5J4A


Conserved Protein Domain Family
CDI_toxin_BpE479_tRNase-like

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cd20726: CDI_toxin_BpE479_tRNase-like 
C-terminal (CT) toxin domain of the contact-dependent growth inhibition (CDI) system (CdiA-CT) of Burkholderia pseudomallei, and similar proteins
CDI toxins are expressed by gram-negative bacteria as part of a mechanism to inhibit the growth of neighboring cells. This model represents the C-terminal (CT) toxin domain of CdiA effector proteins. CdiA secretion is dependent on the outer membrane protein CdiB. Upon binding to a receptor on the surface of target bacteria, the CDI toxin is delivered via the C-terminal domain. A wide variety of C-terminal toxin domains appear to exist; this particular model contains the C-terminal (CT) domain of Burkholderia pseudomallei E479 CdiA. This CdiA-CT domain is a tRNAse that contains a core alpha/beta-fold that is characteristic of PD(D/E)XK superfamily nucleases. It is structurally similar to another CDI toxin domain from B. pseudomallei 1026b which is unrelated in sequence but has a similar nuclease domain, and shares similar fold and active-site architecture. The PD(D/E)XK superfamily includes most restriction endonucleases and other enzymes involved in DNA recombination and repair.
Statistics
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PSSM-Id: 412043
Aligned: 56 rows
Threshold Bit Score: 67.3448
Created: 24-Sep-2019
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:inhibitor binding site [polypeptide binding site]
Evidence:
  • Comment:CdiA protein binds neighboring bacteria and delivers C-terminal toxin domain (CdiA-CT) to inhibit target-cell growth; CdiI immunity protein specifically neutralizes cognate CdiA-CT toxin to prevent self-inhibition
  • Structure:5J4A: Burkholderia pseudomallei C-terminal domain of CdiA toxin (CdiA-CT) binds CdiI immunity protein; contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                           #                                  ##                  ###    
5J4A_A         39 DPAVGKf--------rPGEAGAAAELENyl--ggTLQRAPqg---------ssVDFVFSsgp-----nnGKTVDFMLTpd 94   Burkholderia ...
ABQ90460        4 EPRGGRy--------rPHEGEVGAIIEDwf---gGLRRSPdp----------eADWIGTsgs-----yeGKTFDLIGLpr 57   Roseiflexus s...
WP_146393710  323 NNSSVGk--------aLVEAATRRRAQSq-----LGRRLTdsa-------dpaFDYVDDl---------DRTYDQMGN-- 371  Planctomycete...
WP_100902181 2417 PKATGGttpatpvappPALAQARSNLLQtl--rqRLLSYDpkrq-----qfipSEFKIAgime--srfgPVSRDSSGAtd 2487 Nostoc flagel...
SFF91940      468 DAGRGFr---------TDEAASATLFEEqs--gvRLGRYDtpdpvpgqptppnPDWVGAd---------GKTYDAMGR-- 525  Streptomyces ...
EWM12988      840 DPAKGWkf-------nPDEQAMALELEQkf--gvQLVRWPna---------qgPDWIALd---------GRTFDGIGP-- 890  Kutzneria sp....
SUE30007      219 DDSVKSl--------rAHEQATVARLAEdprfagRTFKAPppp-------dpgYDWYDDl---------GRHYDALGD-- 272  Rhodococcus r...
WP_149432789  301 DPNFGRl--------rPAEQATLRRAQEahp-elELRAVEne---------rdGEYEDKd---------GNTYDQMGN-- 351  Rhodococcus s...
OQP63237     2980 DPLRGDgvk-----lsEFEAESGALLEEay--diKLRELKpg---------eiGDYAVEsgkinginvaGKTIDQTGVp- 3042 Niastella vici
SEE04653     1115 IADMGKnrs-----lsASEAETLNRLNA------DPRFADrglkps--kaerdGEYVDNa---------GRTYDQMGD-- 1170 Amycolatopsis...
Feature 1                                  ## ##  ##                                            
5J4A_A         95 -------tvAQAAKINQFfdk-nlNNFMNTLSDHAAa---aDFVPLASRFlseaNKTLLVKAIGNlpqKLQAKIILIK 161  Burkholderia ps...
ABQ90460       58 --------gASAFHSDNM------ENFLPAVDMHFLks--vDYIVLDVRFmtpaQKETVLRHINAqwaSEKSRLILVE 119  Roseiflexus sp....
WP_146393710  372 ---------PAMIPHWASq----rSQFFNQIDRHLQk---aDFTVIDLTSfpkqIQDDVANYVLNlpeHLFDKVIPIG 433  Planctomycetes ...
WP_100902181 2488 witggrrtiDAVGPVPSEhf--nlESFTTQITKHLSk---tDIVPIDLSGltsgQINDVQNFLKGlsaPQRKQIIIIP 2560 Nostoc flagelli...
SFF91940      526 ---------NLTPQRFADhwagngGDLKATIAKHVNk---aDVTIFDVTNlgasDIATLRAYYQEm--GWSSRVAFVG 589  Streptomyces alni
EWM12988      891 ---------FKSRYFDQEwa---kGGVQDSIIKHVMtk--aDMVPIKLGD----LTPEQAAKVRDfivPYQPYVFILE 950  Kutzneria sp. 744
SUE30007      273 ---------GSTSQYFKL------DEFTNSIDKHLLks--nDFTVIDMTGytpqQISDVSNYISSlpsESQTKIIKVG 333  Rhodococcus rho...
WP_149432789  352 ---------PATSEHWSPrv---eQSFEDSVQWHLLks--nDGTMIDLTGfseeSKTAIKEYLDSlpsEQQEKILRIG 415  Rhodococcus sp....
OQP63237     3043 ---------LSGVGKFKM------EKFYAQIKRHLRekqgaYFILIDLRHfseqERAEVLKHLSDnfsNDASRIITIQ 3105 Niastella vici
SEE04653     1171 ---------PRASLYWNP------GQFTRAIRDHLNks--mDYTVVDLTGfkpeQIAQVNEFLRTlppGELAKIIKIG 1231 Amycolatopsis l...

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