4P37,4WSE,3ER8,3ER9


Conserved Protein Domain Family
polyA_pol_NCLDV

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cd20918: polyA_pol_NCLDV 
Click on image for an interactive view with Cn3D
RNA polyadenylate polymerase of nucleocytoplasmic large DNA viruses
This model represents the poly(A) polymerases (PAPs) from nucleocytoplasmic large DNA viruses (NCLDV), a group of giant eukaryotic double-stranded DNA viruses that make up the phylum Nucleocytoviricota. They are referred to as nucleocytoplasmic because they are often able to replicate in both the host's cell nucleus and cytoplasm. PAPs catalyze the attachment of adenylates to the 3' ends of messenger RNA and other RNAs, forming poly(A) tails. PAP acts as a nucleic acid template-independent NMP-transferase, preferentially utilizing a single species of NTP, namely ATP. The polyadenylation state of an mRNA may correlate with the efficiency of its translation. This group includes PAPs from the Poxviridae and Mimiviridae family of viruses. In Vaccinia virus, from the Poxviridae family, polyadenylation is crucial for virion maturation and is carried out by a heterodimer, formed by the catalytic subunit VP55 and the processivity factor (VP39), which is required for the formation of long poly(A) tails. PAPs from Acanthamoeba polyphaga mimivirus and Megavirus chiliensis, which belong to the Mimiviridae family, are homodimeric and intrinsically self-processive, generating >700 nucleotides long poly(A) tails. Homodimerization is required for PAP activity; monomers are able to bind RNA but are enzymatically inactive. Thus, while other PAPs form heterodimers with processivity factors, the Mimiviridae PAPs become processive upon homodimerization. The catalytic subunit of NCLDV PAPs contains two topologically identical subdomains with a nucleotidyltransferase fold, suggesting that an ancestral duplication was at the origin of these viral PAPs.
Links
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Statistics
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PSSM-Id: 410842
Aligned: 10 rows
Threshold Bit Score: 252.362
Created: 17-Aug-2020
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP binding
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:3ER9; Vaccinia virus Poly(A) polymerase catalytic subunit with bound 3'-deoxy ATP, contacts 4A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                             # #                      ## #                              
4P37_A        53 RVVXEIIKDFIRDKG---RKVYGGTALNETIKKknpedaiydsylFSDIEFYSPTpv---pDLKELCDILYHKGydPVQG 126 Megavirus chili...
4WSE_A        50 RAIMEVIKDFIRDHQ---RKVYGGTALNEALKQvnpkdaiydnysFSDIEFYSPTpv---qDLVDLCNILYRKGykFVQG 123 Acanthamoeba po...
QBK87307      56 KEAYKIVSNFVKSKN---LKLYGGTALNIYLPQkhkiy---kkstLPDYDFFSPDpw---nDAVELADLLYKAGfkYTET 126 Marseillevirus ...
YP_008052392  36 TPIINILEKFLRSNA---SLCYGGTAINNILPEkdrfy--nkdieIPDYDFFSPNal---tYAKKLADLYYAAGytEVEA 107 Phaeocystis glo...
AMN83802      26 LKAIDIIKKFIVNRG---LIVYGGTAIDYALRAhgdkiypddlllVPDLDFYSPDnv---nDAYDLATQLYAAGytEARV 99  Faustovirus
P0C9D2        29 EAALEVVREFIIKKK---LILYGGIAIDYALHLkgssi--ypegeRPDFDMFSPNhv---eDAYELADILYEKGfkQVGT 100 African swine f...
3ER8_C       166 KNVNKLMEEYLRRHNk-sCICYGSYSLYLINPNi----------rYGDIDILQTNsr---tFLIDLAFLIKFITgnNIIL 231 Vaccinia virus WR
3ER9_B       166 KNVNKLMEEYLRRHNk-sCICYGSYSLYLINPNi----------rYGDIDILQTNsr---tFLIDLAFLIKFITgnNIIL 231 Vaccinia virus WR
AUF82742      21 TTVNNARWQCVDVAKklgLTFHGGFAINNCLSSknklf---ddtdIPDIDTMSSKnnnskkTAFVVADELRKRGikQVRV 97  Tetraselmis vir...
GAX85147      29 EDRVFAKKIALETAQkngWLVYGGSAIDDMLPAdekly---nypfVGDVDIMCPDpe---pASKELAWNLHNSGiaFVES 102 Chlamydomonas e...
Feature 1                             #                                   #  #   #               
4P37_A       127 KEAqh-eETYSIFVNl-qLYCDITYVPTkvyhGIKTIEId--GINYTH------PHFXLIDYLRXINQpLTAAEQr---- 192 Megavirus chili...
4WSE_A       124 KDAqh-eETYSIFVNf-qLYCDITYSPTrvfyGIKTIEId--GINYTD------PHFMLIDYLRMVNQpLTAAGQr---- 189 Acanthamoeba po...
QBK87307     127 KAGih-kGTFKVFANf-wPIADITYLPQdlydQITTKKKn--GYTIVS------PAYLQMTMYNMISKpIESPVRw---- 192 Marseillevirus ...
YP_008052392 108 KAGih-sGTYKVYVNf-iPIADITYIDKaifsNLLKNSIkvnGISYCP------PDFLRMSMYLELSRpMGDVSRw---- 175 Phaeocystis glo...
AMN83802     100 INAkh-tGTMKVDIGdnhFLADISYLPKvlydVIPTLEYe--HMRIVH------PNFQALDQHHDLSSpFSSAPTeevfn 170 Faustovirus
P0C9D2       101 VRAih-vQTMRVRTDf-vWVADLSYMPPnifdTIPTLTYk--NLKIIH------PDYQRAGLHLAFCFpFDNPPRedvfs 170 African swine f...
3ER8_C       232 SKIpylrNYMVIKDEndnHIIDSFNIRQdtmnVVPKIFId--NIYIVD------PTFQLLNMIKMFSQiDRLEDLsk--- 300 Vaccinia virus WR
3ER9_B       232 SKIpylrNYMVIKDEndnHIIDSFNIRQdtmnVVPKIFId--NIYIVD------PTFQLLNMIKMFSQiDRLEDLsk--- 300 Vaccinia virus WR
AUF82742      98 LQAih-qHTYSVRMDr-tVLIDISHIKQs--eMNSLNSIa--KIEGVYekrsvpPSYLKMSMHLELCRpKMFVERw---- 167 Tetraselmis vir...
GAX85147     103 RPSrandGTYNIKGMhigSIADVSFIDInafnALKRKST---KEPFVP------YEYTLCAFHCEFSQpHRSPERh---- 169 Chlamydomonas e...
Feature 1           #   #                                                                        
4P37_A       193 -wEKAFDRXYVLLKNYPxekydnsxritsprddiq---xyigkvksefxkipeiqESCLISGFDAYnffirhaxgdrkve 268 Megavirus chili...
4WSE_A       190 -wEKAFERMYRLLKDYPiedfdkrldipeppeeiq---syisrikteflsdnklnESFLISGIEAYnfyirhaasskdee 265 Acanthamoeba po...
QBK87307     193 --PNVSFRQKLLEQWAApkyrkkqcaddfigekerpelepelekalqvvhrevrkAKLIHYGALAFnkyialgg------ 264 Marseillevirus ...
YP_008052392 176 --EKVLKRLVLLNKHHPlsgdncskekfarkydgs---kaaasdiynivrssvisQGLVFFGGYASglygkymsa----- 245 Phaeocystis glo...
AMN83802     171 rwSKDLKRFNILSKYFPlltgkipnelavktkd------yhlnqinipldnisgaIGGIPALKLAYiqlneylag----- 239 Faustovirus
P0C9D2       171 rfKKDLQRYNLIEKYYPipvvpvkttyes---------------ktfsvpfkqvaIHGFAAYALLYqtlnelr------- 228 African swine f...
3ER8_C       301 dpEKFNARMATMLEYVRythgivfdgkrn---------------nmpmkciidenNRIVTVTTKDYf------------- 352 Vaccinia virus WR
3ER9_B       301 dpEKFNARMATMLEYVRythgivfdgkrn---------------nmpmkciidenNRIVTVTTKDYf------------- 352 Vaccinia virus WR
AUF82742     168 --KKIYPRLTAIYQDYPslikdytissdlilte-------pepnpireafhtldfNKHFCLVGSDSvhaltgkwy----- 233 Tetraselmis vir...
GAX85147     170 --SKLLYRFGKLFAAAPfifdpltlqkdvtnsg-------vkskkkvlsamkkagLKKPFVGVSASleslg--------- 231 Chlamydomonas e...
Feature 1                                                                                        
4P37_A       269 qxarikndynslknfitvlPFXELISVkykdtveklynflrekvvnpdlitiDEYFPL-----------FQFTGYSVSIn 337 Megavirus chili...
4WSE_A       266 qmartnrnvvnlnnfianvPFSELISVnyredvkntynflrmivedkekisvDEYFPL-----------FQFTGYSTVIk 334 Acanthamoeba po...
QBK87307     265 ------------gelripvYFYELLTEdagkyakefekq-ikkitmnelindVVYLPYkd-------mnKLSYILYMVId 324 Marseillevirus ...
YP_008052392 246 ----------kerkqivgiPDFDILSTdaessavi----------ikeqlihAGYDTVtinkk--pgvgELITAHYEIMi 303 Phaeocystis glo...
AMN83802     240 ----------ksvervsikELDDLIFDyknq------------------titASCVVYfd--------iHSMNSLSMVDk 283 Faustovirus
P0C9D2       229 ------------itckapeWKTEFPQPs------------------------YSYHKNdknitltvdmpRAYPSLVLATy 272 African swine f...
3ER8_C       353 ------------------sFKKCLVYLdenvlssd----------ildlnadTSCDFE-----------SVTNSVYLIHd 393 Vaccinia virus WR
3ER9_B       353 ------------------sFKKCLVYLdenvlssd----------ildlnadTSCDFE-----------SVTNSVYLIHd 393 Vaccinia virus WR
AUF82742     234 ----------lrdwpvdmmFHQDDIRDadsfvng------------lihtldLDYPAHktk------etLFLPEYYLLSk 285 Tetraselmis vir...
GAX85147     232 ---------------lepiAEFEVLSDtpl--------------------edAQMLSLepari--tgtsSGLQGLPEEHh 274 Chlamydomonas e...
Feature 1                                                                                        
4P37_A       338 ydg-------ipiVKVYEADg-yCVPDIkttsg------------yryvSYQYILXIXYISKFkahldkn-----kexyf 392 Megavirus chili...
4WSE_A       335 ydd-------hpiIRIYEGDg-yCIPNVktvktvendngtktkyeykyvSFQYVLMILYINKFrahldkn-----kpmyf 401 Acanthamoeba po...
QBK87307     325 enm-------ypvFSITELTr--CIPYKyfgg-------------ryycSIDYLFYELYNQMFnedtdlhsfdvscliry 382 Marseillevirus ...
YP_008052392 304 thn------nltdVICYVYNtssCHSYNnitidg---------etlkiaSIDTMLSFYLVFIYidrpyy-------dvnr 361 Phaeocystis glo...
AMN83802     284 h-----------lINDVEYRr--NLAGAim------------------pHYIGVIANTKPPIRni--------------r 318 Faustovirus
P0C9D2       273 npeevikemglhlTEICEPYm--DYSPPi--------------------FKTKDIHFFSTMFKelaismi-----qdnii 325 African swine f...
3ER8_C       394 ------------nIMYTYFSn--TILLSdkgk-------------vheiSARGLCAHILLYQMlts-------------g 433 Vaccinia virus WR
3ER9_B       394 ------------nIMYTYFSn--TILLSdkgk-------------vheiSARGLCAHILLYQMlts-------------g 433 Vaccinia virus WR
AUF82742     286 ngay----vgrvfVTNTEVSt--AKKEDegp---------------vfgSTDLVLQLLYGQYLknytad-------kdtk 337 Tetraselmis vir...
GAX85147     275 itleg---rslrvQMSFLKNr-yCHHYVeigkn------------elrgGAHTLLYFLFMRLLfddevq------vplvr 332 Chlamydomonas e...
Feature 1                                                
4P37_A       393 nYGIAISNLVQArnsylnqknigv-indtvfsefriGCIG 431 Megavirus chiliensis
4WSE_A       402 nYGIAISNLVKArniyldqtgksv-ldntvfkefrtNCTG 440 Acanthamoeba polyphaga mimivirus
QBK87307     383 lYYIQQRYYSTKniseldks---------pfqrfviKCRG 413 Marseillevirus LCMAC201
YP_008052392 362 lLCMSEYLFRVQlknrleqk--------gllkrfsiDCYG 393 Phaeocystis globosa virus
AMN83802     319 vYDAKGTNVSINtyeikdtglrlrfsniqltlrtllGNYY 358 Faustovirus
P0C9D2       326 vVSPQYLLLYFLygafat------------padkslFLFY 353 African swine fever virus Malawi LIL 20/1
3ER8_C       434 eYKQCLSDLLNSmmnrd--------------kipiySHTE 459 Vaccinia virus WR
3ER9_B       434 eYKQCLSDLLNSmmnrd--------------kipiySHTE 459 Vaccinia virus WR
AUF82742     338 dRIISSINDLVCiqy-------------------taSCDA 358 Tetraselmis virus 1
GAX85147     333 sVIGALYQELFTtnvppka-----------lrtfeeDARC 361 Chlamydomonas eustigma

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