Conserved Protein Domain Family
XPF_nuclease_EME2
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cd20082: XPF_nuclease_EME2 
XPF-like nuclease domain of crossover junction endonuclease EME2
EME2 interacts with MUS81 to form a DNA structure-specific endonuclease which cleaves substrates such as 3'-flap structures. MUS81-EME2 is responsible for fork cleavage and restart in human cells. The MUS81-EME2 protein, whose actions are restricted to S phase, is also responsible for telomere maintenance in telomerase-negative ALT (Alternative Lengthening of Telomeres) cells. The nuclease domain of EME2 is a nuclease-like domain which is involved in targeting the MUS81-EME2 heterodimer complex to DNA.
Links
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Statistics
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PSSM-Id: 410858
Aligned: 13 rows
Threshold Bit Score: 229.138
Created: 15-Nov-2018
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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putativeputative
Feature 1:putative nucleic acid substrate binding site [nucleic acid binding site]
Evidence:
  • Comment:DNA-binding induced conformational changes in the linkers connecting the nuclease and nuclease-like domain of Mus81 and Eme1 facilitating the DNA substrate bending and ultimately place the incision strand at an active site of Mus81.
  • Comment:Based on the structure evidence that Homo sapiens Mus81-Eme1 complex binds flap DNA substrates
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
A4GXA9        68 RPEQVLKRLAVCVDTAILEDaGADVLMEALEALgCECRIEPQrpaRSLRWTRASPdpcprslppevwAAGEQELLLLLEP 147 human
POI25312       2 RPERCLRLLEVCVDPGFLEDpGSDIWIEALSSLdCKHSIEPQaipRSITWRRNMPsilsapddcmvkTEEEKEVLVLLEP 81  Chinese bamboo-...
XP_014345008 171 HPDQCVKYITVCIDPGLLKDvGSDTLMEALESMeLRYSIEPQsvsCSITWRREAPsnssi---tglnTVEEDQIVMLAMP 247 coelacanth
XP_023674903 146 RPENCLKRMTVCVDPVLLQDeGSDTLLGTLRAMeWRSSIEEQqlaHSITWNRELPeged-----glsIIQEEQILMVLQL 220 Paramormyrops k...
XP_015678474 144 RPDQCMKQMIICVDSGLLEHlGSDVLLEVLDSLdCKYYIESQripHSITWKRKIFsrtpnsdgcqeeTAVEKEVLLVMQP 223 Protobothrops m...
XP_006637087 175 RPEQCLKYLTVCIDPAVLQDeGSDLLLGTVEALgWRIIIEEQpvsHSISWKRDLPqvee----deasPVTEEQVLMVVTL 250 spotted gar
XP_026851669  94 QPENCMRSLTVRVHPVLLQEsGCDVLLACLDCLdCLSCIEDHaltHSISWRRRLSqd--------dtEVEEDQVLMVFSQ 165 electric eel
Q0IHN5       151 RPDQCGKYMVVKVDAGLLEDaGSEDVLEALRVAeYNYSIEPHsvpQSISWRREMPadwtciegmdmkEEEEDQLLVLVEP 230 tropical clawed...
XP_012692563  86 RPENYVKCLTVRIHPVLLQDgNSDVLSETLSELeWKSCIEPHsltHSITWKRSVPqeea-----edgEVEEEQVLMVISL 160 Atlantic herring
AWP17573     135 RPENCLKSLTVRIDPALLQQdGSDILLDSLAAFeWRFSIESQqlpHSITWTRDLPqfqqg--gggmgSVEEEQVVLLLGL 212 turbot
XP_016314214 120 KPENFIKSLTLQIHAALLRDaGCDVLLGTVDGLqWRNRIENQglpNSISWTRQALqtede---akngVIEEDQVLMVISH 196 Sinocyclocheilu...
XP_019738960 106 KPENCLRSLTVCVDSALLQQpGCDVLLGTLSSFqWRYSIRQQalaNSITWTRDLPqlte----gaagTVAEDQLVQVLSA 181 tiger tail seah...
Q56A04        62 RADQVLGRLVVCVDPAVLEDaGSDILMEALGTLgCECRIEPQhqaRSLQWNVVRPdpapsnvpleakAENEQEQLLLLEP 141 house mouse

Feature 1                                                                                        
A4GXA9       148 EEFLQGVAtl-------------------------------tqISGPTHWVPWispettaRPHLAVIGLDAYlwsrqhvs 196 human
POI25312      82 ADFLKRLYsltqtfvd-------------------assgsqpdLNQVLPLASLegs-stkTYSLAVVGLDAYrcnqeqng 141 Chinese bamboo-...
XP_014345008 248 NEFLQLVNsmkqepre-------------------lssggsseTLDMLAFSLPeql-pgkTATLVVLGLDVYqwcnghfg 307 coelacanth
XP_023674903 221 NEFMDMLSsikeiqqen------------------ikqvecksLFNKLSQYLNtya--gkIVTLAVIGLSSWalweek-- 278 Paramormyrops k...
XP_015678474 224 SYFLRQLSssvqnagpd------------------sqrsppdlTHLCPFHNLEdps--tvSYSVVVIGLDTYqwynqnhq 283 Protobothrops m...
XP_006637087 251 SDFLDMVVaikkgqdrt------------------geeappgsLYTQLCEFLEgdp--ekVVTLAVIGPNPNswtlrgre 310 spotted gar
XP_026851669 166 EEFIVMVTsikeslng--------------------esegvesLFQPLSEYLKrtr--gtVISLLVTGQSDQsggsknyd 223 electric eel
Q0IHN5       231 KSYLSSVRtyaqapyyf------------------cvgnemeeIPGSVFSIPAknp--hkKVTLVIIGLQEYrwcerlsr 290 tropical clawed...
XP_012692563 161 YEFMDVVQsvkqvlkgd------------------ssrheksgLLAALSDHLNtht--esVVTMLVMGSGQHrkrgswyh 220 Atlantic herring
AWP17573     213 TDFLDMVIcvkkhscctiqplqycillplqmldsegdetgmgsFLGPLFEVLNrda--kkDVTLLMTDSHPGqgtnvchv 290 turbot
XP_016314214 197 NEFEDMVMshktvtnqsap--------------igdvyeeaesLLQHLYKYLNkts--rkFVTILVISHQQRsgsgdedd 260 Sinocyclocheilu...
XP_019738960 182 SQFVDVVVavkklidsd------------------geeleghpVPGPLAEGFRlda--akVATLLVTESRPEsrraalae 241 tiger tail seah...
Q56A04       142 QEFLQGAAql-------------------------------tqITDPPCSIPWlspksltRSHLAVIGLDAYlwshqlss 190 house mouse

Feature 1                                                                              #       #
A4GXA9       197 rgtqqp---------------espkvagaevavsWPEVEEALVLLQLWaNLDVLLVAsWQELSRHVCAVTKALAQYPLK 260 human
POI25312     142 wqplspg------------kqsprsqpgpelfmtQQEILEAQVVLQLWgDIDVQLLDtWQEFGEHVSALTKAIARRPYK 208 Chinese bamboo-p...
XP_014345008 308 fkcwkeegswreqlsvcpqtqnrdpqscldpavtPMDIEEALVALQLQkNMGVLFMEtWQELGEYITLLTKSVAKRPFK 386 coelacanth
XP_023674903 279 ----------------------------rsgglrDLDLEEALVFLQLSrNTSVIFLEsWQDLADHACAVTKALSKRPMK 329 Paramormyrops ki...
XP_015678474 284 lpqelipg-----------lenetlqlgpeqsmtRHQIEEALVVLQLRgNIHVLFLEtWQELAQHVSAVTRAVAQRPYK 351 Protobothrops mu...
XP_006637087 311 rrd---------------------rsmgsdlalkELDIEEALVFLQLHrNISVMFLPsWDELCDHMCAVTRALAKRPFK 368 spotted gar
XP_026851669 224 d------------------------gprsqlgllDVDIEEVLVYLQLCrNVSVHFVFgWQEVTDHVCAITKALSKRPFK 278 electric eel
Q0IHN5       291 qiqrqslda---------aegrdsnkdqsatratRQQIQEALVFLQLHyNTEVLCLDtWKELGQHVCAVTKSIAQRPFR 360 tropical clawed ...
XP_012692563 221 sh----------------------gdlrsqlgmqDVEIEEVLVYLELYkSVCVHFLYnWKEVTDQVCTYTKALSKRPFK 277 Atlantic herring
AWP17573     291 ---------------------------qcdlgmgNLNVEEVLVYLQLCkNISVVFLNgWQEVTNHVCAVTKALSKRPFK 342 turbot
XP_016314214 261 --------------------------fdfhlnqhHLDTEGLLVHLQLYwNVSVNFLFgWQEVTDHVVAVTKALSKRPYK 313 Sinocyclocheilus...
XP_019738960 242 t-------------------------irsrcgggNADVEEVLVFLQLRkNVAVFFVEsWRDVTDHVCAVTKSLSKRPSK 295 tiger tail seahorse
Q56A04       191 qktwql---------------kkskeahargaisWAEVEEILVLLQLHaNLDVLLMAsWQELSQYVCAFTRALSQLPSK 254 house mouse

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