RNAse H3 (HIII) is a bacterial type 2 ribonuclease, which endonucleolytically hydrolyzes an RNA strand when it is annealed to a complementary DNA strand in the presence of divalent cations, and plays a role in DNA replication and repair. The N-terminal domain characterized by this model has been shown to be important in substrate binding; it might form initial contacts with the substrate and not be part of the active complex that involves the C-terminal ribonuclease domain. This domain has also been characterized as DUF3378.
Feature 1:nucleic acid substrate binding site [nucleic acid binding site]
Evidence:
Comment:Site directed mutagenesis of Bacillus stearothermophilus Ribonuclease H3. This domain may form initial contacts with the substrate, and not be part of the active complex that involves the C-terminal ribonuclease domain.
Jiyao W et al.(2023). "The conserved domain database in 2023.",