Conserved Protein Domain Family

cd20496: C58 
peptidase C58 domain
The C58 family peptidases are endopeptidases that also act as transamidases, attaching a lipid moiety to the newly exposed N-terminus of the substrate. These include the Pseudomonas avirulence (Avr) protein AvrPphB and the homologous protein from Yersinia known as YopT; both are involved in bacterial pathogenesis. These proteins have a papain-like fold and a distinct substrate-binding site. The proteolytic activity of AvrPphB is essential for autoproteolytic cleavage of an AvrPphB precursor as well as for eliciting the hypersensitive response in plants. Yersinia pestis YopT cleaves the post-translationally modified Rho GTPases near their carboxyl termini, releasing them from the membrane. This leads to the disruption of actin cytoskeleton in host cells. Also included in this family is the Pseudomonas syringae HopN1 peptidase, a type III secretion system effector that can suppress plant cell death events in both compatible and incompatible interactions. All of these proteolytic activities are dependent upon the invariant C/H/D residues conserved in the C58/YopT family peptidase domain.
PSSM-Id: 410777
Aligned: 3 rows
Threshold Bit Score: 141.311
Created: 11-Jul-2019
Updated: 25-Oct-2021
Aligned Rows:
catalytic site
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: catalytic site [active site], 3 residue positions
Conserved feature residue pattern:C H [DE]Click to see conserved feature residue pattern help
  • Comment:these 3 catalytic residues form an active site triad

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
Feature 1                                       #                                             
1UKF_A      4 DFSVASRDVnh--------------------nNICAGLSTEWLVMSSdgdAESRMDHLdyn--------geGQSRGSERH 55  Pseudomonas savast...
EPM92639   47 LVHPFHQSKflfektiddrafaadygraggngHACLGLSVNWCQSRAkgqSDEAFFHKledyrgdallprvMGFQHIEQQ 126 Pseudomonas syring...
O68703    113 INFKFAQTKgaflhkii--------khsdtasGVCEALCAHWIRSHAqgqSLFDQLYVgg----------rKGKFQIDTL 174 Yersinia pestis
Feature 1                                                                                     
1UKF_A     56 Qvyndalraalsnddeapfftastav----------iedagfslrrepktvhasggsaqlgQTVAHDVaq-sGRKHLLSL 124 Pseudomonas savast...
EPM92639  127 Aysnkmqnaapmlldtlpklgm------------------tlgkglgraqhahyavalenlDRDLKAVlqpgKDQMLLFL 188 Pseudomonas syring...
O68703    175 YsikqlqidgckadvdqdevtldwfkkngisermierhcllrpvdvtgttesegldqllnaILDTHGIgy-gYKKIHLSG 253 Yersinia pestis
Feature 1            #               #                                                
1UKF_A    125 RfanvqGHAIACSCEg-sQFKLFDPNlGEFQSSRsaaPQLIKGLIDHYnslny--------dvacVNEFRVS 187 Pseudomonas savastanoi pv....
EPM92639  189 Sd----SHAMALHQDsqgCLHFFDPLfGVVQADSf--SNMSHFLADVFkrdvgthw-rgteqrlqLSEMVPR 253 Pseudomonas syringae pv. a...
O68703    254 Qm---sAHAIAAYVNeksGVTFFDPNfGEFHFSDk--EKFRKWFTNSFwgnsmyhyplgvgqrfrVLTFDSK 320 Yersinia pestis

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