Conserved Protein Domain Family
mRING-C3HGC3_RFWD3
?
cd16450: mRING-C3HGC3_RFWD3 
Modified RING finger, C3HGC3-type, found in RING finger and WD repeat domain-containing protein 3 (RFWD3) and similar proteins
RFWD3, also known as RING finger protein 201 (RNF201) or FLJ10520, is an E3 ubiquitin-protein ligase that forms a complex with Mdm2 and p53 to synergistically ubiquitinate p53 and acts as a positive regulator of p53 stability in response to DNA damage. It is phosphorylated by checkpoint kinase ATM/ATR and the phosphorylation mutant fails to stimulate p53 ubiquitination. RFWD3 also functions as a novel replication protein A (RPA)-associated protein involved in DNA replication checkpoint control. RFWD3 contains an N-terminal SQ-rich region followed by a RING finger domain that exhibits robust E3 ubiquitin ligase activity toward p53, a coiled-coil domain and three WD40 repeats in the C-terminus, the latter two of which may be responsible for protein-protein interaction. The RING finger in this family is a modified C3HGC3-type RING finger, but not a canonical C3H2C3-type RING-H2 finger or C3HC4-type RING-HC finger.
Links
?
Statistics
?
PSSM-Id: 438114
Aligned: 40 rows
Threshold Bit Score: 95.7598
Created: 6-May-2013
Updated: 17-Oct-2022
Structure
?
Aligned Rows:
Download Cn3D
 
putative Zn
Feature 1: putative Zn binding site [ion binding site], 8 residue positions
Conserved feature residue pattern:C C C H G C C CClick to see conserved feature residue pattern help
Evidence:
  • Comment:modified RING finger (C3HGC3-type)
  • Comment:The RING fingers found in RFWD3 and its homologs are modified as C3HGC3-type RING finger, rather than canonical C3H2C3-type RING-H2 finger, or C3HC4-type RING-HC finger.
  • Comment:consensus of the canonical RING-HC/RING-H2 fingers: C-X2-C-X(9-39)-C-X(1-3)-C/H-X(2-3)-H-X2-C-X(4-48)-C-X2-C, where X is any amino acid and the number of X residues varies in different fingers
  • Comment:A RING finger typically binds two zinc atoms, with its Cys and/or His side chains in a unique "cross-brace" arrangement.
Scroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1            #  #                  # #  #  #               #  #             
Q6PCD5       283 EGDTCTICLEQWTNAGd--HRLSALRCGHLFGYRCISTWLKgq----vrKCPQCNKKARHSDIVVLY 343 human
ABR17109      87 ATSSCSICMEPWTSGGn--HRISCLPCGHLFGRPCIIKWIRqrgg-nigKCPQCNKMCKVKDIRTLY 150 Sitka spruce
XP_003059614  93 GALSCSICLEPCALDGa--HQVSALSCGHCFGHACISKWLTrhkkgnggKCPQCNRRAKVADVRKLF 157 Micromonas pusilla CCMP1545
XP_002674044 366 PNNICSICLSAWSSTGl--HQVCCLSCGHLFGKNCIEKWLKkn-----sTCPKCKKSADRSHIRLLY 425 Naegleria gruberi strain NEG-M
NP_195965    116 EGLSCSICMEVWTSGGq--HQVCCLPCGHLYGYSCINKWFQqrr--sggKCPLCNKICSLRDVRKIY 178 thale cress
XP_004338878  33 SASQCTICLDSWQSSGp--HKICSLKCGHLFGRSCIEKWLRnn-----kLCPMCKAKAQRSHIRPLF 92  Acanthamoeba castellanii str...
NP_001132877 107 HTANCPVCMDPWTSQGp--HRISCIPCGHVYGRSCLEKWLTqrgn-rsaTCPQCGKRFKHKDIINLY 170 Zea mays
XP_001316667   5 EEENCMICMGPLATEGe--HRVCSLKCGHIFGYKCIVEWLNtk-----sTCPACNSPATVESIIPLY 64  Trichomonas vaginalis G3
XP_002674707 112 KDNICLICQDEWKTESnhpHKVVALNCGHIFGHSCISQWLQtk-----rICPVCKRPAEESDLRPIF 173 Naegleria gruberi strain NEG-M
OHT08107       9 DHGKCLICLDQMSRSGe--HQICSLPCGHLFGYCCIKQWLDen----qkCCPTCQKYAKSSMLRLIV 69

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap