4NTQ


Conserved Protein Domain Family
CdiI_ECL-like

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cd20699: CdiI_ECL-like 
inhibitor (or immunity protein) of the contact-dependent growth inhibition (CDI) system of Enterobacter cloacae, and similar proteins
CDI toxins are expressed by gram-negative bacteria as part of a mechanism to inhibit the growth of neighboring cells. This model represents the inhibitor (CdiI, also called CdiI immunity protein) of the CdiA effector protein from Enterobacter cloacae, and similar proteins. CdiA secretion is dependent on the outer membrane protein CdiB. Upon binding to a receptor on the surface of target bacteria, the CDI toxin is delivered via its C-terminal domain (CdiA-CT). The CdiI immunity proteins are intracellular proteins that inactivate the toxin/effector protein to prevent auto-inhibition. They are specific for their cognate CdiA-CT and do not protect cells from the toxins of other CDI+ bacteria. Thus, CDI systems encode a complex network of toxin-immunity protein pairs that are deployed for intercellular competition. Although E. cloacae CdiA-CT has structural homology to the C-terminal nuclease domain of colicin E3, which cleaves 16S ribosomal RNA to disrupt protein synthesis, and has been shown to use the same nuclease activity to inhibit bacterial growth, the corresponding CdiI immunity proteins are unrelated in sequence, structure and toxin-binding sites. Structural homology searches reveal that E. cloacae CdiI is most similar to the Whirly family of single-stranded DNA-binding proteins.
Links
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Statistics
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PSSM-Id: 412051
Aligned: 54 rows
Threshold Bit Score: 141.562
Created: 30-Sep-2020
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heterodimer
Conserved site includes 24 residues -Click on image for an interactive view with Cn3D
Feature 1:heterodimer interface [polypeptide binding site]
Evidence:
  • Comment:CdiA protein binds neighboring bacteria and delivers C-terminal toxin domain (CdiA-CT) to inhibit target-cell growth; CdiI immunity protein specifically neutralizes cognate CdiA-CT toxin to prevent self-inhibition
  • Structure:4NTQ: Enterobacter cloacae CdiI immunity protein binds C-terminal toxin domain (CdiA-CT); contacts at 4.0A
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                       #            # ## #                                    #     ## #
4NTQ_B         2 FGIFSKGEPVsx-egELVQPSSIVINd-YEEELHLPLSYWDIKDYKNSWLKSLGEGLsn-kTHSALAVSXYEpektNFIF 78  Enterobacter cl...
WP_136527887   1 MLTITASTLDaa--aNGYFTGCLRFPdgEVEYFRSSLGHWSVERYAASWRAQFAALRrg-aSVAVLFTGVEDprsaNFVT 77  Glycomyces paridis
AKT43573       4 FDIYFLDDFYva-gmEGSYFGEICIGs-FREKFALDSLFWSRDRYEQQWIEAARRIMt--hDRAVMMASISDpataNFFR 79  Chondromyces cr...
WP_130468161   2 FNIYLTDESDel-lgENVYKGHIVAGe-LNEYFRSEVNWWSKDDYKRSWLREIIRFLegetDVAVLITTFVPpenaNFLR 79  Actinopolyspora...
WP_147647934   3 FSISFRDEPDvideeLRLAHGDLVIGt-FHEELYANLFELSRADYEALWKLALETVLs--gQKAALIVYYVSpeqsSNME 79  Terriglobus alb...
WP_153341547   3 FAITLTGETAad--dPTLAIGALTLGh-HTENFRTPIGHWTATDYETSWSLALNRLVhg-aTISCLVTAMRDprkaHFLE 78  Nocardia sp. RB56
WP_146220819   2 FDLFFTSEGRmf--gEAGYNGSIKIGn-FSENFFSNNIFFNARQYQEQWLAAAERVLs--nGKSYFLVNVYDpdmaNFFT 76  Komagataeibacte...
SBW24406       2 FRIAFSDHHDra-ddFDGLVGKIVAGg-LDETFEAPIGKWTQSDYESSWRGELLSLLrg-eDVAVLLTVTIDpreaNWLR 78  Candidatus Fran...
WP_095349269   1 MFDLFLEKSDnf-ydEDGYWGKIIINd-FNERFFSNTTFFDKASYQKQWRHAAELLLk--kKKSYFLVSVGNpitsNFFM 76  Acetobacter fab...
WP_045053822   2 FAIEFVGQPCie-fnQPAIRGRITLGd-FSEEFVAPLVFWTVDDYQKQWREAAERIVag-eARSCFAASIREspddGAIF 78  Aliterella atla...
Feature 1         #             ### # #                    #  #  # ######            
4NTQ_B        79 TWVLYFEd-eKVYVQNNVIFLEechGFSPENINk---FIESRTTHDg-DGXKISEWHTDLNSVLDFYH 141 Enterobacter cloacae subsp....
WP_136527887  78 GWVVYRPgdgFLYLQERLFLDSqlpVGWELRDLg--rTVGERTTVTe-DGHAVAEWRVRLADIDPQRT 142 Glycomyces paridis
AKT43573      80 WWALYRDr-dLIAVQEHYCPLAeldRPFSLDRPe--eSMQPRSTRSe-DGVFISEWFTTVRAMQAFLE 143 Chondromyces crocatus
WP_130468161  80 GYVLYGFg-dDVLIQEQMFVVEslgYVFDPYSPg---DFLKYSSTTe-EGEKKSEWRMEKEDLRTFVS 142 Actinopolyspora sp. DSM 45956
WP_147647934  80 WWPMYPDg-dIVYFQNQMPWHDrfpEPFSLDAPf--aLLHNRRTISedTGDAISEWVVHRSAIEAFLS 144 Terriglobus albidus
WP_153341547  79 IWPLYREd-rMVHVQNQLLFLNeldREFDPTKPw--nFIEPHSTADe-DGQRISEWSVLTSHIEEFLK 142 Nocardia sp. RB56
WP_146220819  77 SWPCSVId-gTVYIQNQIIFADq-yCLSDILSFq--tNLGEIEFIDe-DGEKFSTWQTDLHAMQQFRT 139 Komagataeibacter nataicola
SBW24406      79 GYTLYRFg-dEVRIQERMFLMDeldHGFDLRNPs--nFASRYESVNe-NGRRISEWVVSLADIRRFLE 142 Candidatus Frankia californ...
WP_095349269  77 SWPCYLQg-dIVYLQNKMILAD---QHPLDKIIsneaDLGDLRFTNe-DDQKISTWETDIQSFVNFKD 139 Acetobacter fabarum
WP_045053822  79 IWTAYKLa-dTVHFQHKLLLPEtvkGTFEPLNIy--aQVDERQTKTe-DGFQISEWQVTVKDVAYWLR 142 Aliterella atlantica

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