4PHZ,1YEW,3CHX,3RFR,4PHZ,4PHZ,6CXH


Conserved Protein Domain Family
pMMO-AMO_C

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cd19412: pMMO-AMO_C 
subunit C of particulate methane monooxygenase (pMMO, also known as membrane-bound MMO) from methanotrophic bacteria, and of ammonia monooxygenase (AMO) from ammonia-oxidizing bacteria, and related proteins
This family contains subunit C of particulate methane monooxygenase (pMMO; EC 1.14.18.3), an integral membrane metalloenzyme that catalyzes the conversion of methane to methanol. MMO is the first enzyme in the metabolic pathway of methanotrophic bacteria. It also contains subunit C of AMO (EC 1.14.99.39) from ammonia-oxidizing bacteria such as Nitrosomonas europaea (AmoC1-AmoC3). AMO catalyzes the conversion of ammonia to hydroxylamine. pMMO, along with soluble MMO (sMMO; EC 1.14.13.25), and the related enzyme AMO are the only known enzymes capable of methane hydroxylation. pMMO is composed of three subunits, PmoB (B or alpha), PmoA (A or beta), and PmoC (C or gamma), each containing membrane-spanning helices, with three copies each of the subunits forming a cylindrical A3B3C3 oligomer with a hole in the center. This subunit of pMMO has a metal-binding site that is exposed to the center of the pMMO oligomer, the metal being zinc or copper. Although biochemical and mutagenesis data indicate that the active site is located at the dicopper site in subunit B, the metal-binding site in this transmembrane subunit C may also be functionally relevant since all ligands are conserved and best enzymatic activity is obtained from intact pMMO containing all three subunits. Zinc inhibition studies of several respiratory complexes in Methylococcus capsulatus and Methylosinus trichosporium suggest that zinc might inhibit proton transfer in pMMO by either replacing active site copper ions or another copper site that is involved in reducing the active site. Nitrosomonas europaea AMO is composed of three subunits; AmoA, AmoB, and AmoC; it has two nearly identical copies of AmoC encoded by duplicate amoCAB operons and a more divergent AmoC encoded by a monocistronic amoC. The significantly shorter related C subunit of AMO from ammonia-oxidizing archaea are not included in this model.
Statistics
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PSSM-Id: 410990
Aligned: 109 rows
Threshold Bit Score: 155.013
Created: 19-Jul-2016
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
metal bindingoligomer
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: metal binding site [ion binding site], 3 residue positions
Conserved feature residue pattern:D H HClick to see conserved feature residue pattern help
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
4PHZ_K        25 WIGLVLLNVFYLIVRIYEQVFgwragl-dsfapEFQTYWMSILWTEIPLELVSGLGLAGYLWKTRdrnvd-avtprEEMR 102 Methylocystis s...
WP_018163235  40 PLAIAPVIAMALFWRTWQQLFafdkgl-dstmpGFNEYWLSIFGFNMFGLPLIAASIFAAIIITGikasatpltphEEMK 118 Smaragdicoccus ...
ORU90029      12 LIACATMFGMLLIIWWPSFMYlgdlfgyaqeesIQGVSLLDFFKAELLIMLVVGVVLLNYKKVRQvtd------gmGRVN 85  Cycloclasticus ...
ORU92139       8 LLTFFVLCCMLLLLWWPSFSTlgdlfgyaekaeYKGVSLLDFFKAELLVLVTVWGFLISYKQNRVdd-------gdRLVV 80  Cycloclasticus ...
ORU93378       8 PVVFAVLALVLVMIWGPIFSSfadlfsyaktalYNGVTLLDIFKAELLILCIVWLVLMSYSKSNDtvd------gdAYVR 81  Cycloclasticus ...
OUR86427       9 PVVFIILFLMLGVIWVPSFLSlgdlflyaekakYHNISMLSFFKAELVVIIVVWLVLISYSKKTErid------gdTYVR 82  Cycloclasticus ...
PCH83707       8 PIAFVVLFIMLGVIWWPSFSNlgdlfgyaatseYKDVSLLDFFKAELLILVTVWVVLISYRGKGVvvd------gnRHVK 81  Piscirickettsia...
PCI19682       8 PIAFVVLFLMLGVIWWPSYSNlgdlfgyaenadYKGVTLLHFFKAELLVLLIVWAYLMSYKKGNRttd------gnKYVR 81  Piscirickettsia...
PHS72614       7 PVSLVLLFVMLGFILWPNVTNlgdlfsfaknfdYKGIPLSQLFFSELVILLTVWLVLSTYKKGKSivy------gdEYMI 80  Cycloclasticus sp.
PHS72615       8 PLAVVFLFLMLGVIWWPSFSNlgdlfgyaakaeYKGVSLLNFFRAELVVLVTVWAVLISYKEQNDmvd------gnAYVT 81  Cycloclasticus sp.
Feature 1                                  #   #            #                                    
4PHZ_K       103 RLVVLVQWLVVYGIAIYWgASFFTEQDGTWHMtvirdtDFTPSHIIEFYMSYPIYSVIAVGAFFYAKTRIPyfa--hgyS 180 Methylocystis s...
WP_018163235 119 RHWRLMLGMAAFAIASFF-GAHFGEEDGAWHQvtlrdtAFTPSHVALFYGSFPMMFYVIFGVYLYGRTRLPhmwggkavP 197 Smaragdicoccus ...
ORU90029      86 RHLVLFVWVSGQVIVGFVaGGFLAHQDTPWYQavgeagGIMPAQAIILLVCYPAYLFFGGGAYIYTKTRLPkfl--egkK 163 Cycloclasticus ...
ORU92139      81 KHIILMVFIIGQVFMGFFaGGFLVHQDVSWYQvvhessEVMPSQAVILLICYPLYLFFGGGAYLYAKTRLPafl--rnkQ 158 Cycloclasticus ...
ORU93378      82 RHVILMMFVIGQVFVGFFvGGFWAHQDASEYQivrgadAVMPAQAIILLICYPLYLFFGGSAFIYAKTRLAifl--kgkE 159 Cycloclasticus ...
OUR86427      83 RHLILVMFYLGQVFVGSLaGGFLVHQDASWYEvlhnsnEVMPAQAIILLICYPMYLFWGGSAFLYAKTRLPhfv--knkE 160 Cycloclasticus ...
PCH83707      82 RHFILVLLVVAQVFIGFFaGGFLVHQDASWYQvihganEVMPAQAIILLICYPLYLFFGGSAFIYAKTRLPvfl--qdkQ 159 Piscirickettsia...
PCI19682      82 QHLILMMFVIGQVFMGFFaGGFLVHQDASWYQvihganEVMPSQAVILLICYPLYLFFGGGAYIYTRTRMPkfv--rhkE 159 Piscirickettsia...
PHS72614      81 RHCILLLFVTGQVFMGFFaGGIVSYQDVTSSQtahevsNVMESQAVIFMVCYPLYLFFGGTAFIYARTRLPefl--snkR 158 Cycloclasticus sp.
PHS72615      82 RFLILVMFVIGQVFMGFFaGGFLVHQDASWYQviheanEVMPSQAVILLVCYPLYLFFGGSAFIYAKTRLPkfl--kgkE 159 Cycloclasticus sp.
Feature 1                                                                     
4PHZ_K       181 LAFLIVAIGPFMIIPNVGLNEWGHTFWfmeelfvaPLHWGFVFFGWMALGVFGVVLQILMR 241 Methylocystis sp. ATCC 49242
WP_018163235 198 LGFAIVIGGTTLLVFQVAFNEFGHTYWqteevfsaPLHWPFVFFAWMLIGVAAIWLPTLLR 258 Smaragdicoccus niigatensis
ORU90029     164 IVYLILTFAPLVFLPYYKGGVSVAPNElle--fiyLVVCWLLSTAWLVVGVLYLVFKSAKE 222 Cycloclasticus sp. symbiont of Poe...
ORU92139     159 VAFLVLAFAPLAFLPHYNTDMMVFNSIvae--fayLAAYWLLSICWAVFGVMFLIIKSTQE 217 Cycloclasticus sp. symbiont of Poe...
ORU93378     160 LSFMVLTFTPWLLLPFNDASLLQASNDlve--lfyLAVYWILSTVWVVWGVGFLILRSAKE 218 Cycloclasticus sp. symbiont of Bat...
OUR86427     161 TSFMVLTFAPLAFLPYYDSNMMAGGVDame--lvyLAAYWILSISWIIWGVGFLILKSVQE 219 Cycloclasticus sp. 44_32_T64
PCH83707     160 VAFMVLTFAPLAFLPYYDSGLLDVKIDiie--lvyRAVYWLLSVAWVVLGVLPIILKAAPE 218 Piscirickettsiaceae bacterium
PCI19682     160 VAFMVLTFAPLAFLPYYDSSLMDVKRDiaq--ltyMATYWLLSVGWVGLGVIYIVIHSAKE 218 Piscirickettsiaceae bacterium
PHS72614     159 VPFMMLTFAPMAFLPYYDASIGDFNKSvfe--wlyRIDYWLLSIAWVVIGVFHIAVSASKE 217 Cycloclasticus sp.
PHS72615     160 FSFMVLTFAPLAFLPYYDSSFMQAKKDlfe--iayLSVYWLLSVGWVVIGVLYIILKASQG 218 Cycloclasticus sp.

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