1UAZ,6JO0,3AM6,1E0P,3A7K,1XIO,4GYC,4HYJ,3UG9


Conserved Protein Domain Family
7tm_Opsins_type1

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cd14965: 7tm_Opsins_type1 
type 1 opsins, member of the seven-transmembrane GPCR superfamily
This group represents the microbial rhodopsin family, also known as type 1 rhodopsins, which can function as light-dependent ion pumps, cation channels, and sensors. They have been found in various single-celled microorganisms from all three domains of life, including halophile archaea, gamma-proteobacteria, cyanobacteria, fungi, and green algae. Members of the type I rhodopsin family include: light-driven inward chloride pump halorhodopsin (HR); light-driven outward proton pump bacteriorhodopsin (BR); light-gated cation channel channelrhodopsin (ChR); light-sensor activating transmembrane transducer proteins, sensory rhodopsin I and II (SRI and II); light-sensor activating soluble transducer protein Anabaena sensory rhodopsin (ASR); and other light-driven proton pumps such as blue-light-absorbing and green-light absorbing proteorhodopsins, among others. While microbial (type 1) and animal (type 2) rhodopsins have no sequence similarity with each other, they share a common architecture consisting of seven-transmembrane alpha-helices (TM) connected by extracellular loops and intracellular loops. Both types of rhodopsins consist of opsin and a covalently attached retinal (the aldehyde of vitamin A), a photoreactive chromophore, via a protonated Schiff base linkage to an amino group of lysine in the middle of the seventh transmembrane helix (TM7). Upon the absorption of light, microbial rhodopsins undergo light-induced photoisomerization of all-trans retinal into the 13-cis isomer, whereas the photoisomerization of 11-cis retinal to all-trans isomer occurs in the animal rhodopsins. While animal visual rhodopsins are activated by light to catalyze GDP/GTP exchange in the alpha subunit of the retinal G protein transducin (Gt), microbial rhodopsins do not activate G proteins.
Statistics
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PSSM-Id: 410629
Aligned: 21 rows
Threshold Bit Score: 117.775
Created: 10-May-2005
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1UAZ: Halorubrum chaoviator archaerhodopsin binds retinal, contacts at 4A.
    View structure with Cn3D
  • Structure:3AM6: Acetabularia acetabulum proton pumping rhodopsin Ar2 binds retinal, contacts at 4A.
    View structure with Cn3D
  • Structure:1E0P: Halobacterium salinarum bacteriorhodopsin binds retinal, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
1UAZ_A     15 ETLWLGIGTLLMLIGTFYFIVKGWGVTdk---eaREYYSITILVPGIASAAYLSMFFgigltevqv-------------- 77  Halobacterium sp.
6JO0_A     14 VKNTMIFSFLIQIITLIIGIFAQFIKVprhkyilKDALLLENIVQFIEAIFYLWFIYfykenv----------------- 76  Phaeocystis globos...
3A7K_A     34 LASSLYINIALAGLSILLFVFMTRGLDdp---raKLIAVSTILVPVVSIASYTGLASgltisvlempaghfaegssvmlg 110 Natronomonas phara...
4HYJ_A     11 TQYMFWVGFVGMAAGTLYFLVERNSLApe----yRSTATVAALVTFVAAIHYYFMKDavgtsgll--------------- 71  Exiguobacterium si...
P38079     32 GSDWLFTVFCVNLLFGVILVPLMFRKPvk----dRFVYYTAIAPNLFMSIAYFTMASnlgwipvrakynhvq----tstq 103 baker's yeast
Q9UW81     43 GHRTLWVTFALMVLSSGIFALLSWNVPts----kRLFHVITTLITVVASLSYFAMATghattfncdtawdhh---khvpd 115 Neurospora crassa
ACV05065   80 GQVFFWIEFGIQAVMFALFLILTLRREeh----kRRFHYITTLYSGISTMAYYCMARgqgsvalfdtws----------n 145 Cyanophora paradoxa
ACZ04975   45 GYDFFWLGFGFQVLATFVFLYLTLFHGka----aRKLHVLQLGVVGTAAVAYYAMARgqggvplpnd------------- 107 Cyanophora paradoxa
EKX50388   22 GEFFLAVSFLIMGVTTVAFVLMAFKAAhe----kRKFYFIMCYVTAISTFAYYAMLSgqgwlit---------------- 81  Guillardia theta C...
EKX51115   28 AGVFMVLAWFILTVTALFMAFKAVQADvs----iQKHYYLNAFICGISVFSYFAMFSgmgwetv---------------- 87  Guillardia theta C...
Feature 1                     #  ##                                            #   #          
1UAZ_A     78 --gsemldiyYARYADWLFTTPLLLLDLALLAKvd-----------------rVSIGTLVGVDALMIVTGLVGAlsht-- 136 Halobacterium sp.
6JO0_A     77 ----dkidiaKYRYYDWFLTTPTMILSVIIYFHynnsskkiyynmitffkqdfRKILELWFYNFNMLIIGYLQEini--- 149 Phaeocystis globos...
3A7K_A    111 geevdgvvtmWGRYLTWALSTPMILLALGLLAGsn-----------------aTKLFTAITFDIAMCVTGLAAAltts-- 171 Natronomonas phara...
4HYJ_A     72 --seidgfptEIRYIDWLVTTPLLLVKFPLLLGlkgr-------------lgrPLLTKLVIADVIMIVGGYIGEssinia 136 Exiguobacterium si...
P38079    104 kehpgyrqifYARYVGWFLAFPWPIIQMSLLGGtp-----------------lWQIAFNVGMTEIFTVCWLIAAcvh--- 163 baker's yeast
Q9UW81    116 tshqvcrqvfWGRYVDWALTTPLLLLELCLLAGvd-----------------gAHTLMAIVADVIMVLCGLFAAlgegg- 177 Neurospora crassa
ACV05065  146 gdfspyrvfyYARYIDWFLTVPLIILNLCLLASat-----------------kALTIGLALSGMLMVGTGLMGAlsi--- 205 Cyanophora paradoxa
ACZ04975  108 -vlsgfryfyWARYAEWAVTVPLTIYSLAVLADvr----------------iiETTLATIVASVIMVVCGLNGAlsq--- 167 Cyanophora paradoxa
EKX50388   82 ---pncrqlfYVRYVDWFFTTSLIFLNLGLIVGad-----------------tGLNIAIIAADMLMIFGGFMASiss--- 138 Guillardia theta C...
EKX51115   88 ---egcrqyfYIRHLEWALTCSLILFSLGILAEqd-----------------vATIFASMGFSVGMIYSGYLAAiglv-- 145 Guillardia theta C...
Feature 1               #  ##  #                                     #  ##  #                 
1UAZ_A    137 ---plaRYTWWLFSTICMIVVLYFLATSLRaaake-rgpevASTFNTLTALVLVLWTAYPILWIIGtegagvvglGIETL 212 Halobacterium sp.
6JO0_A    150 ----isILLSTLIGFYFFGLLFYKMFKYYVv---------qNKKNYLLFFLMFFIWGLYGIAALFNy--------KFKNA 208 Phaeocystis globos...
3A7K_A    172 --shlmRWFWYAISCACFIVVLYILLVEWAqdak---aagtADIFSTLKLLTVVMWLGYPIVWALGvegvavlpvGYTSW 246 Natronomonas phara...
4HYJ_A    137 ggftqlGLWSYLIGCFAWIYIIYLLFTNVTkaaen-kpapiRDALLKMRLFILIGWAIYPIGYAVTlfapgveiqLVREL 215 Exiguobacterium si...
P38079    164 ---styKWGYYTIGIGAAIVVCISLMTTTFnlvkargkdvsNVFITFMSVIMFLWLIAYPTCFGITdggn-vlqpDSATI 239 baker's yeast
Q9UW81    178 --ntaqKWGWYTIGCFSYLFVIWHVALHGSrtvta-kgrgvSRLFTGLAVFALLLWTAYPIIWGIAggar-rtnvDTEIL 253 Neurospora crassa
ACV05065  206 ---tgtKWGWFGISNMCFLPIVVAWVTVLRknare-kspevEKTYNILIIISIIAWFAYPIIWIIAtgth-aislAAEIV 280 Cyanophora paradoxa
ACZ04975  168 ---sssRWGWFGLGVWGLFLTLVGLFGNIRkaaya-ksesvGFTYTYLVFLILGSWIAYPVIWVIGegtr-avtvDVEIL 242 Cyanophora paradoxa
EKX50388  139 ---ghiKWLWFSLSLLIFAPLVYTMLRGYRalvdr-snpsvVEMYSKVAWIAAITWGLYPLVFIFSegtg-dwspNFEIM 213 Guillardia theta C...
EKX51115  146 ---plaKWLWFFFGLVLFVMVVYIILREFRqtlldkenpdkQQLFDKAALLTIVTWSLYPLVWILGpgig-avgvSVEAI 221 Guillardia theta C...
Feature 1          #  ##           
1UAZ_A    213 LFMVLDVTAKVGFGFILLRSR 233 Halobacterium sp.
6JO0_A    209 FYNILDIFSKNFFGLFLAYLV 229 Phaeocystis globosa virus 12T
3A7K_A    247 AYSALDIVAKYIFAFLLLNYL 267 Natronomonas pharaonis
4HYJ_A    216 IYNFADLTNKVGFGLIAFFAV 236 Exiguobacterium sibiricum
P38079    240 FYGIIDLLILSILPVLFMPLA 260 baker's yeast
Q9UW81    254 IYTVLDLLAKPVFGFWLLLSH 274 Neurospora crassa
ACV05065  281 SYAVVDTFSKALFGVILLFSR 301 Cyanophora paradoxa
ACZ04975  243 LYAIFDVITKVVFGFALLMSE 263 Cyanophora paradoxa
EKX50388  214 IYAVLDIMSKVVFGFVLLLSH 234 Guillardia theta CCMP2712
EKX51115  222 LYCFLDVTSKAVFSFVVVNVS 242 Guillardia theta CCMP2712

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