Conserved Protein Domain Family

cd20851: C1_DGK_typeI_like_rpt2 
second protein kinase C conserved region 1 (C1 domain) found in type I diacylglycerol kinases
Diacylglycerol (DAG) kinase (EC is a lipid kinase that phosphorylates diacylglycerol to form phosphatidic acid. Type I DAG kinases (DGKs) contain EF-hand structures that bind Ca(2+) and recoverin homology domains, in addition to C1 and catalytic domains that are present in all DGKs. Type I DGKs, regulated by calcium binding, include three DGK isozymes (alpha, beta and gamma). DAG kinase alpha, also called 80 kDa DAG kinase, or diglyceride kinase alpha (DGK-alpha), is active upon cell stimulation, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DAG kinase beta, also called 90 kDa DAG kinase, or diglyceride kinase beta (DGK-beta), exhibits high phosphorylation activity for long-chain diacylglycerols. DAG kinase gamma, also called diglyceride kinase gamma (DGK-gamma), reverses the normal flow of glycerolipid biosynthesis by phosphorylating diacylglycerol back to phosphatidic acid. Members of this family contain two copies of the C1 domain. This model corresponds to the second one. DGK-alpha contains atypical C1 domains, while DGK-beta and DGK-gamma contain typical C1 domains that bind DAG and phorbol esters. The C1 domain is a cysteine-rich zinc binding domain that does not bind DNA nor possess structural similarity to conventional zinc finger domains; it contains two separate Zn(2+)-binding sites.
PSSM-Id: 410401
Aligned: 38 rows
Threshold Bit Score: 75.4636
Created: 1-Nov-2019
Updated: 25-Oct-2021
Aligned Rows:
Zn binding site
Feature 1: Zn binding site [ion binding site], 8 residue positions
Conserved feature residue pattern:H C C C C H C CClick to see conserved feature residue pattern help
  • Comment:Two non-consecutive sets of zinc-binding residues form two separate metal-binding sites.
  • Comment:Based on the structure evidence that Homo sapiens DGKdelta binds two Zn2+ ions

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
Feature 1         #           #  #              #  #    #  #        #   
Q01583        450 HHWVEGNCy-GRCSKCRKRIKayhgiTGLTCRWCHMMLHnrCASSVKk-eCTLG 501  fruit fly
ELT98962      311 HHWVEGNCp-GKCDRCKKSIKtyngvSGLHCRWCKITLHnkCASQVKp-eCDFG 362  Capitella teleta
XP_013400270  298 HHWVEGNCp-GKCDRCKKSIKtyngvTGLHCRWCQLNLHnkCASQVKp-dCDMG 349  Lingula anatina
PIK51210        3 HHWVEGNSp-GKCDRCKKSVKtynsvTGLRCRWCKMTLHnkCATNVKp-eCNMG 54   Japanese sea cucumber
RMX46771      177 HHWTEGNCp-GKCFKCGKAVKsnnclTGLRCAWCQVTVHnkCATHMAp-eCNLG 228  Pocillopora damicornis
PAA77405      245 HHWTEGNCv-EKCAKCRKSIKsg--iTGMQCRWCKVSLHnkCAEQWTs-eCDLG 294  Macrostomum lignano
PAA60718      207 HHWTEGNCagARCGRCRRTLGrs--lANLRCRWCQVCLHgrCAEQWAdqaCDLG 258  Macrostomum lignano
XP_009019307  205 HHWVAGNCs-AKCNKCKKHIKsynklTGKRCRWCKISVHnkCLELVGa-eCDMG 256  Helobdella robusta
Q03603        330 HHWQDANAt-AKCVKCKATVGv---fQGKGCRWCHNYVHhrCMSALAq-eCDLG 378  Caenorhabditis elegans

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