCamfssYLKLGDTTkl-sLVDLWNSk----eAKDLRKIHkegryhenPIC---LKC 320 Leptospira interrog...
P9WJ78       250 MCGAgrVVCLIDPvGDVYACPfaihdHFLAGNVLsdggFQNVWKNss---lFRELREPQs------aGAC---GSC 313 Mycobacterium tuber...
Q8TR79       363 GCPAgrSLMYISAdGDVRACGn---lDHTIGNIRdm-kIMDLWHSd----tMISLRKKPa-------CDCpytDIC 423 Methanosarcina acet...
AAM04721     363 GCPAgrSLMYISAdGDVRACGn---lDHTIGNIRdm-kIMDLWHSd----tMISLRKKPa-------CDCpytDIC 423 Methanosarcina acet...
WP_012548106 237 LCNLg-ESCAIMPnGDVYPCRr---fPIVLGNLTne-sFSDVYERil---kFRAEFTKDnl-----kGKC---HLC 296 Dictyoglomus thermo...
YP_002353596 237 LCNLg-ESCAVMPnGDVYPCRr---fPLVLGNLSqe-sFSKIYPKil---dFRANFTKDkl-----kGKC---FLC 296 Dictyoglomus turgid...

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