1BC2,2YZ3,1ZNB,4WD6,3PG4,3L6N,1M2X,4EY2,1DD6,1A8T,2M5D,2YNT


Conserved Protein Domain Family
MBL-B1
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cd16285: MBL-B1 
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metallo-beta-lactamases, subclass B1; MBL-fold metallo-hydrolase domain
MBLs (class B of the Ambler beta-lactamase classification) are a diverse group of metallo-enzymes that are capable of catalyzing the hydrolysis of a wide range of beta-lactam antibiotics. MBLs have been divided into three subclasses B1, B2 and B3, based on sequence/structural relationships and substrates, with the B1 and B2 MBLs being most closely related to each other. Subclass B1 enzymes are most active with two zinc ions bound in the active site, and have a broad-spectrum substrate profile. Includes chromosomally-encoded MBLs such as Bacillus cereus BcII, Bacteroides fragilis CcrA, and Elizabethkingia meningoseptica (Chryseobacterium meningosepticum) BlaB and acquired MBLs including IMP-1, VIM-1, VIM-2, GIM-1, NDM-1 and FIM-1.
Links
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Statistics
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PSSM-Id: 293843
Aligned: 18 rows
Threshold Bit Score: 282.635
Created: 24-Feb-2015
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2M5D: Bacillus cereus metallo-beta-lactamase BcII binds the broad spectrum inhibitor R-thiomandelic acid and two zinc ions
  • Structure:2YZ3: Pseudomonas aeruginosa VIM-2 metallo-beta-lactamase binds (S)-2-(mercaptomethyl)-phenylpentanoic acid (M5P) and two zinc ions
  • Structure:1A8T: Bacteroides fragilis CcrA metallo-beta-lactamase binds the biphenyl tetrazole class inhibitor L-159061 and two zinc ions
  • Citation:PMID 9545432
  • Structure:1DD6: Pseudomonas aeruginosa IMP-1 metallo-beta-lactamase binds a mercaptocarboxylate inhibitor and two zinc ions
  • Structure:2EY2: Klebsiella pneumonia NDM-1 metallo-beta-lactamase binds a methicillin and two zinc ions
  • Structure:Elizabethkingia meningoseptica BlaB metallo-beta-lactamase binds the inhibitor D-captopril and two zinc ions
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                     # #  #  ##                  #                          # ###    
1BC2_A     16 ISISQLNKNVWVHTELGSFn-GEAVPSNGLVLNTskGLVLVDSSWDDKLTKELIEMVEKKfqKRVTDVIITHAHADRIGG 94  Bacillus cereus
4WD6_A      7 LRIEKVKENIFLHTSYSRVngFGLVSSNGLVVIDkgNAFIVDTPWSDRDTETLVHWIRKNg-YELLGSVSTHWHEDRTAG 85  Pseudomonas stutzeri
3L6N_A      6 VIEPPIKNNLHIYKTFGVFg-GKEYSANSMYLVTkkGVVLFDVPWEKVQYQSLMDTIKKRhnLPVVAVFATHSHDDRAGD 84  Chryseobacterium i...
1M2X_A      2 VKIEKLKDNLYVYTTYNTFn-GTKYAANAVYLVTdkGVVVIDCPWGEDKFKSFTDEIYKKhgKKVIMNIATHSHDDRAGG 80  Elizabethkingia me...
2M5D_A     16 ISISQLNKNVWVHTELGSFn-GEAVPSNGLVLNTskGLVLVDSSWDDKLTKELIEMVEKKfqKRVTDVIITHAHADRIGG 94  Bacillus cereus
2YNT_A      7 LEVIKIEDGVYLHTSFKNIegYGLVDSNGLVVLDnnQAYIIDTPWSEEDTKLLLSWATDRg-YQVMASISTHSHEDRTAG 85  Pseudomonas aerugi...
EKF75539   32 LVVQPLTEHVWLHRSWTEYn-GQRVSSNGVIIDDgkTLTLIDTAWGEAPTATLLSWIEQHfgKDPDQAILTHSHDDRMGG 110 Alcanivorax hongde...
KGD61092   26 LQVYPLTEHTWVHRSWEVIq-GKRYPSNGLIIIEddTLTLIDTAWGEAPTARLLDWMEQQfgKAPDLAILTHSHDDRMGG 104 Alcanivorax jadens...
KGD63180   38 LQVYPLTDDVWVHRSWADLa-GTRYPANGLLINNddTLVLIDTAWGNDSTALLLEWVTVKfgKYPDVAILTHAHADRIGG 116 Alcanivorax sp. 19...
ABC80130   32 VWVRPISKDAWLIRSVSPIegWGDVESNAVLVAGaaESVLIDTPATEAQTAPVLAWAADTlrRPVRHLVVTHWHADRMGG 111 Anaeromyxobacter d...

Feature 1                                                                    #                
1BC2_A     95 IKTLKERGIKAHSTALTAELAKKNgYEEPLGDLQt--VTNLKfg-------nmKVETFYPGKGHTEDNIVVWLPQYNILV 165 Bacillus cereus
4WD6_A     86 IKWLNDQSISTYATTSTNHLLKENkKEPAKYTLKg-nESTLVd---------gLIEVFYPGGGHTIDNVVVWLPKSKILF 155 Pseudomonas stutzeri
3L6N_A     85 LSFFNNKGIKTYATAKTNEFLKKDgKATSTEIIKt--GKPYRig-------geEFVVDFLGEGHTADNVVVWFPKYNVLD 155 Chryseobacterium i...
1M2X_A     81 LEYFGKIGAKTYSTKMTDSILAKEnKPRAQYTFDn--NKSFKvg-------ksEFQVYYPGKGHTADNVVVWFPKEKVLV 151 Elizabethkingia me...
2M5D_A     95 IKTLKERGIKAHSTALTAELAKKNgYEEPLGDLQt--VTNLKfg-------nmKVETFYPGKGHTEDNIVVWLPQYNILV 165 Bacillus cereus
2YNT_A     86 IKLLNSKSIPTYTSELTKKLLAREgKPVPTHYFKd-dEFTLGn---------gLIELYYPGAGHTEDNIVAWLPKSKILF 155 Pseudomonas aerugi...
EKF75539  111 SRLLAEKHIRAYVHPKTLSIVKAEpGKYYDPASHf--PQALAfhdrr--arlgDIELYYPGAAHSPDNIVIWYGRDHLLV 186 Alcanivorax hongde...
KGD61092  105 ARTLAERGIPAWAHPATLTIARNDpDTFYDPDIPl--PRTLEafteqsrikfaNLELYYPGPAHSPDNIIVWYGGDEVLI 182 Alcanivorax jadens...
KGD63180  117 LPALAEHHIPAYVHPMTRELAASQyPEEQLPEVIp--GLEKHasa-----rleFLEAFYPGPAHSPDNIVVWYDSMKLLF 189 Alcanivorax sp. 19...
ABC80130  112 IGLARPRGIRTYALGKTRALARRHgLVVPERELRpeeRLVLAg---------vKVETWYPGHGHTADNLVVWLPADRLLV 182 Anaeromyxobacter d...

Feature 1       #  #      ###                             #                 
1BC2_A    166 GGCLVKstsaKDLGNVADAYVNEWSTSIENVLKRYRNINAVVPGHGEVGDKGLLLHTLDLLK 227 Bacillus cereus
4WD6_A    156 GGCFVRsldsEGLGYTGEAHIDQWSRSAQNALSRYSEAQIVIPGHGKIGDIALLKHTKSLAE 217 Pseudomonas stutzeri
3L6N_A    156 GGCLVKsnsaTDLGYIKEANVEQWPKTINKLKAKYSKATLIIPGHDEWKGGGHVEHTLELLN 217 Chryseobacterium indologenes
1M2X_A    152 GGCIIKsadsKDLGYIGEAYVNDWTQSVHNIQQKFSGAQYVVAGHDDWKDQRSIQHTLDLIN 213 Elizabethkingia meningoseptica
2M5D_A    166 GGCLVKstsaKDLGNVADAYVNEWSTSIENVLKRYRNINAVVPGHGEVGDKGLLLHTLDLLK 227 Bacillus cereus
2YNT_A    156 GGCLVRshewEGLGYVGDASISSWADSIKNIVSKKYPIQMVVPGHGKVGSSDILDHTIDLAE 217 Pseudomonas aeruginosa
EKF75539  187 GGCAVKgresTNIGYVAGSAPDHWAAAMDNLIQAFPQAAMVVPGHGQLGDEQLLFHTRALAT 248 Alcanivorax hongdengensis A-11-3
KGD61092  183 GGCAVKggsaTAIGYVEGSDANHWPVAMENVLTHYSEARIVVPGHGDIGDASLLTHTRDLAR 244 Alcanivorax jadensis T9
KGD63180  190 GGCAVKsadaGDIHYVEGSSPEGWEAAIGNIQGRYPEATTVIPGHGAVGGAQLLDHTRKLAR 251 Alcanivorax sp. 19-m-6
ABC80130  183 GGCFVKaaeaTTLGNLQEIDPVQWAKGVAATDRRYPEARTVVPGHGAAGGPELLRHTAALLR 244 Anaeromyxobacter dehalogenans 2CP-C

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