6Q0R,6Q0V,6Q0W,6PAI


Conserved Protein Domain Family
DCAF15-CTD

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cd20913: DCAF15-CTD 
C-terminal domain of DDB1- and CUL4-associated factor 15
This model represents the C-terminal domain of DCAF15 (DDB1- and CUL4-associated factor 15), the cullin RING ligase substrate receptor/adaptor that forms a complex with CUL4A or CUL4B, as part of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ubiquitin ligase that is responsible for the proteasome degradation of certain proteins. Aryl sulfonamide anticancer agents such as indisulam, tasisulam, E7820, and chloroquinoxaline have been shown to recruit the essential mRNA-splicing factor RBM39 to DCAF15. These agents appear to promote binding of DCAF15 to the RNA-recognition motif (RRM) of RBM39, which suggests that derivatives of the aryl-sulfonamides may be used to target other RRM-containing proteins. Cell proliferation is inhibited by these aryl sulfonamides by causing degradation of RBM39, which leads to aberrant processing of pre-mRNA in hundreds of genes, primarily reflected by intron retention and exon skipping, thus collectively referred to as splicing inhibitor sulfonamides, or SPLAMs.
Links
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Statistics
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PSSM-Id: 411023
Aligned: 69 rows
Threshold Bit Score: 122.779
Created: 17-Aug-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:oligomer interface [polypeptide binding site]
Evidence:
  • Comment:DCAF15 is a component of the Rbx-Cul4-DDA1-DDB1-DCAF15 E3 ligase complex
  • Structure:6PAI: Human DCAF15 interacts with DDB1 and DDA1 within the DDB1-DDA1-DCAF15 core E3 ubiquitin ligase complex; contacts at 4.0A
    View structure with Cn3D
  • Structure:31686031
    View structure with Cn3D
  • Structure:6Q0R: Human DCAF15 NTD/CTD interacts with DDA1 and DDB within the core E3 ubiquitin ligase complex; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
6Q0R_C        50 VNYTKLYYVLesge----gtepEDELEDDk--------iSLPFVVTDlRGRNLRPmrert-------avqgqYLTVEQLT 110 human
6PAI_C       285 VNYTKLYYVLesge----gtepEDELEDDk--------iSLPFVVTDlRGRNLRPmrert-------avqgqYLTVEQLT 345 human
XP_028513315 334 TNYSYKVYYKtds-------edVFDDCDDii------nhEIPVNLLS-NNQPLSScdtiy-------gvtepSVLVCQAF 392 Exaiptasia pallida
XP_013782527 662 VKETRQIFSQrrvnr---yrneEDSSEEEaqns--eyhsSLPLEVHGtTYQPLESgfkgnl-----maqdefIVAVRQVA 731 Atlantic horses...
XP_031566653 365 TTYSYRKFTTpcd-------vqDNSFDTSde------tvEVPALVMH-GTQELESviysehkg--pnsssgpSILVCQAL 428 Australian red ...
XP_028403865 376 SSFSFKTFSNrtdp-----kilENISTSTvid----gtsYLDIQVNTaSGTPYKLleniqdnk-evkdhtspCIHTRVIS 445 Dendronephthya ...
XP_004212208 202 VSYVIQGFSLq----------nEEELEESpesffdctsiVLPVQVFF-NDKRLNVscehrcfvdqsssiidaDFVVGQAQ 270 Hydra vulgaris
RDD47787     361 VRRIRQVYYAmp---------dESNLEISasssf-dvenYFDLNIYNtKRQRLYLqpqdse------llekdALITHQLA 424 Trichoplax sp. H2
XP_021964604 429 TARVTRSYVLlneesvsttteiEDNEYNFnef-----pvALPTNVYT-DQTQLSMipnyk--------avdkKVLITQTS 494
XP_019857646 230 CTIVHQSFLLprgdn--asatnVYSSLTSls------qvVLPVEVYY-KERALVEvslpsss----stdpemRVNVSQYC 296
Feature 1                                          # #      #                                  # 
6Q0R_C       111 lDFEYVINEVIRHd-aTWGHq-FCSFsDYDIVILEVCPetNQVLINIGLLLLAFPsptee------gqlrPKTYHTSLKV 182 human
6PAI_C       346 lDFEYVINEVIRHd-aTWGHq-FCSFsDYDIVILEVCPetNQVLINIGLLLLAFPsptee------gqlrPKTYHTSLKV 417 human
XP_028513315 393 lDMEECINDLIQSh-eELRDr-YKSLrDYDCQLLSVCPe-GEVIVMVNVLVNAKRklschsy-glqvspsPGLHQTGFVF 468 Exaiptasia pallida
XP_013782527 732 fDIEQFAHEMAQRlcaEAGRk-YLAFnDYDVQVIDLCPesQTAVVLVLMLIQATVgnkgvak--glrnaeRHVFQTGFKF 808 Atlantic horses...
XP_031566653 429 lDMEQCISELIASn-eDLYHr-YKSLrDYDCQFLGVCEdtGDVIVMVKILMFAKPtrsslsd-glqvspsPLLQQTGFVF 505 Australian red ...
XP_028403865 446 lDIERYINEALNTs-dELKGk-YWALrNYSTLLVDVCEgsQCVIVNIAIILSVQEdtek--------atrREISSFSLLL 515 Dendronephthya ...
XP_004212208 271 fNAETFLNEKIQEi-eEVKEr-YVSLrDYDMQFAEVCEttNEVVILLNALVILREnvnacgdvlnqrsnkLLLYRFGYIV 348 Hydra vulgaris
RDD47787     425 lDIECCIGAIAELl-pYLQEk-ELSLaDYDLQIIDVCPftMKLTILIVALFKGTQppkrh-----hrknwQSCCRLDCVL 497 Trichoplax sp. H2
XP_021964604 495 lDFEILSNEIANIicnKEGAq-FAFLiDFDAIIVQTHPidCTITALVILLIQATTakden------iennRLSYETNYII 567
XP_019857646 297 fDIEPWLVSTLEDillKTVHvnLSSLnDYEMIICRVCP--EDKIVCLLVKVNAASfseg--------tplYQGYSIKLMC 366
Feature 1                 ##### #                               ##       ##                      
6Q0R_C       183 AWDLntgIFETVSVGDLTEVKg------qTSGSVWSSYRKSCVDMVMKWLvpessgRYVNRMTNE-ALHKGCSLKVLADs 255 human
6PAI_C       418 AWDLntgIFETVSVGDLTEVKg------qTSGSVWSSYRKSCVDMVMKWLvpessgRYVNRMTNE-ALHKGCSLKVLADs 490 human
XP_028513315 469 SWNIwngQYRTLHYMDVEDHPsh-----gFTKSESSMEKARCLRKDTAIPvg--frSQVRVFSNH-TVFSDKSLKYLLHp 540 Exaiptasia pallida
XP_013782527 809 QWKIdsgTYSIIDTEELRQIDpydvgkyeSLSHVWNPAQQAVKHLQQQWYiphahaRSVNVLTNE-AVFQGTSKKVLWAp 887 Atlantic horses...
XP_031566653 506 SWNVwngTLQTLYCLETQDHPq------gKSLSRSESTVEYARSLRQGTTvpvgfsSQVRVFSNY-TVFTEKSLKYLLHp 578 Australian red ...
XP_028403865 516 SWSVstgEVNTLDISPIAKTSs------kSEKQIFKEGCKIVADLHRRYSvpfpglFAVQALSNH-NVFTGKTLEVIKHp 588 Dendronephthya ...
XP_004212208 349 TWNVy-tNRITVLHAMKPILDgvg---seNKSQNFSPSLSAMIQLRKKWFipvgkhASVQSLSNH-TVFTGSSLPRIHHp 423 Hydra vulgaris
RDD47787     498 SWSLk--TNKIWLTGDPKVSPief--palHQSQKWDPASTLLKTLLKRCHsplnpfSSVRILTNS-TVFTGVPLSVLLSp 572 Trichoplax sp. H2
XP_021964604 568 QWNPsaqSAKVLFKGKLQVASn-------KFDQVWNPTQALALSLQSMRLyk---qDNPHLNNNQ-MVYDWISADELSDs 636
XP_019857646 367 EWNLl--KNEARLITHSHPLPi-------SLTQLNKPLDGRSFEIDKSDLlf---ySPVHGITNSsVLSTGESLLRLDHd 434
Feature 1                            ##   
6Q0R_C       256 e------------------rYTWIV 262 human
6PAI_C       491 e------------------rYTWIV 497 human
XP_028513315 541 f------------------lPIVLV 547 Exaiptasia pallida
XP_013782527 888 a------------------hHLAIV 894 Atlantic horseshoe crab
XP_031566653 579 f------------------lPIIII 585 Australian red waratah sea anemone
XP_028403865 589 f------------------qPIAIV 595 Dendronephthya gigantea
XP_004212208 424 f------------------fPITII 430 Hydra vulgaris
RDD47787     573 t------------------eAVAIT 579 Trichoplax sp. H2
XP_021964604 637 kgvvtlyreviqtsaeeyfdDDDII 661
XP_019857646 435 flp-------------lclvYSEPD 446

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