3ER8,2GAF,3ER9,3OWG,2GA9,3ERC


Conserved Protein Domain Family
polyA_pol_Pox

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cd20919: polyA_pol_Pox 
Click on image for an interactive view with Cn3D
RNA polyadenylate polymerase catalytic subunit from the Poxviridae family of viruses
Poly(A) polymerases (PAPs) catalyze the attachment of adenylates to the 3' ends of messenger RNA and other RNAs, forming poly(A) tails. PAP acts as a nucleic acid template-independent NMP-transferase, preferentially utilizing a single species of NTP, namely ATP. The polyadenylation state of an mRNA may correlate with the efficiency of its translation. In Vaccinia virus, from the Poxviridae family of viruses, polyadenylation is crucial for virion maturation and is carried out by a heterodimer, formed by the catalytic subunit VP55 and the processivity factor (VP39). In the absence of VP39, oligo(A) tails are added to permissive primers by VP55 in a rapid processive burst, which ceases abruptly after tails have reached 30-35 nucleotides in length. With VP39, tails with lengths in the hundreds of nucleotides are processively synthesized with no abrupt termination of elongation. In contrast to mammalian cells, polyadenylation is not dependent on a multiprotein mRNA 3' end processing complex. VP55 translocates with respect to its single-stranded nucleic acid substrate during poly(A) tail addition. The catalytic subunit of Poxviridae PAPs contains two topologically identical subdomains with a nucleotidyltransferase fold, suggesting that an ancestral duplication was at the origin of these viral PAPs.
Statistics
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PSSM-Id: 410843
Aligned: 65 rows
Threshold Bit Score: 376.915
Created: 16-Oct-2019
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3ERC: Vaccinia virus poly(A) polymerase catalytic subunit binds 3'-deoxy ATP and RNA fragment at the catalytic center; contacts at 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
3ER8_C        19 ETYLGrvp---svNEYHMLKSQARNIQKITVFNKDIFVSLVKKnkkrffsdvnts---aseikdrilsyFSKQTQTYNIG 92  Vaccinia virus WR
3ERC_D        19 ETYLGrvp---svNEYHMLKSQARNIQKITVFNKDIFVSLVKKnkkrffsdvnts---aseikdrilsyFSKQTQTYNIG 92  Vaccinia virus WR
AVD69190       8 NRYLGkta---dqPIYYALFHKVGKIKQILNFDLNVFLKLLLKnrdrflrenkqp---taeikrrlthyFTKQHRVRKVG 81  Saltwater croco...
YP_008003861   2 DLYFKkkiskelaSSLSKHENKIKIINAFKAKELINNLCRMRDifiensfvlckd---ediakrveelfSRHLKPDLKLG 78  Adoxophyes honm...
NP_048214      2 NTYINtre---fdNELIKYKQQIDTIVKFDKDEFIKKLIIIKDiyitdfinisk----nndikrrvytyFNKQNTFDKIG 74  Melanoplus sang...
YP_008004468   2 NIYLKnisn-efnIQLLKYKNQINDILSFDINNFTKNILIMRDifindknifgessnddnikrrineffDKQNTPNLKLG 80  Choristoneura r...
YP_009001619   2 NIYIRnkpl-dikNALVDYNDKINDIVKFDKNKFIKQLISIRDiyvndfidtskn---ddisrrvneyfDKQNVQNLKLG 77  Anomala cuprea ...
YP_008003581   2 DKYLFnasn-dtiNQLNKFKNQINDILSFNVDNFTKNLFLMRDifvrdfetsatn---dnikrrvyeffNKQNNPNLKTG 77  Mythimna separa...
NP_064820      2 NIYLKnasn-dtiSHLSKFTNQINDIISFDINNFTKNVLIMRNninnirtnfenvsddnsikrriteffDKQNTPNLKLG 80  Amsacta moorei ...
YP_784244      8 NRYLGkta---dqPIYYALFHKVGKIKQILNFDLNVFLKLLLKnrdrflrenkqp---taeikrrlthyFTKQHRVRKVG 81  Nile crocodilep...
Feature 1                                                                                        
3ER8_C        93 KLFTIIELQSVLVTTYTDILGVLTIKAPnvisskisYNVTSMEELARDMLNSMNVAVIDKAKvmgr-------------- 158 Vaccinia virus WR
3ERC_D        93 KLFTIIELQSVLVTTYTDILGVLTIKAPnvisskisYNVTSMEELARDMLNSMNVAVIDKAKvmgr-------------- 158 Vaccinia virus WR
AVD69190      82 KILSIVEFQHVIVTTFTRVLGVLTIDNRrvskmyssTAILDYAAHEDYVDAMLRSYRVTDGSgppkg------------- 148 Saltwater croco...
YP_008003861  79 TIIAIINFQCLTATYVNKTLEEIIVYKNntteinivNFSTVTSWINNNDNVILDKLLKQYVYkqklknksneni--kkyd 156 Adoxophyes honm...
NP_048214     75 SIIAVLKFQHLIVTYVNRLLEDIIDHNNgsisvelmNFTSITSKTVKKSDESIAPILKVTSNylcrsrlinknq-eipkn 153 Melanoplus sang...
YP_008004468  81 SIISIIKFQHLTVTYVNKIIQEILTYKFntreinivNFSSVTSWITNYENPILDEIMKQYVYkqklknvtvnnnvknfin 160 Choristoneura r...
YP_009001619  78 SIKSIIKFQHIIVTYVSKLLDNIIIYNNsskmvdmiNFLSLTSKIVPKDNDKLNNILKEYIYnsklkniskqik-knent 156 Anomala cuprea ...
YP_008003581  78 NIISIIKFQHLTVTYVNKLMQEILTYKIntkeinliNFSSVTSWITNQDNPLLNDILKQYVYnqklknitpksk-kkvhn 156 Mythimna separa...
NP_064820     81 SIISIIKFQHLTVTYVNKIIKEIVTYKCntreinivNFSSVTSQISNYDNPILNEILKQYVYkqklknvtvnndkkkiid 160 Amsacta moorei ...
YP_784244     82 KILSIVEFQHVIVTTFTRVLGVLTIDNRrvskmyssTAILDYAAHEDYVEAMLRSYRVTDGAgppkg------------- 148 Nile crocodilep...
Feature 1                                        ##           ### #                              
3ER8_C       159 ----hnvSSLVKNVNKLMEEYLRRHNksCICYGSYSLYLINPNirYGDIDILQTNSRTFLIDLAFLIKFITGNNIILSKI 234 Vaccinia virus WR
3ERC_D       159 ----hnvSSLVKNVNKLMEEYLRRHNksCICYGSYSLYLINPNirYGDIDILQTNSRTFLIDLAFLIKFITGNNIILSKI 234 Vaccinia virus WR
AVD69190     149 ---rnkvSDLVGYVIAMLKEYLKRHNksVFCHGSYSLHLLNPAieYGDIDMLQTNSRTFLINLAFLIYCNTGRVTTMMKI 225 Saltwater croco...
YP_008003861 157 vfdeeivEKLKKLIEKILKILLVIKNndCVCYGSFTCYNINRKikYNDIDLYSTDAYRILSFFMIYIYLSTGYDTCLFSI 236 Adoxophyes honm...
NP_048214    154 knvsddvINICKLLENILKTILVIKNheCLVYGSFTCFNIDSNikYNDIDLYSINAYKLMIFFMSIINFLIGYDVYIFSI 233 Melanoplus sang...
YP_008004468 161 kedeesaEKIKKILEEILKVLLIIKNndCVAYGSFTCYNINRKikYNDIDLYSTDSYRILIFFMIYTYIIIGYETCLFSI 240 Choristoneura r...
YP_009001619 157 ehdisiaTKIGNILENILKTILIIKNndCLCYGSFTCYNINCNikYNDIDLYSTDAFRIVMFFMIIIHFIIGYDTYMFSI 236 Anomala cuprea ...
YP_008003581 157 kedldtsEKIKKILEELLKIILIIKNndCVSYGSFTSYNINRKikYNDIDLYSTDSYRLLIFFMIYIFLSIGYDTCLFSI 236 Mythimna separa...
NP_064820    161 pddeklaESIKKILEEILKILLIIKNndCVAYGSFTCYNINRSikYNDIDLYSTDAYRILIFFMIYIHLTIGHDTCLFSI 240 Amsacta moorei ...
YP_784244    149 ---rnkvSDLVGYVISMLKEYLKRHNksAFCHGSYSLHLLNPAieYGDIDMLQTNSRTFLINLAFLIYCNTGRVTTMMKI 225 Nile crocodilep...
Feature 1         #                #                              #  ##     #         #   #      
3ER8_C       235 PYLRNYMVIKDEndnHIIDSFNIRQDtmnVVPKIFIdNIYIVDPTFQLLNMIKMFSQIDRLEDLskdpEKFNARMATMLE 314 Vaccinia virus WR
3ERC_D       235 PYLRNYMVIKDEndnHIIDSFNIRQDtmnVVPKIFIdNIYIVDPTFQLLNMIKMFSQIDRLEDLskdpEKFNARMATMLE 314 Vaccinia virus WR
AVD69190     226 PYLLNYIVMFDEeqaHIVDSFQVSQEifdRIPKILInDIYIVDPCVQLLNGIKMFSQVDRLDDLhtkfEKLRSRFCTLLE 305 Saltwater croco...
YP_008003861 237 PYIIGHISLKYKn-iFIIDCIFLDKStidSINKVLInNIYFIDPGLQMLNNFRMLSENFRSYKIyeklEESLNKYITLLN 315 Adoxophyes honm...
NP_048214    234 PFIEGHISLKYKd-vVLIDCIFLPKKiidNIPKVMInNIYFIDPGMQTLNNFRMLSENFRSFKLtkdfENSYKKYIILIE 312 Melanoplus sang...
YP_008004468 241 PFIVGHISLKYKn-vVIIDCIFLDNStinTINKVLInNIYFIDPGLQMLNNFRMLSENFRSYKIyekmEESLYKYKTLLN 319 Choristoneura r...
YP_009001619 237 PFILGHISLKYKn-iFLIDCIFMDKPtlnCIPKVLInNIYFIDPGYQMLNNFRMLSENFRSYKInediESAYIKYKTLLN 315 Anomala cuprea ...
YP_008003581 237 PYIVGHISLKYKd-iFIIDCIFLDNYtinVINKVLInNIYFIDPGLQMLNNFRMLSENFRSYKIyqnmEESLYKYKTLLN 315 Mythimna separa...
NP_064820    241 PFITGHISLKYKn-iFIIDCIFLDNSiinVINKSLInNIYFIDPGLQMLNNFRMLSENFRSYKIyekmEESLNKYKTLLN 319 Amsacta moorei ...
YP_784244    226 PYLLNYIVMFDEeqaHIVDSFQVSQEifdRIPKILInDIYIIDPCVQLLNGIKMFSQVDRLDDLhtkfEKLRARFCTLLE 305 Nile crocodilep...
Feature 1                                                                                        
3ER8_C       315 YVRythgivfdgkrn------nmpmkciidenNRIVTVTTKDYf---sFKKCLVYLDENVLSSDILDl----------NA 375 Vaccinia virus WR
3ERC_D       315 YVRythgivfdgkrn------nmpmkciidenNRIVTVTTKDYf---sFKKCLVYLDENVLSSDILDl----------NA 375 Vaccinia virus WR
AVD69190     306 YVLydydmrigegsg-----agelrrsrfayaERVATVEAGALgedlsPARWVAFMDNAALDARIAA-----------ST 369 Saltwater croco...
YP_008003861 316 YFVnnnnkfnknrlncwlksditkanfpyeivDNAVVISIKKLidtspFDYIMIVMDSPSVIMEKLSklnglfskkygAF 395 Adoxophyes honm...
NP_048214    313 YFIqnsmfnikrlkeif-sntytkksipytinNTSLTIDIKKFipsslYDYVIVIWNSPDHIMKFLSkikgrfsmkygSF 391 Melanoplus sang...
YP_008004468 320 YFVnnnnkfnkqrlnhwmtvdinkenfpysivDNTILISIKELidiseFDYIMIVLDSPSVVMEKLSkinglfsrkygAF 399 Choristoneura r...
YP_009001619 316 YFTlnnsfnnnrlnnwl-skkltmkdipyyieNYNIIIDIKKIietspYDQIIIILGEPYRIMNELKklngnfsrkygSF 394 Anomala cuprea ...
YP_008003581 316 YFVnnnnkfnkdrlniwlnsgitretfpykivDNTILISIKELidispFDYIMIVLDSPNKIMDKLSkinglfsrkygAF 395 Mythimna separa...
NP_064820    320 YFVnnnnkfnkqrlnywlksdvcrnnfpytivDNTILISIKELidispYDYIMIVLDSPSDIMEKLSnisglfsrkygAF 399 Amsacta moorei ...
YP_784244    306 YVLydydmrigegsg-----agalrrsrfaysERVATVEAGALgedlsPARWVAFMDNAALDARIGA-----------ST 369 Nile crocodilep...
Feature 1                                                   # #                # #               
3ER8_C       376 DTSCDFE-----------------------------------SVTNSVYLIHd--nIMYTYFS-NTILLSdkgk------ 411 Vaccinia virus WR
3ERC_D       376 DTSCDFE-----------------------------------SVTNSVYLIHd--nIMYTYFS-NTILLSdkgk------ 411 Vaccinia virus WR
AVD69190     370 RQAADFG-----------------------------------PVTNSRFLEEd--gCLYGYFS-NTLLLTpdga------ 405 Saltwater croco...
YP_008003861 396 INEIFFEtkknrniktnscagns----itqiidenkliklerKDINMPYNINp--nKKYLIFSnLTTSTYvyaennkiid 469 Adoxophyes honm...
NP_048214    392 LNEIFFEtsissdkiskmdiiagnvvcekspyvidentmkkiNVTNDEIDIYidttKKYLIFTnLTTSTYlyvendkvvd 471 Melanoplus sang...
YP_008004468 400 LNEIFFEtkksknniiaragnvn---nitqlidenklikldrNDITMPYNINp--nKKYLIFSnLTTSTYvysdndnkii 474 Choristoneura r...
YP_009001619 395 LNEIFFEtsltdknkiiisggqh---kytqivdenklsrikiTSIDNKLNIDs--kKKYLIFSnLTTSTYlyinndvkel 469 Anomala cuprea ...
YP_008003581 396 INEIFFEtkkiksnksnhnyagnv-nivtqlidenkliqldrNNIDLPYNINs--nKKYLIFSnLTTTTYvkyednkivd 472 Mythimna separa...
NP_064820    400 LNEIFFEtkkiknkintyagntn---nitqlidenkliklnrSDINMPYNINp--nKKYLIFSnLTTSTYvyfendkmtd 474 Amsacta moorei ...
YP_784244    370 RQAADFG-----------------------------------PVTNSRFLEEd--gCLYGYFS-NTLLLTpdga------ 405 Nile crocodilep...
Feature 1                                                                        #          
3ER8_C       412 ---------vheiSARGLCAHILLYQMltsgeYKQCLSDLLNSmmnrdkipiySHTERDKKPGRHGFINIeKDII 477 Vaccinia virus WR
3ERC_D       412 ---------vheiSARGLCAHILLYQMltsgeYKQCLSDLLNSmmnrdkipiySHTERDKKPGRHGFINIeKDII 477 Vaccinia virus WR
AVD69190     406 ---------phpvTCNALAAHFLMYFVmtgvpCKPQLACLLNSlvvpe-arefTLVPRDKKLGDHVILSIdHDVF 470 Saltwater crocodilep...
YP_008003861 470 i-svknlisfiatACLYNLLHKRDNFGme--lYYLILHCLTFDetrr--lneyKVISRYKIQGKHIEISLcKNLF 539 Adoxophyes honmai en...
NP_048214    472 i-smknlvsfiatISLYNFLHKNNEFAmd--lYYYLFALLRIEddrf--ieeyTIFPRYKRKGEHIEISMyKNIF 541 Melanoplus sanguinip...
YP_008004468 475 disvknlisfiatTCLYSLLHKKDDFGme--lYYLTLQCLTFEetrk--lneyKVISRYKIKGEHIEISLcKNLF 545 Choristoneura rosace...
YP_009001619 470 s--lknlisflstTSLYCLLHKKCDFGme--lYYCIISLLTLHdtrk--iteyKVLDRYKLEGKHIEISIsKNLF 538 Anomala cuprea entom...
YP_008003581 473 i-svknliafiatTCLYNMLHKRDEFAme--lYYLTLHSLTFDepmk--lldyIKFDRYKMQGDHKEISLcKNLF 542 Mythimna separata en...
NP_064820    475 i-svknlisfistACLYNLLHKKDDFGme--lYYLTLHCLTFTetrk--lneyKVIDRYKIKGEHKEISLcKNLF 544 Amsacta moorei entom...
YP_784244    406 ---------phpvSCNALAAHFLMYFVmtgapCKPQLACLLNSlvvpe-arefTLVPRDKKLGDHVILSIdHDVF 470 Nile crocodilepox virus

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