2FK6,1WW1,1ZKP,2CBN,4GCW


Conserved Protein Domain Family
RNaseZ_MBL-fold
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cd16272: RNaseZ_MBL-fold 
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Ribonuclease Z; MBL-fold metallo-hydrolase domain
The tRNA maturase RNase Z (also known as tRNase Z or 3' tRNase) catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Two forms of RNase Z exist in eukaryotes, one long (ELAC2) and one short form (ELAC1), the former may have resulted from a duplication of the shorter enzyme. Only the short form exists in bacteria. It includes the C-terminus of human ELAC2 and Escherichia coli zinc phosphodiesterase (ZiPD, also known as ecoZ, tRNase Z, or RNase BN) is a 3' tRNA-processing endonuclease, encoded by the elaC gene. Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions.
Links
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Statistics
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PSSM-Id: 293830
Aligned: 81 rows
Threshold Bit Score: 117.749
Created: 12-May-2015
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:putative active site [active site]
Evidence:
  • Comment:the N-terminal domain of eukaryotic RNase Z long form (ELAC2) exhibits poor conservation of metal binding site residues and may not have metal-dependent hydrolase activity
  • Comment:based on based on Bacillus subtilis RNaseZ and on other members of the MBL-fold metallo-hydrolase superfamily with structure and including two metal binding sites
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                        #                         ## # ##            
2FK6_A      3 LLFLGTGAGipak-arnVTSVALKLleerrsVWLFDCGEatQHQMLHTtik-prkIEKIFITHMAGDHVYGLPGLLGSRs 80  Bacillus subtilis
1WW1_A      2 NIIGFSKALf-------STWIYYSPe-----RILFDAGEgvSTTLGSKvy----aFKYVFLTHGHVDHIAGLWGVVNIRn 65  Thermotoga maritim...
4GCW_A      3 LLFLGTGAGipak-arnVTSVALKLleerrsVWLFDCGEatQHQMLHTtik-prkIEKIFITHMHGDHVYGLPGLLGSRs 80  Bacillus subtilis ...
Q8L633     76 VELESYAIEgis---vgGHETCVIVpe---lKCVFDIGR--CPSRAIQ-------QKFLFITHAHLDHIGGLPMYVASRg 140 thale cress
CAG33542   60 LQVVAAGSR--------DSGAALYVfse-fnRYLFNCGEg-VQRLMQEhklkvarLDNIFLTRMHWSNVGGLSGMILTLk 129 human
Q8LGU7      7 MQIEGYPIEgls---igGHETCIIFps---lRIAFDIGR--CPHRAIS-------QDFLFISHSHMDHIGGLPMYVATRg 71  thale cress
EAZ22222   29 LTVEGYPVEgis---igGQETCVIFpt---lSAAFDIGR--CPQRAVS-------QEFLFISHAHLDHIGGLPMYVATRg 93  Japanese rice
EDQ49751   22 LEVDGLSIEgvs---vgGQETCILIpa---lKLAFDIGR--CPERAIS-------QDFLFISHAHMDHIGGVCMYVATRg 86  Physcomitrella patens
AEA11548    3 LHIIGSGAGgspgskrwRAANLLETdg---gLVVVDCGVgcHYRLSDRgll--teIDYVFVTHSHMDHFLGLPEALFQAh 77  Thermoproteus uzon...
AET33185    3 LHILGSGAGgspgsrrwRSANLLDTge---gLVVIDCGVgcHYRLSDRgll--aeVDYVLVTHSHMDHYLGLPETLFQAh 77  Pyrobaculum sp. 1860

Feature 1                                                                                     
2FK6_A     81 fqg--gedELTVYGPkgikafietsl------------------------------------------------------ 104 Bacillus subtilis
1WW1_A     66 ngmgdrekPLDVFYPegnraveeytef----------------------------------------------------- 92  Thermotoga maritim...
4GCW_A     81 fqg--gedELTVYGPkgikafietsl------------------------------------------------------ 104 Bacillus subtilis ...
Q8L633    141 ly---nlePPKIFVPpsikedveklle----------------------------------------------------- 164 thale cress
CAG33542  130 et---glpKCVLSGPpqlekyleaikifsgplkgielavrphsapeyedetmtvyqipihseqrrgkhqpwqsperplsr 206 human
Q8LGU7     72 ly---kmkPPTIIVPasiketveslf------------------------------------------------------ 94  thale cress
EAZ22222   94 ly---rqrPPTIFIPaclrdpverlfe----------------------------------------------------- 117 Japanese rice
EDQ49751   87 lf---kmkPPTVIVPkclkptveklf------------------------------------------------------ 109 Physcomitrella patens
AEA11548   78 ieg--rrkRLVVVAPkivartieaas------------------------------------------------------ 101 Thermoproteus uzon...
AET33185   78 ieg--rrkPIHIFAPrvveeaarlva------------------------------------------------------ 101 Pyrobaculum sp. 1860

Feature 1                                                    ##                               
2FK6_A    105 --avtkthltypLAIQEIEe---GIVFEdd-----qfIVTAVSVI--HGVEAFGYRVQekdvpgslkadvlkemnippgp 172 Bacillus subtilis
1WW1_A     93 -ikranpdlrfsFNVHPLKe---GERVFlrnaggfkrYVQPFRTKhvSSEVSFGYHIFevrrklkkefqgldskeis--- 165 Thermotoga maritim...
4GCW_A    105 --avtkthltypLAIQEIEe---GIVFEdd-----qfIVTAVSVI--HGVEAFGYRVQekdvpgslkadvlkemnippgp 172 Bacillus subtilis ...
Q8L633    165 -ihrtmgqvelnVELIPLAv---GETYElrn----diVVRPFATH--HVIPSQGYVIYsvrkklqkqyahlkgkqi---- 230 thale cress
CAG33542  207 lsperssdselnENEPHLPh---GVSQRrgvr-dsslVVAFICKL--HLKRGNFLVLKakemglpv-------------- 266 human
Q8LGU7     95 --evhrkldsseLKHNLVGldigEEFIIrk-----dlKVKAFKTF--HVIQSQGYVVYstkyklkkeyiglsgnei---- 161 thale cress
EAZ22222  118 -lhrsmdqselsHNLVPLEi--gQEHELrr-----dlKVKAFKTY--HAIPSQITNTLtv-------------------- 167 Japanese rice
EDQ49751  110 --dvhreldgseLKHQLIGldigEEFNMgk-----nlVVKAFKTY--HVVPSQGYVVYsvknklkpeylglpgqki---- 176 Physcomitrella patens
AEA11548  102 --phllkninyqLEIRPAAp---GPLLEls-----glRVEAAQAC--HHTAEEAYAYRves------------------- 150 Thermoproteus uzon...
AET33185  102 --vnlfkapsyqIHFHRLAp---GTVVKqn-----tlVVEAGEAC--HHTAEEAYAFKisa------------------- 150 Pyrobaculum sp. 1860

Feature 1                                           #                             # 
2FK6_A    173 vyqkikkgetvtledgriingndfleppkkgrSVVFSGD----TRVSDKLKEla------rDCDVMVHEA 232 Bacillus subtilis
1WW1_A    166 --------------rlvkekgrdfvteeyhkkVLTISGD----SLALDPEEIr--------GTELLIHEC 209 Thermotoga maritima MSB8
4GCW_A    173 vyqkikkgetvtledgriingndfleppkkgrSVVFSGD----TRVSDKLKEla------rDCDVMVHEA 232 Bacillus subtilis subsp. sub...
Q8L633    231 ---------------ekikksgveitdtilspEIAFTGD----TTSEYMLDPrna---dalRAKVLITEA 278 thale cress
CAG33542  267 -------------------------gtaaiapIIAAVKDgksiTHEGREILAeelctppdpGAAFVVVEC 311 human
Q8LGU7    162 ---------------knlkvsgveitdsiitpEVAFTGD----TTSDFVVDEtna---dalKAKVLVMES 209 thale cress
EAZ22222  168 -------------------------------pEIAFTGD----TMADFILDPdna---dvlKAKILVVES 199 Japanese rice
EDQ49751  177 ---------------kelkmsgvqitdtlrvpEVAFTGD----TTPDFILDDani---dalQAKLLIMET 224 Physcomitrella patens
AEA11548  151 ------------------------------gaSLVFTGD----TSPNCEPVRrl-----srGVDVLVHEA 181 Thermoproteus uzoniensis 768-20
AET33185  151 -----------------------------rglDILFSGD----TGPGCEPVErl-----gqGVELAVLEA 182 Pyrobaculum sp. 1860

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