2QFJ,2KXF,3UWT


Conserved Protein Domain Family
RRM2_PUF60

?
cd12371: RRM2_PUF60 
Click on image for an interactive view with Cn3D
RNA recognition motif 2 (RRM2) found in (U)-binding-splicing factor PUF60 and similar proteins
This subfamily corresponds to the RRM2 of PUF60, also termed FUSE-binding protein-interacting repressor (FBP-interacting repressor or FIR), or Ro-binding protein 1 (RoBP1), or Siah-binding protein 1 (Siah-BP1). PUF60 is an essential splicing factor that functions as a poly-U RNA-binding protein required to reconstitute splicing in depleted nuclear extracts. Its function is enhanced through interaction with U2 auxiliary factor U2AF65. PUF60 also controls human c-myc gene expression by binding and inhibiting the transcription factor far upstream sequence element (FUSE)-binding-protein (FBP), an activator of c-myc promoters. PUF60 contains two central RNA recognition motifs (RRMs), also termed RBDs (RNA binding domains) or RNPs (ribonucleoprotein domains), and a C-terminal U2AF (U2 auxiliary factor) homology motifs (UHM) that harbors another RRM and binds to tryptophan-containing linear peptide motifs (UHM ligand motifs, ULMs) in several nuclear proteins. Research indicates that PUF60 binds FUSE as a dimer, and only the first two RRM domains participate in the single-stranded DNA recognition.
Statistics
?
PSSM-Id: 409806
Aligned: 22 rows
Threshold Bit Score: 135.876
Created: 13-Sep-2011
Updated: 25-Oct-2021
Structure
?
Program:
Drawing:
Aligned Rows:
 
dimer interface
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:2QFJ; Homo sapiens FIR (FBP-interacting repressor) protein, with two tandem RRM domains, binds another FIR protein upon binding DNA, contacts at 4A.
    View structure with Cn3D

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                 #   ##                
2QFJ_A        126 NRIYVASVHQDLSDDDIKSVFEAFGKIKSATLArdpt-tgkHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKA 202  human
XP_001638369  161 ARIYIASVHPDLLEDDIKSVFEAFGKVVHCSLSkepm-tgkHKGYGFIEYENQQSANDAIASMNLFDLGGQFLRVGRA 237  starlet sea ane...
EFX90167      217 NRIFIASVHPDLTEEDIKSVFEAFGKIKMCQLAqgpa-pgkHRGYGFIEYETTQSAHDAIASMNMFDLGGQHLRVGRA 293  common water flea
XP_002114842  263 ARIYVASVHPDLSESDIKSVFEAFGKIKSCTMPldni-tgkHRGYCYIELDGLQSAMDAIASMNMFDLGGQYLRVGRA 339  Trichoplax adha...
XP_002408027  172 NRIYIASVHQDLTEADIQSVFEAFGKIRTCKLVpsst-pgkHKEYGFIEYETNVGANEAIASMNLFDLGGQYLRVGRA 248  black-legged tick
NP_491177     207 FRVYVSSVHPDLSETDLKSVFEAFGEIVKCQLArapt-grgHRGFGYLEFNNLTSQSEAIAGMNMFDLGGQYLRVGKC 283  nematode
CCD58930      190 PRVYIASVHPELTESDIQTVFEAFGKVNSCSLYpdpkcsgrHRGFGYIDFESEEAAIAAVSSMNCFDLAGQQLRVGRA 267  Schistosoma man...
EDQ91638      116 HRIYVSSIHPDLGELEIKSVFESFGPVTRVILApgaegkgnHRGYGWVDFEEQKSVPDAIKSMNLFDLGGQFLRVSIG 193  Monosiga brevic...
CBY18091      224 NRIYVASVHENLSDDDIRAVFEAFGRVVSCSLVrdvsepetHCGYGYIEFENPESMDEAVKAMDQYDLGGKMLRVCAC 301  Oikopleura dioica
EFA83511      873 NRIYVGSIPWNVNEDQIKVIFSSIGNVVSCSLMpnle-sgrHKGFGFIDYDNSKSAEDAIATLNGYDIGGRQLKVGRP 949  Polysphondylium...

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap