4KNC


Conserved Protein Domain Family
AlgX_N_like
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cd14439: AlgX_N_like 
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N-terminal catalytic domain of putative alginate O-acetyltranferase and similar proteins
The alginate biosynthesis protein AlgX appears to be directly involved in the O-acetylation of alginate, an exopolysaccharide that is associated with the formation of persistent biofilms, such as those by mucoid strains of Pseudomonas aeruginosa that affect patients suffering from cystic fibrosis. Its N-terminal catalytic domain resembles SGNH hydrolases, though with a permuted topology. The active site matches that of the SGNH hydrolases, is well conserved, and has been verified experimentally. This wider family includes AlgX, AlgJ, AlgV, and a number of uncharacterized families, some of which may have been mis-annotated in sequence databases.
Links
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Statistics
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PSSM-Id: 270205
Aligned: 15 rows
Threshold Bit Score: 223.484
Created: 2-Mar-2014
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active site
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 3 residue positions
Conserved feature residue pattern:D H SClick to see conserved feature residue pattern help
Evidence:
  • Comment:Catalytic triad characteristic for SGNH-hydrolases
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
4KNC_B        14 AGNLCPAAAYDSRyn-----------------------tKYLGFFTHLVQAqd-dWLFRtt-----------------yd 52  Pseudomonas aer...
WP_004886258  59 KRLGTNLWAALDYk------------------------lFNEGRPGVVLGKd--qWLYTd-------------------- 92  Pseudomonas vir...
WP_008074779  75 AQLPTTFAELERGasw---------------------llLGDTGPRVRSGCp--dWLFLad------------------e 113 Pseudomonas sp....
WP_009108129  71 LERHYNLRSLLITwnsfld---------------tfvlaSTSSSSQVMAGKg--hWLFLaqdgpr----------niled 123 Desulfovibrio s...
WP_017357517  22 CATLLATAPAVAQapaaa----------------papaaANQDSSIVLIGKd--gWLFPaw------------------g 65  Stenotrophomona...
WP_004082806  59 GDFMTKYESYLSDqfpgrniwrs-------ikvglsgigGSRKEGGVLIGKd--dYLMEe-------------------i 110 Clostridium sp....
WP_009958994  53 PDLAAVVKTLSPTqlaginalr---------arsaallrANLWPREDFIPAte-eFRLYkkc---------------lsc 107 Verrucomicrobiu...
WP_009962562  72 HHLREVEKVTDKAgysvalrqk---------aqsmlleyADEGNLKTYLGWe--gWLFYhpdlkaltgygpikpepfsvm 140 Verrucomicrobiu...
WP_009965497  23 TQADDFTSVVKGAl------------------------aASAATDLSVPGKgdsnWFFLrreleh---------lqngdl 69  Verrucomicrobiu...
WP_016217111  73 GNLIPTYAETSADflehlpfkrrmvdingvlakslnmrdLYRKNGGNVLKNg---YVVGi-------------------y 130 Dorea sp. 5-2

Feature 1                                                                                        
4KNC_B        53 LRTDFGts-----aEGWRELRALRDELKRkgIELVVVYQPTRGLVNr-----EKLSPAekag--fdyeLAKKNYLATIAR 120 Pseudomonas aer...
WP_004886258  93 EEFDAVangeqneaDNLALIQGVRDALEKqgTKLVLAIVPAKTRLYp-----EHIGDTkp------asLHADLYQQFHAQ 161 Pseudomonas vir...
WP_008074779 114 LKVNDQaqr--nadAKVQAVIDLKQQLSQrdISLLVVIVPDKSRIAs-----GQRCALhr------paVLEGRIQTWASK 180 Pseudomonas sp....
WP_009108129 124 ARSSDPlpee-svdVLVRELERRREWLEKrgIRYLVMVAPNKNTVYp-----ENLPEYlrp-----rkPEGHLQQLVNYA 192 Desulfovibrio s...
WP_017357517  66 SLTDVDapa---iaRNTALIADAKRALDAagVKLEVLVLPDKALFYe-----DRLPDGkrl-----gdAVRGRYALVHDG 132 Stenotrophomona...
WP_004082806 111 AVPDQEtl-----mENLEAIRQFENRSRD--VQMYMLLVPNAACILs-----ERLPAFa---------TVSDQSRMFAQV 169 Clostridium sp....
WP_009958994 108 LNAPTSry-----lEKTASLVEFHQLLRArgIRLILMPVPAAYAIHp-----DRVPGLdetgprpqpgDASLQLRAFGEA 177 Verrucomicrobiu...
WP_009962562 141 KDPELGrv-----pETRELIIEYAAQLKErgIDLLLVPVPLKPMIYn-----EQVAPQstp-----swVTHPDAPAFYSL 205 Verrucomicrobiu...
WP_009965497  70 AAADLSkank-egtDPVPVIAKYAKELKAlgVELLVVPVPAKASIYp-----EKLDAKve-------aSSVPALAPFLAK 136 Verrucomicrobiu...
WP_016217111 131 PYTSTD--------YEIQQITEFRSYLDK--KGIQLLYVNEPTKYIndqviiEDLGRVs---------YINDNTDRFLER 191 Dorea sp. 5-2

Feature 1                                                               # #                      
4KNC_B       121 FRQAGIWTPDFSPLf---------------------DEKe-------eHAYYFKgDHHWTPHGARRSAKIVAETLKQVPG 172 Pseudomonas aer...
WP_004886258 162 VAKAGIFAPDLLAPl--------------------qAAKq-------kGQVFLRtDTHWTPMGAEVAAQQLGVAIAQKTP 214 Pseudomonas vir...
WP_008074779 181 LDGAGVSNLDLTGA----------------------LTPl-------gASAWLRtDTHWSESGAKAAADAITQRLKTLGI 231 Pseudomonas sp....
WP_009108129 193 RAYTKLDIVDVTPGi--------------------lEQKk-------tNQVFYItDSHWNAHGAFAAYRQVIEALKKDFP 245 Desulfovibrio s...
WP_017357517 133 LEKAGVPAVDALQVl--------------------hQTKqg------gQDVFYRsDQHWTQAAADAVAVAVAAQVRQQVP 186 Stenotrophomona...
WP_004082806 170 KRELGEDVEWLDASa--------------------aLKKha------gEKIYYKtDHHWTSLGAFYTFSQVAEQMKIKTD 223 Clostridium sp....
WP_009958994 178 LRAAGIEFVDLSRPl--------------------gQAAte------gTLCYFHgDSHWTPLGAEVGAATVASLLAPKAG 231 Verrucomicrobiu...
WP_009962562 206 LRKNGVDVLDLTQDlsklrgerkrvfyleansqnreIAKeseealklkKDAFLKqDTHWTPEAMRLAAEKVADHIKAKYP 285 Verrucomicrobiu...
WP_009965497 137 LKAEGVEALDLEAEfkk-----------------vrTAEp-------aNQLYCAtDSHWSPAACRLVAHLVAAKYKTLPE 192 Verrucomicrobiu...
WP_016217111 192 LKDNGISYIDLRDDi---------------------VEKem-----dsFDLFYRtDHHWTAYAGKMAAESIADALNKNFG 245 Dorea sp. 5-2

Feature 1                                                                                        
4KNC_B       173 FEEIPKKQFeskr---vglLSKLGTFHKAAAQLCg-----------NSYATq---------YVDRFETEPVGAsdsg--- 226 Pseudomonas aer...
WP_004886258 215 LEGEPEQFVtqa----kgsESYKGDLTTFLPLDPlf---------sNMLPKpdel--qqrsTDPVQGEAAGDDal----- 274 Pseudomonas vir...
WP_008074779 232 SATPRRSFNesh----aplAVRPGDLVHLAGIDWlp---------lNWQPApemvaqtnthERPVTSADSPDSead---- 294 Pseudomonas sp....
WP_009108129 246 AIVPLERSQfsv----eqyNWLPGDLANMMGLAEhl---------kEDRIMy---------FNKDWYKARGLSydgptdp 303 Desulfovibrio s...
WP_017357517 187 TLAGEAGTGmalg--seikERRFGDLAELFLTDAq-----------RKAIGr---------DTFTVRRQSDQQsl----- 239 Stenotrophomona...
WP_004082806 224 VSSSFVSYPv--------sTTFNGMLAAKSGCRLn----------vKEEIYiyvp--rdtdNDVVVNYVDEQRkta---- 279 Clostridium sp....
WP_009958994 232 SFTPADKAFi---------REWMPPSRHAITPPPl-----------LQISVl---------SQNPVEARNPSTll----- 277 Verrucomicrobiu...
WP_009962562 286 QALGPGFRTirav--dgvhRSSLGDLVKLLDLKDp----------hQFFDE----------EEVFLNVIGEGTq------ 337 Verrucomicrobiu...
WP_009965497 193 VTAARLAGLttlp---aetREYHGDLLTDAQKGSva---------kEKLPI----------SRAGLGAGSSVTtv----- 245 Verrucomicrobiu...
WP_016217111 246 YNIDLSIYDddkfvykefkNAWLGEQGKKFGESFvglddfelilpeYDTLFhitkg-edviEGCFADVLVDQEcyl---- 320 Dorea sp. 5-2

Feature 1                                         #                                              
4KNC_B       227 -----dlfgDGGNPq------------IALVGTSNSgp------------------ayNFAGFLEEFSGADILNNAVSGg 271 Pseudomonas aer...
WP_004886258 275 -------faDSDIP-------------VGLVGTSYSanp-----------------nwNFVGALKEALRSDVVNYAEDGh 317 Pseudomonas vir...
WP_008074779 295 ------lfgDADLPn------------TALIGTSFSrn-------------------sNFAGFLQQALGAPVGNFAKDGg 337 Pseudomonas sp....
WP_009108129 304 hyfeypqysVTGNTkl---------pnAIVFHDSFWw---------------------ELLPFLAESFDKGLYVWLLPQt 353 Desulfovibrio s...
WP_017357517 240 -------ldDAPAP-------------VHVTGHSMVqp------------------yfGFPQKLSNVLDRPVSVNWKPGn 281 Stenotrophomona...
WP_004082806 280 ------slyDSSKLktr-------dqyAVFLGENTSvvdiktvses---qrrlllvkdSYANSFVPFLTPYFREIVLVDp 343 Clostridium sp....
WP_009958994 278 -------cdSNGIDef----------dAIEAPSSKArv------------------hgGFPSQVARAVGHRVDVITISGg 322 Verrucomicrobiu...
WP_009962562 338 ---------NSRSP-------------LVLLGDSFVniyddptlgfenpdkpdepirsGFAQHLSLLLQRPLDVIAKNGq 395 Verrucomicrobiu...
WP_009965497 246 -------esSPASP-------------IVVLGDSHLqvfrkgg--------nmldsqgGFVDHLQVALGTPVQEFTMQAg 297 Verrucomicrobiu...
WP_016217111 321 -------peNNEDVysasswhysymgqTGINGTKIEnrlnvegk-------rvlvlgdSYEQVTIPYLSLGISEIQCLVl 386 Dorea sp. 5-2

Feature 1                                                               
4KNC_B       272 -gFDSSLLAYMTSEEFHKnp----pKILIWEFAThy-------dmAQKSFYRQAM 314 Pseudomonas aeruginosa PAO1
WP_004886258 318 -gPILPMLKYLQTDAFKNtp----pQVVIWEFPErylp----ahnDLGEFDQQWI 363 Pseudomonas viridiflava
WP_008074779 338 -aFSGAANSYLNNPAFKEtp----pKQIIWEIPEr----------DLQTAYIDPI 377 Pseudomonas sp. GM79
WP_009108129 354 --NTEFRFFDTALIEREKp------DVVIDEFTEr----------YILPPLRGGF 390 Desulfovibrio sp. U5L
WP_017357517 282 vgHWVMLLEYLESPEFKQhk----pQVLVWQMFEps-------yvQGPDARGQWD 325 Stenotrophomonas maltophilia
WP_004082806 344 ryYSGTIEDIMDTYRITD-------VMFLYSGNTf----------FRDNNLKGVL 381 Clostridium sp. ASF502
WP_009958994 323 --GPAACRREFIRRCQMLpgygasrQTVLMVFAErc-------llLPLEAWPPLH 368 Verrucomicrobium spinosum
WP_009962562 396 --GSTEVRRELARRPDDEvr---ekKLVIWVIAArdvllstnaarAANITWARVK 445 Verrucomicrobium spinosum
WP_009965497 298 --GADGPRVEIARTSAKNpdfwskkKVAIWVFTAr---------eFTQGKWREIP 341 Verrucomicrobium spinosum
WP_016217111 387 rnYAGSVREYIENHEIDT-------VVIAYASFMi---------gAHDNETSANY 425 Dorea sp. 5-2

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