Restriction endonuclease NotI and similar proteins
Restriction enzyme NotI is a type IIP restriction enzyme (the simplest being separate homodimeric endonucleases and methyltransferases that each recognize the same palindromic DNA target sequence) that recognize sites of 8 bp or longer in invasive DNA. NotI is commonly used for the introduction of radiolabeled landmarks in the restriction landmark genomic scanning (RLGS) method, which has become a common technique for the study of aberrant DNA methylation patterns in tumor- and tissue-specific cell lines.
Structure:3C25: Nocardia otitidiscaviarum restriction endonuclease NotI active site interacts with substrate DNA and divalent cations; contacts at 4A - View structure with Cn3D
Comment:The active site chemistry is similar to that observed in the BglII restriction enzyme with the arrangement F159D160/E182XQ184. Asp160 and Glu182 have been identified as catalytic residues by mutational analysis.