XPF-like nuclease domain of crossover junction endonuclease EME2
EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA.
Comment:DNA-binding induced conformational changes in the linkers connecting the nuclease and nuclease-like domain of Mus81 and Eme1 facilitating the DNA substrate bending and ultimately place the incision strand at an active site of Mus81.
Comment:Based on the structure evidence that Homo sapiens Mus81-Eme1 complex binds flap DNA substrates