5J5V,5J43


Conserved Protein Domain Family
CdiA-CT_Ec_tRNase

?
cd20700: CdiA-CT_Ec_tRNase 
C-terminal (CT) domain of the contact-dependent growth inhibition (CDI) system protein CdiA (CdiA-CT) of Escherichia coli 563, and similar proteins
CDI toxins are expressed by gram-negative bacteria as part of a mechanism to inhibit the growth of neighboring cells. CdiA secretion is dependent on the outer membrane protein CdiB. Upon binding to a receptor on the surface of target bacteria, the CDI toxin is delivered via the C-terminal domain. A wide variety of C-terminal toxin domains appear to exist; this particular model contains the C-terminal (CT) domain Escherichia coli 563 CdiA and similar domains. This CdiA-CT (EC536) region is composed of two domains that have distinct functions during CDI. This domain is the extreme C-terminal domain, an RNase toxin that possesses an all alpha-helical fold and conserved aspartate and glutamate residues, and K[DE] and [DN]HxxE motifs. The N-terminal domain facilitates translocation of the tethered nuclease into the cytosol of target bacteria. Although this CdiA-CT rapidly cleaves tRNA in vivo, the purified toxin has no detectable nuclease activity in vitro. Experiments show that it is activated when bound to the biosynthetic enzyme O-acetylserine sulfhydrylase-A (CysK), which is one of two isoenzymes (along with CysM) that catalyze the final reaction in cysteine synthesis. CDI(+) bacteria also produce a CDI immunity protein (CdiI) to specifically neutralize the CdiA-CT toxins to prevent auto-inhibition. This CdiA-CT binds its cognate CdiI with high affinity.
Statistics
?
PSSM-Id: 411007
Aligned: 21 rows
Threshold Bit Score: 71.7353
Created: 29-Jul-2016
Updated: 25-Oct-2021
Structure
?
Program:
Drawing:
Aligned Rows:
 
inhibitoractivator
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:inhibitor binding site [polypeptide binding site]
Evidence:
  • Comment:CdiA protein binds neighboring bacteria and delivers C-terminal toxin domain (CdiA-CT) to inhibit target-cell growth; CdiI immunity protein specifically neutralizes cognate CdiA-CT toxin to prevent self-inhibition
  • Structure:5J5V: Escherichia coli C-terminal domain of CdiA toxin (CdiA-CT) binds CdiI immunity protein; contacts at 4.0A

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                        #   #        ##              ### 
5J5V_B         97 LTDAEKVELGgsgsgtgtpppsendpkqqnektvdklnqkQESAIKKIDNTIKNAl--kDHDIIGTLKDXd--gKPVPKe 172  Escherichia c...
WP_007771706  148 IENRIANTLNekhyda------------------vraeleGNVVATKPNGTPYDHitevREARAGQIDAA----KNIQTa 205  Rhizobium sp....
WP_112420529 1977 IDNRIEDALNerhfdav-----------------rreqngEVVKINPRDGEPYDHitevQNALNGQKKDI----QNLKTa 2035 Rhizobiales b...
KQU79355      562 IDSRINDPLNewhydav-----------------rrekkgEIVATNPRTGKPYDHvkevEDALHGQIRDI----ENIKTa 620  Ensifer sp. R...
WP_051966807 1156 ISEEAQQAITkyhnlmrdpigeanaqaghnhysaarqeaaGIVVARKKDGRPYSH----ISDLQQARDGM----KGVAKt 1227 Kitasatospora...
WP_121230201  893 LENLKKNPVGeinrepnhnh-----------ysaarrearGEVVARKANGRPFSH----IRDLQEACDGLfnvrETLSRe 957  Saccharothrix...
OKI19345     1477 LSDRAKDAIEkfhnlmkdpigeingtpnhnhynaarreaaGEVVARKPDGTPFSH----ISDLQQARDGL----LNVKKa 1548 Streptomyces ...
CDX21286     1846 IDNRIRNTLNeehyhav-----------------reelagKVVKINPKDDEPYDHitevRQNLAGQIKDI----NRLKDe 1904 Mesorhizobium...
CDZ81089      163 IEMSGRVLLDe----------------------------dGNPVINPKDGEPYEH----IKDVEGYQNAA----KKLKYa 206  Candidatus Ru...
WP_044882785  155 IKDAYKRLINltengalnryhyta--yrkeaqgivllnreGKPLINRKNGKPYDH----IKDVEGYQEGG----KNLIRd 224  Neochlamydia ...
Feature 1                ###      ##  #  ##  ##                                 
5J5V_B        173 ---nGGYWDH-----XQEXQNTLRGLRNHADTLknv----nnpEAQAAYGRATDAINKIESA 222  Escherichia coli 536
WP_007771706  206 ---lGSPSLN-----PAARETLEQALSKASKSIdeatkalkgeNTQTTHDKSSTRGDDKSSS 259  Rhizobium sp. CF080
WP_112420529 2036 ---lGSPSLN-----EKARRDLEDALSRASKSAd---------LASRALKGEGDSRHDGSAS 2080 Rhizobiales bacterium
KQU79355      621 ---lGSPSLS-----KEAQKELQDVLSRASKNAdaass-alwgERPTTHDKGRNDREQKGAG 673  Ensifer sp. Root31
WP_051966807 1228 ----LGRQLIkggdlSEEDRSMLAGYVRASNRQagrl---ssfLHGIGHGTTPPYHEWPPGS 1282 Kitasatospora mediocidica
WP_121230201  958 ---aHNPPDTm---tERGMHVLLERLSEVNRLInrl----agfLNEIGHGSFPPYHEWPPGS 1009 Saccharothrix variisporea
OKI19345     1549 l--hDEFRFN-----KELTDRDVQILKKYQAATegqlgrlnrfLHGIGHGTTPPYHAWPPGS 1603 Streptomyces sp. CB03911
CDX21286     1905 ldqtKHPNLD-----PRAKKSMEDALSRASKSAdaakkaldgkTTQQTHDKTSDSANSKPGK 1961 Mesorhizobium plurifarium
CDZ81089      207 l-qsKLASNSi---tKEERDELQKLLSKVCKVLdds----krfTTSADRSKTPSKQEFNQLL 260  Candidatus Rubidus massiliensis
WP_044882785  225 i-rlRLRFDDl---tADERKELESILAKTSRILd--------dSYKFTTKAATPAHKQFYQT 274  Neochlamydia sp. EPS4

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap