CDD Conserved Protein Domain Family: RRM_CSTF2

cd12671: RRM_CSTF2_CSTF2T

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RNA recognition motif (RRM) found in cleavage stimulation factor subunit 2 (CSTF2), cleavage stimulation factor subunit 2 tau variant (CSTF2T) and similar proteins

This subgroup corresponds to the RRM domain of CSTF2, its tau variant and eukaryotic homologs. CSTF2, also termed cleavage stimulation factor 64 kDa subunit (CstF64), is the vertebrate conterpart of yeast mRNA 3'-end-processing protein RNA15. It is expressed in all somatic tissues and is one of three cleavage stimulatory factor (CstF) subunits required for polyadenylation. CstF64 contains an N-terminal RNA recognition motif (RRM), also known as RBD (RNA binding domain) or RNP (ribonucleoprotein domain), a CstF77-binding domain, a repeated MEARA helical region and a conserved C-terminal domain reported to bind the transcription factor PC-4. During polyadenylation, CstF interacts with the pre-mRNA through the RRM of CstF64 at U- or GU-rich sequences within 10 to 30 nucleotides downstream of the cleavage site. CSTF2T, also termed tauCstF64, is a paralog of the X-linked cleavage stimulation factor CstF64 protein that supports polyadenylation in most somatic cells. It is expressed during meiosis and subsequent haploid differentiation in a more limited set of tissues and cell types, largely in meiotic and postmeiotic male germ cells, and to a lesser extent in brain. The loss of CSTF2T will cause male infertility, as it is necessary for spermatogenesis and fertilization. Moreover, CSTF2T is required for expression of genes involved in morphological differentiation of spermatids, as well as for genes having products that function during interaction of motile spermatozoa with eggs. It promotes germ cell-specific patterns of polyadenylation by using its RRM to bind to different sequence elements downstream of polyadenylation sites than does CstF64.

Feature 1 # ##### ### # ## 1P1T_A 3 AVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRV 82 human NP_001006433 10 AVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRV 89 chicken XP_002590371 12 AQDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVISFRLVYDRETGKPKGYGFCEYKDQETALSAMRNLNGHELNGRQLRV 91 Florida lancelet EFX90110 8 VIDKSLRSVFVGNIPYDVTEEKLKDIFSEAGPVVSFKIVYDRETGKPKGYGFCEYRDQETALCAMRNLNGYEIAGRTLRV 87 common water flea XP_002112569 7 SRERSLRSVFVGNIPYEATEEQLKDIFGSAGPVVSFRLVYDRESGKPKGYGFCEFQDKETALSAMRNLSGYELNGRSLRV 86 Trichoplax adh... NP_477453 10 IMDKSMRSVFVGNIPYEATEEKLKEIFSEVGPVLSLKLVFDRESGKPKGFGFCEYKDQETALSAMRNLNGYEIGGRTLRV 89 fruit fly XP_006816057 9 ATDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYKDQETALSAMRNLSGYELNGRQLRV 88 Saccoglossus k... XP_014341592 12 AVDRSLRSVFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCEYQDQETALSAMRNLNGREFSGRALRV 91 coelacanth PIK33857 3 ASDKSQRSVFVGNIPYDTSEEKLKDIFSEVGPVLSFRLVYDRDTGKPKGYGFCEYQDEETALSAMRNLNGYDINGRALRV 82 Japanese sea c... PFX22936 22 ASDRSLRSVFVGNIPYEASEEQLKEIFSEVGPVVSFRLVFDRETGKPKGYGFCEYKDQETALSAIRNLNGYELNGRALRV 101 Stylophora pis...

Feature 1 # 1P1T_A 83 DNAAS 87 human NP_001006433 90 DNAAS 94 chicken XP_002590371 92 DNAAS 96 Florida lancelet EFX90110 88 DNACT 92 common water flea XP_002112569 87 DSAAS 91 Trichoplax adhaerens NP_477453 90 DNACT 94 fruit fly XP_006816057 89 DNAAS 93 Saccoglossus kowalevskii XP_014341592 92 DNAAS 96 coelacanth PIK33857 83 DYATT 87 Japanese sea cucumber PFX22936 102 DSAAS 106 Stylophora pistillata

1P1T

Conserved Protein Domain FamilyRRM_CSTF2_CSTF2T

Conserved Protein Domain Family
RRM_CSTF2_CSTF2T