4P0P


Conserved Protein Domain Family
XPF_nuclease_EME

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cd20083: XPF_nuclease_EME 
XPF-like nuclease domain of crossover junction endonucleases, EME1, EME2 and similar proteins
The Mus81-EME1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. ERCC4 domain of Eme1 is a nuclease-like domain which is involved in targeting the MUS81-EME1 heterodimer complex to DNA.
Statistics
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PSSM-Id: 410859
Aligned: 31 rows
Threshold Bit Score: 131.637
Created: 18-Dec-2018
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
nucleic acidheterodimer
Conserved site includes 2 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleic acid substrate binding site [nucleic acid binding site]
Evidence:
  • Comment:DNA-binding induced conformational changes in the linkers connecting the nuclease and nuclease-like domain of Mus81 and Eme1 facilitating the DNA substrate bending and ultimately place the incision strand at an active site of Mus81.
  • Structure:4P0P; Homo sapiens Mus81-Eme1 complex binds flap DNA substrates; contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
4P0P_B        74 HIIVVLDPVLLqmegGGQLLGALQTm--eCRCVIEAqa-vpCSVTWRRRagpse--------dredWVEEPTVLVLLRaE 142 human
XP_013098154  28 YIRLVIDSRFMsasyGRQFIAELNNaagdLKYEVRNla-lkESIIWERSrgqltlaleqanaltedWQREKQVAKILTqE 106 stable fly
XP_017839271  28 YVRVILDVALRk---NSELLQQLSSt--dLKYDLRSlp-vsNCIIWERNvgqqtlst-igngledaWRLEGQILKVYSeA 100 Drosophila busckii
XP_023177770  28 YVRLVLDQGLLlipwGQELLEKLNNt--dLKYEIRSlp-arNCIFWERNvgqqtlva--gveledaWHMEQQVLLVYAeA 102 Drosophila hydei
XP_023291878  28 YVRLVVDSHILsapyGREFIAELNRaageLKYDIRTlp-ikESIIWERSmgqlalkpsevnalsevWQKEDQVVKILTqS 106 Australian shee...
KYQ46282     137 FMEVYLDRGIGsvssREEIESALQDa--gIKFNVTTei-ipNSITWRRNveedyisedndvrtrrsIQTEEYVIVIWSnY 213 Trachymyrmex ze...
XP_006617126 166 FMEVIIDKGIEnfsyITDIISTLLNt--nLQYSIKSqf-isNSITWKRNienhyvnenneictitnIENIDQILIIWNwD 242 giant honeybee
KOX80287     166 FIEVTVDKAIAnftsITDVTDTLRDa--eVKCTVKTkf-isNSITWKRNiensyvnddneictvtdAEKVSQILVIWNwD 242 Melipona quadri...
XP_018566241 174 YVIVEIDPDMFdneyGTILKEAFQKr--dINFRTKSqv-lpNLISWSRKiqtidd-----kvlresEVNESHFLLIIHaT 245 Asian longhorne...
XP_008548205 111 FIKVDFHNNISkfnfYDEIISMLDAs--sVDYSTKSslpapNSISWRRIveeysidhdnkvststsEQQEKFLLIIWTwD 188 Microplitis dem...
Feature 1                                                                                        
4P0P_B       143 AFVSMIDngkqgsldstmkgketLQGFVTDITAkta--gkALSLVIVDQEKCfsaqnpprrgkqgankqt--kkqqqrqp 218 human
XP_013098154 107 ELQQMIKkd-------------nLAQLPEELSTkc---pnMHHIVIYISNGQk--------------------------- 143 stable fly
XP_017839271 101 EFQHALKdq-------------sLTCIGSQLQTafpqpnsRYVIAVPRLHQSkq-------------------------- 141 Drosophila busckii
XP_023177770 103 DFQRALKdh-------------nLGCIRAKLHDafpalncQFTVVVPRLRQSk--------------------------- 142 Drosophila hydei
XP_023291878 107 ELVQMINts-------------tLHTLAESFKKhy---ekKQHIVLFLRSAEk--------------------------- 143 Australian shee...
KYQ46282     214 KAVKHVAeg-------------tFCTSVSDYKAlip--nyNMTLVICGMEEYfayrkkqksdqnsgskgs--rykgkgnq 276 Trachymyrmex ze...
XP_006617126 243 ETVIKVAnd-------------nFCTSISDIKSllp--dyKIILVIFGIENYfaykmqeknsdknetknkmqktniknnl 307 giant honeybee
KOX80287     243 EVATKITng-------------dFCTSVSSIKCslp--nyKITLVVYEKYLQlskhs----------------------- 284 Melipona quadri...
XP_018566241 246 EFMEHIKkd-------------tLTVYIGNVFSlte--ykQLTVVVVGLEKP---------------------------- 282 Asian longhorne...
XP_008548205 189 KVIQLIHdk-------------sFVSAVTNLYNsvr--emNLTLVIYGAEKYfeyheskgkkktsn----------kmye 243 Microplitis dem...
Feature 1                                                       #       #
4P0P_B       219 easigsmvsRVDAEEALVDLQLHt-eAQAQIVQs-WKELADFTCAFTKAVAEAPFK 272 human
XP_013098154 144 --------qSHSESAALIELQVMhqlHILQINSs-MKGLVEELKRLTKAIAEAPYK 190 stable fly
XP_017839271 142 -------raKGSVDTALIELQLSg-lHIEQLRPphTQELLALLQRYTKAIAEMPYK 189 Drosophila busckii
XP_023177770 143 ---------HSHETNALIELQLLqhlHVEQLAQpqCQEFILLLQRYTKAIAELPYK 189 Drosophila hydei
XP_023291878 144 --------qTTAISTALIELQILhqlKVLQVQPp-IRELLLQLKRLTKSIAEVPYK 190 Australian sheep blowfly
KYQ46282     277 qfdilpiisRQQLEMCLTEVQIIv-kCSSRLIEn-SQDLALMICQYTKSISEIPYK 330 Trachymyrmex zeteki
XP_006617126 308 eyknfveisRRQLEICLNEIQIIa-dCSSRLINn-SQDLALMIYQCTKAIAEIPYK 361 giant honeybee
KOX80287     285 -----cdisRKQLETHLNEIQIIa-dCSSRVINn-SQDLALMIYQYTKAIAEVPYK 333 Melipona quadrifasciata
XP_018566241 283 ---------KPPQEMPIIEVQMSt-kAYFRFAEn-AESLADILLEVTKAISQIPYK 327 Asian longhorned beetle
XP_008548205 244 ciddipkvsRNLFDANLIQIQILa-kCNSRLIEe-PADMSLLIYQFTKSIAEIPYK 297 Microplitis demolitor

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