3EEB,3GCD,6AR6


Conserved Protein Domain Family
Peptidase_C80

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cd20500: Peptidase_C80 
peptidase C80 family
The peptidase C80 family includes self-cleaving proteins that are precursors of bacterial toxins such as the Vibrio cholerae RTX self-cleaving toxin, as well as the major virulence factors of Clostridium difficile multidomain toxins, TcdA and TcdB. These toxins contain a cysteine protease domain (CPD) that autoproteolytically releases a cytotoxic effector domain upon binding intracellular inositol hexakisphosphate. This family also contains filamentous hemagglutinin family cysteine protease C80 domains, that are located at the C-terminus. All domains in this family contain the characteristic Cys/His residues in the active site. Site-directed mutagenesis has identified functional residues Asp/His/Cys in Clostridium toxin B and His/Cys in cholera RTX toxin.
Statistics
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PSSM-Id: 410973
Aligned: 99 rows
Threshold Bit Score: 87.3627
Created: 29-Mar-2017
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
catalytic siteallosteric
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1: catalytic site [active site], 2 residue positions
Conserved feature residue pattern:H CClick to see conserved feature residue pattern help
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                    #    
3EEB_A         32 GQIIVQMEnd--pVVAKAAANLAGKHae----sSVVVQLDs------dGNYRVVYgdpsk----ldgkLRWQLVGHgrdh 95   Vibrio cholerae
XP_028674990    6 HRVILVLGed--eVAVQAGKDYYEKEyhktgtkKTLLQWRd-------NAIHLVKstrqnh-ldvskdSKLVIIGHgsva 75   reedfish
OWQ44118      486 LRLVLQMEqd--sVIAESCATLHRRHad----rSVWIHIDd------gGRPTVVVgaallirvpsdvaLTMAIVGHggra 553  Mitsuaria noduli
XP_014353851  107 SHVIIVIGsd--eIVNLAGQRLFEKTqdssgakKILLQWNkn-----tETFSRISgstnv---elsdlSKLTIVGHgsre 176  coelacanth
RZI63019      188 LRLVVQLSpe--lDLLTYAAAQRVTYpd----nCVLIQANrhgvcrviEGRHLLHdwrk------ghpIKVEFFGHggrl 255  Rubrivivax sp.
ANH70989     1182 LRLHLQVEdd--rAMLRGARRAVRNHpn----eVVWIQVDr------lGNHEIRYgegllkqvtpdtpVKISIEGHgrtd 1249 Mitsuaria sp. 7
OCT93505       98 HEAILRIGtd--eTVRKAGEDLYNKQtsi-drnKCLLDWDes-----tGQFKVVKskgdgg-iditahDKLTVVGHgghr 168  African clawe...
ANH70989     1834 RRLLVHLQmeddiAMLNFARRTIRTHpd----eTVWIQVDr------eGGHVIRYgehllkqagaelpVKIEISGHgshd 1903 Mitsuaria sp. 7
ANH70989      484 LRIQLQMEdd-waSVVNIASALHRHPd-----nTVWIRAGs------dGSWHVVRgkellshlapdvnIAIEIVGHgtpv 551  Mitsuaria sp. 7
WP_067283748  440 LRLFLQMGp----DSPNLALERFRYPd-----eAVWIQIDr------hGHQRIVGggallsritpdtrVKLEIEGHdsls 504  Mitsuaria sp. 7
Feature 1                                                         #                               
3EEB_A         96 setn--ntrlsgysADELAVKLAKFqqsfnqae--ninnkpdHISIVGCslvsddkqkgFGHQFINAMDANgl---rVDV 168  Vibrio cholerae
XP_028674990   76 dei-----ringlnADELSKVILSLknqnsplh--frsksikMITLLSCgtgagnegwkFAEDFLIHLRENyl--evGRI 146  reedfish
OWQ44118      554 aegtgdrmllsdytPDALAQCVSRClgalgl------pmnvsRITLDSCmletptfdrgYAADFLRCVDDLgltavdATV 627  Mitsuaria noduli
XP_014353851  177 sei-----tlgelsSSDLAHVILRLyennsplh--fsskyikALSLVACnigskrkgdaFLRDFLVTLDNQgl--rtGSL 247  coelacanth
RZI63019      256 gtgd--rpslngytPETLAERLAQVmtslkl------qarvhAITLISCalaspafpghFAADFLIAARQRhllrptARV 327  Rubrivivax sp.
ANH70989     1250 dttr--tqrlgdstPSELLERVAPVierlgl------qsravEGVLLGCqlvalfatgdYAEDFLSEAMKRglfrqeASL 1321 Mitsuaria sp. 7
OCT93505      169 dgk----vsvggktSDQLAKAILSLrestapihwpsnmkaleEISIVSCevgageqgetFTKEFLLGLKEGgi--tvGSV 242  African clawe...
ANH70989     1904 patr--tqrlsgygHADLVERASAVigdlgl------msrarQLTLVSCaieappvadsFGRGFMETAIAKglvhedASM 1975 Mitsuaria sp. 7
ANH70989      552 qnar--rmqlsgysPVPLSQRVSALmadlgl------pakvtKVTLYSCaletlmaaerYSRTFLNSAIDMdwmippGKV 623  Mitsuaria sp. 7
WP_067283748  505 dtgt---telehyaPAALVDRVKSAlhelgi------trttsRVTLRSCalvsravpdsFAERFIEAGVDRglltgdAQV 575  Mitsuaria sp. 7
Feature 1                                   
3EEB_A        169 SVRSs--ELAVDe-------aGRKHT 185  Vibrio cholerae
XP_028674990  147 SAFKv--GIAVLp-------tGEKVT 163  reedfish
OWQ44118      628 TAYAh--TVQTW---------SRYRT 642  Mitsuaria noduli
XP_014353851  248 SARTa--DVTVRp-------dGRKET 264  coelacanth
RZI63019      328 TAYTervGMVVDrq------tGRFLS 347  Rubrivivax sp.
ANH70989     1322 EAFAe--NVVFNd--------AERRA 1337 Mitsuaria sp. 7
OCT93505      243 SVRTt--KTIVEe-------dGRKAT 259  African clawed frog
ANH70989     1976 VAYG---EVLVRrtglladaePSRTT 1998 Mitsuaria sp. 7
ANH70989      624 TGYA---EVVGShprmtqkygGAEWR 646  Mitsuaria sp. 7
WP_067283748  576 TAYS---EIMGF---------DLRRL 589  Mitsuaria sp. 7

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