3WCZ,5AZ0


Conserved Protein Domain Family
AKR_AKR2E1-5

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cd19116: AKR_AKR2E1-5 
Click on image for an interactive view with Cn3D
AKR2E family of aldo-keto reductase (AKR)
Bombyx mori 3-dehydroecdysone reductase is a founding member of aldo-keto reductase family 2 member E4 (AKR2E4). It is a NADP-dependent oxidoreductase with high 3-dehydroecdysone reductase activity. It may play a role in the regulation of molting and has lower activity with phenylglyoxal and isatin (in vitro). This family also includes 3-dehydroecdysone 3b-reductase from Spodoptera littoralis and Trichoplusia ni, DL-glyceraldehyde reductase from Drosophila melanogaster, aldo-keto reductase from Bombyx mori, which correspond to aldo-keto reductase family 2 member E1, E2, E3 and E5 (AKR2E1/2/3/5), respectively.
Statistics
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PSSM-Id: 381342
Aligned: 19 rows
Threshold Bit Score: 431.321
Created: 12-Mar-2018
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
Conserved Features/SitesClick to see Conserved Features Help
 
active sitecatalytic
Conserved site includes 36 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3WCZ: Bombyx mori aldo-keto reductase (AKR2E4) in complex with cofactor NADP

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          ###### #                          ##  ##                      
3WCZ_A         6 PCIQLNd--GNTIPIVALGTGRGTAKesds---idevRQAVYWAIEAGYRHIDTAAVYqDEEQVGQGIAEAia-ngLVTR 79  domestic silkworm
CAB41997      24 MLKMLN---DREMPAIALGTYLGFDKggavtskdkqlRNVVMQAIDLGYRHFDTAAIYnTEAEVGEAIRMKid-egVIKR 99  Spodoptera litt...
EAA14236       5 TCINLSdqhSIALPTIGLGTYSILGAd---------gKEAIRTAIDVGYRMFDTAVAYgNEAIVGEAIREKirecnNLTR 75  Anopheles gambi...
CRL07545       4 PVHYLNn--NLKIPALGAGTGKLKGEt---------gVQAIKDAIDAGYRLFDTAFLYgNEDIVGQAISEKit-egVVKR 71  Clunio marinus
BBD35022      31 PTLQLSs--GGNIPVVALGTWLGHRPkgkvvqvtndtEQAVIYALEAGYTHIDTAYKYgIEDQVGRALTKKfs--eGLKR 106 domestic silkworm
NP_001163844  39 PKVRLSs--GHEMPVLGFGTYKLRGYq---------cSAAVHCAIETGFRHFDTAYYYeNEKEIGEALRTQik-mgNISR 106 fruit fly
NP_647839      7 YYFKHNd--GTHIQGIGLGTFASTEGd---------cERAVLHAIDVGYRHIDTAYFYgNEAEVGAAVRKKia-egVIKR 74  fruit fly
NP_729808      5 PTVKLNn--GYEMPILGLGTYNSKDNe---------gEAAVKHAIDVGYRHIDTAYFYqNEAEVGKAIRDKia-egVVKR 72  fruit fly
NP_610235      6 PTIRLNn--GREMPTLGLGTWKSFESd---------aYHSTRHALDVGYRHLDTAFVYeNEAEVGQAISEKia-egVVTR 73  fruit fly
NP_996068      5 PTVKLNn--GYEMPILGLGTYNLKKSr---------cEAAVCHALEMGYRHIDTAYLYrNEGIIGKVLAKLig-dqKLKR 72  fruit fly
Feature 1               #                                ##                                      
3WCZ_A        80 EELFVTTKLWNdkharDQVVPALQESLKKLgLDYIDLYLIHFPIatkpd-------------dspdNIDYLETWQGMQDA 146 domestic silkworm
CAB41997     100 EDVFLTTKLWNthhkrEQVAVAMKETLNKTgLDYVDLFLMHWPIalned-------------yshsNTDYLETWRATEEM 166 Spodoptera litt...
EAA14236      76 DDFFIISKLSGsyhrqDLVEKCCRMTLDRLgMDYVDLYLMHTPValqsekkcar--ngtklnaiddSIAPTEAWIGLEQC 153 Anopheles gambi...
CRL07545      72 DEIFIIGKLWGih--hDQVERACRETCQRLkVDNVDLYLLHFPVsftfhndse----kwpkdddvlDKDYMDVWVEMEKL 145 Clunio marinus
BBD35022     107 ESIFITTKLWNsyherEQVIPALRKSLENLnLEYVDLYLIHWPIamfen------------dtlldNVDFLDTWRGMIDA 174 domestic silkworm
NP_001163844 107 ENIFLTTKLWNthhdpRDVRRICEKQLELLgFSYIDLYLMHFPVgykyvcdeilm-pmsgdelqtvEIDYLDTWRAMENL 185 fruit fly
NP_647839     75 EDIFITTKLWCnfhepERVEYACRKTLKNIgLDYVDLYLIHWPFsykyrgdnelipkdangevelvDIDYLDTWGAMEKL 154 fruit fly
NP_729808     73 EDIFLVTKLWNifhdpERVEGICRKQLSNFgLDYIDLYLMHMPVgykyvddntllpkneddvlqlsDVDYLDTYKAMEKL 152 fruit fly
NP_610235     74 EEVFVTTKLGGihhdpALVERACRLSLSNLgLEYVDLYLMHMPVgqkfhndsn-----vhgtleltDVDYLDTWREMEKL 148 fruit fly
NP_996068     73 EQVFLVTKLWDiyhepKMVKYACDMQLKLLgVDYIDLYLMHSPVgvdyisdedlm-phengqlrtnDVDYVDTYRSMEQL 151 fruit fly
Feature 1                   ##                    #                         #####  # #           
3WCZ_A       147 RQLGLARSIGVSNFNaTQITRLVSNsyIRPVINQIEVNPTNtQEPLVAHCQSLgIAVMAYSPFGFVVSRgqtga-ppprS 225 domestic silkworm
CAB41997     167 VKLGYTKSIGLSNFNkLQVATVLQEctIKPVALQIEVHPQIiQEDLITYAKDEgIIVMGYSPFGSLVKRfgmdl-pgpkM 245 Spodoptera litt...
EAA14236     154 YQEGLCRSIGVSNFNeHQINALLSDasIVPAVNQIECSIGFnQRPMRKFCQQQnILVMGYTPLGKQKLPf---------L 224 Anopheles gambi...
CRL07545     146 VHLGLTKNIGVSNFNeKQLERVYRLsnLKPKCHEIEFHPGFtRYSLLKLCRELnISVIAYCPLGRHKLEkqe----pkfL 221 Clunio marinus
BBD35022     175 KKLGLTKSIGISNFNqEQIQRIIDSglEKPSALQIELNLNLqQPELFKFCRENdIVVMAYTPFGSLFPSkaqpdappprI 254 domestic silkworm
NP_001163844 186 VKLGMVRSIGLSNFNmEQIQRIIQCssSKPVVNQVEIWPGFlQKDLVDYCRYNgIIVTAFSPLGQPNRKnhc----pvyF 261 fruit fly
NP_647839    155 VDLGLTKSIGVSNFNeEQLTRLLANckIKPIHNQIEVHPALdQKKLIALCKKNgILVTAFSPLGRHNAElrt----ptfM 230 fruit fly
NP_729808    153 VKLGLVRSIGVSNFNsEQLARVLANceIKPVTNQVECSPALnQKALTAFCKKNdVTLTGYTPLGKPKPDiqk----pdfI 228 fruit fly
NP_610235    149 VDLGLTRSIGLSNFNaAQTERVLANcrIRPVVNQVECHPGFqQRQLREHAKRHgLVICAYCPLARPQPArqw----ppfL 224 fruit fly
NP_996068    152 VHLGLVRSLGLSNFNaNQLKRLLENcqIKPANLQIECHPELvQVPLIELCKFHnITVVAYSPLGRPKACnpl----pdyY 227 fruit fly
Feature 1                      # #              ######  #  ##                         #
3WCZ_A       226 DDPTLTALANKYRKSVGQILLRYLIDrGLIPIPKSTNKQRIAQNIDLFDFQLTFEEVAAINQFNKNHRVI 295 domestic silkworm
CAB41997     246 DDPVLTSLAKKYEKTPAQIVLRWLVDrKVVPIPKTVSPKRLLENINIFDFKLKEEEIEKINQFNSNTRYT 315 Spodoptera littoralis
EAA14236     225 QNNRLKEIALSVDKTTAQVSLRYLIDeGVVPIVKSTDRKRQQENLDIFDFKLTKQQLEELDAIGGDQRAC 294 Anopheles gambiae str. PEST
CRL07545     222 YDSKVQEIAEKYNKTTAQVALRFSVQsNVIPIPKSATKSRLKENIDVFNFQLTEDEVSYLKNFHDEANQI 291 Clunio marinus
BBD35022     255 DDERLVSIAQKYNKTVPQVALRFLIElGAVVLPKSVHKERIEQNIQLFDFSLTPEEFNLLKSFDTGYRTL 324 domestic silkworm
NP_001163844 262 FSEGMKRLVKKYKRSASQIVLRYLIDyGVVPIPKAANPIHIKENLNIFDFKLDEADTRLLRGIKPKSRIV 331 fruit fly
NP_647839    231 YDGKVQAIADKYNKSIAQVVIRYVIElGTIPLPKSSNPKRIEENFNVFDFKLDAEDHAILDSYHNGERVA 300 fruit fly
NP_729808    229 YSPEVAVIAKKYGKTTPQIVLRYLVGlGVIPIPKSSNTNRISENFDIFDFELTAEEMAVLDGYHTGERVV 298 fruit fly
NP_610235    225 YDEHAQNLAKKYGRTTAQICLRYLVQlGVVPLPKSSNKARIEENFRVFDFELSPDDVAGMEQYHTGQRTV 294 fruit fly
NP_996068    228 TDSKLLALAAKYGKTPAQIILRYLIDiGTVPIPKAAQRAHLVENLDIFDFHLTPEELLQMKTFNTGRRIW 297 fruit fly

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