4G6V


Conserved Protein Domain Family
CDI_toxin_Bp1026b-like

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cd13442: CDI_toxin_Bp1026b-like 
Click on image for an interactive view with Cn3D
C-terminal (CT) toxin domain of the contact-dependent growth inhibition (CDI) system of Burkholderia pseudomallei 1026b, and related proteins
CDI toxins are expressed by gram-negative bacteria as part of a mechanism to inhibit the growth of neighboring cells. This model represents the C-terminal (CT) toxin domain of CdiA effector proteins. CdiA secretion is dependent on the outer membrane protein CdiB. Upon binding to a receptor on the surface of target bacteria, the CDI toxin is delivered via the C-terminal domain. A wide variety of C-terminal toxin domains appear to exist; this particular example from Burkholderia pseudomallei 1026b and other bacteria appears to function as a Mg2+-dependent RNAse cleaving tRNA, most likely in the aminoacyl acceptor stem. This CdiA-Ct is structurally similar to another CDI toxin domain from B. pseudomallei E479 which is unrelated in sequence but has a similar nuclease domain, and shares similar fold and active-site architecture; it contains a core alpha/beta-fold that is characteristic of PD(D/E)XK superfamily nucleases.
Statistics
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PSSM-Id: 412041
Aligned: 111 rows
Threshold Bit Score: 94.1475
Created: 30-Sep-2020
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
putative activeinhibitor
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1: putative active site [active site], 4 residue positions
Conserved feature residue pattern:[EQ] [DE] [DEQ] xClick to see conserved feature residue pattern help
Evidence:
  • Comment: nuclease active site
  • Comment:mutations of CdiA-CTII from Burkholderia pseudomallei 1026b Asp214 to Ala abolishes catalytic activity
  • Comment:putative metal binding site, based on CdiACTo11-EC869 (a Zn2+-dependent DNase with structure), encoded by the 11th "orphan" (o11) module of Escherichia coli O157:H7 strain EC869

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                               #                                       #        #        
4G6V_A         44 LSGKPTQIpplsdevTTRSLIRENQSAVTLANKGYDVVQNPEVl-------------gPKNPDYTINGQVFDNYAPATG- 109  Burkholderia ...
WP_145046727   88 PEGIYAEAtg----kDTRGIDRQNEAANVLAENGYRTIMLDEVpngdgfggngygidpRKSPDFIIEGQVFDCYAPEST- 162  Paenibacillus...
SDZ69274       50 PEGSKPRGnya--neVDRGIRRQNQTADLFAEKGYDIKMLDEVdggng-----fgknsTSNPDFLIEGNPFDCYSPEINt 122  Bacillus case...
WP_081848520  410 PKGNYEKNrp---gtDGQPIIRQNESADLLASEGYDIEMLPETkggng-----ygkkpTSNPDFLINGEAFDCYSPDGS- 480  Clostridium s...
KQO08496      538 PEGNYAKDtg----kDARGLKRQNEAADVLAEQGYRTTMLDEVpngnafdgngygidpSKSPDFIIEGQVFDCYAPDKGt 613  Paenibacillus...
SMF91582      570 PKGNYEKPdp----nDPRPITRQNETADLLAEKGYDIEMLPEVkggng-----ygvkpESNPDFLINEKVFDCYSPDTL- 639  Paenibacillus...
ERI09429      287 PKGSKPKGnyg--egDSHGIKKQNETADFLADKGYDIEMLDEIhggng-----ygilkGSNPDFLIEGNVFDCYAPKPDg 359  Aneurinibacil...
OOM03755       36 PKGGKPKGeya--tgKERGLAKQNETADLLAKDGYDISMLKEVqdekgn---gygikeGSNPDFLIENKPFDCYAPESTt 110  Clostridium r...
WP_133379600  788 PKGSKPKGnya--qgKDRGWIKQNESADLFAKEGYDIKMLDEInggng-----ygikeGSNPDFLIEGNVFDHYAPVSTk 860  Paenibacillus...
EKQ54365      907 PRGVYESAtgd--adTIRSITRQNEAADLLAKEGYDIEMLPYKvdgng-----eglipTANPDYKIMNNVFDCYSPSTS- 978  Clostridium s...
Feature 1                    #                                                              
4G6V_A        110 NVRNIATTISNKVSsgQASNIVVNLADSs------ASPAAIEAQINSYP-----IPGLGKVIVIDkLGNITIIK 172  Burkholderia pseudo...
WP_145046727  163 NLSTVLKMLRDKTTn-QARRIVLNLNDYpf----eKRTELIEFILSQTHk---dLKHLNELLVIE-GRQVTRAY 227  Paenibacillus xylan...
SDZ69274      123 SINNVSRTISKKTKn-QSERIVLNLDDYp-----aDKVDDLISVLLRKTnsngdLKYLQELFIVK-DGTITKVF 189  Bacillus caseinilyt...
WP_081848520  481 NIRNIWSTVEQKTIk-QADKIVLNLDDYt------GSIDELYKQFKSWD-----IENLKELLVIK-NGKITRWI 541  Clostridium sp. KNH...
KQO08496      614 SLQNILTTLRSKTTk-QARRIVLNLNDYp------VEKRRELIDYLISQth-kdLKHLDELLIIE-GRQVTRGY 678  Paenibacillus sp. L...
SMF91582      640 NVRNIWSTVVGKTEn-QASGIVLNLEDYt------GSMDKLVKQFDDWP-----IEGLDELFIIK-DGSITRWF 700  Paenibacillus uligi...
ERI09429      360 KVQSIIKELAGKTKk-QSGRIVLNLDDFpdnkvveVTETIFRKVNPNGD-----LKRLEELLIVK-DGTITRLF 426  Aneurinibacillus an...
OOM03755      111 GVNAICRNIAKKTKs-QAKRIVLNLDDYpsgevenVCNAILRKTNENVD-----LKRLQELFMVR-NKKITRLF 177  Clostridium roseum
WP_133379600  861 SANGIAEEIKRKTKs-QAERIVLNLDDFt-----dDQIAEVVRKILGKSnpngdLRRLQELFIVR-DGEIKLLY 927  Paenibacillus dendr...
EKQ54365      979 NVRNIWSTVQEKTLs-QGRRIVLNLNDYt------GSMDGLVKQFYDWP-----IESLDELIVIK-DGKIVTLF 1039 Clostridium sp. Mad...

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