2QRK,2QRJ,2QRL,3UHA


Conserved Protein Domain Family
SDH
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cd12188: SDH 
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Saccharopine Dehydrogenase NAD-binding and catalytic domains
Saccharopine Dehydrogenase (SDH) catalyzes the final step in the reversible NAD-dependent oxidative deamination of saccharopine to alpha-ketoglutarate and lysine, in the alpha-aminoadipate pathway of L-lysine biosynthesis. SHD is structurally related to formate dehydrogenase and similar enzymes, having a 2-domain structure in which a Rossmann-fold NAD(P)-binding domain is inserted within the linear sequence of a catalytic domain of related structure.
Links
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Statistics
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PSSM-Id: 240664
Aligned: 29 rows
Threshold Bit Score: 420.87
Created: 11-Mar-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 22 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD(P) binding site [chemical binding site]
Evidence:
  • Structure:3UHA: Saccharomyces Cervisiae Saccharopine Dehydrogenase bound to NAD; contacts at 4A
  • Structure:2QRK: Saccharomyces cerevisiae Saccharopine Dehydrogenase bound to AMP; contacts at 4A
  • Comment:Saccharomyces Cervisiae Saccharopine Dehydrogenase retains the 3D arrangement of the GxGxxG(17-18x)D NAD binding site motif, but has altered spacing between the conserved residues: GxxGxxG(17-20x)D
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
2QRK_A     25 VTLHLRAETKPLEARAALTPTTVKKLIAKGFKIYVEDSPQSTFNINEYRQAGAIIVpaGSWK-TAPRDRIIIGLKEMPEt 103 baker's yeast
3UHA_A      4 VTLHLRAETKPLEARAALTPTTVKKLIAKGFKIYVEDSPQSTFNINEYRQAGAIIVpaGSWK-TAPRDRIIIGLKEMPEt 82  Saccharomyces cere...
EAR52854    1 MHLWLRAEARATERRTPLLPEGAARLIAGGVQVSVERSATRVIADAAYAAVGCTLAppGSWA-SAPVEAVILGLKELPE- 78  Oceanicola granulo...
ABD56932    1 MHLWIRAEERANERRVGITPVGVSRLLANGLDITVEDSPIRVIPIDGFRQSGAQIAaaGSWR-SAPEDAVIFGLKELPE- 78  Jannaschia sp. CCS1
ABF62840    2 THLWVRAEQRLNEDRVGLTPAGAKALLEAGIRVTVEESSSRAIPLQGYIDAGCEIApeNAWP-DAPRDAIIFGLKELPD- 79  Silicibacter sp. T...
BAJ03898    4 AHIWLRAESKPLEERIALTPNVAQKLLQAGFKITVEESPLSAIPAQAYQEIGCDVMpaQSWQ-QAPKDTIILGLKELSE- 81  Shewanella violace...
EAU52959    2 THLWIRAEERPNEKRVGVTPNGVKSLLQAGFDVTIERDTTRAISIESYSGAQVAGT--GDWR-SAPKEAIIIGLKELPE- 77  Rhodobacterales ba...
EDY61149    4 LHLWLRHEVRSTERRTPLVPSDARRLVDSGVTLTVEESPQRIFPIEQYAAVGCRVApaGSWAsRAPEDAVVVGLKELPD- 82  Streptomyces svice...
CBW25926    3 RLIWLRHETKAFEQRCCLTPSACKELLELGHDVVVESSATRVYEDSEYSEVGCKIVetNSWV-NAPLNAIIVGLKELED- 80  Bacteriovorax mari...
EFL18468    5 PQLWMRSETRPTERRAPLTPEDALRLVRQGVRITVEESGHRVFPLDDYEAAGCRTApaGSWSrAAPGSAYVLGLKELPP- 83  Streptomyces sp. C

Feature 1                                                    #     #  #                       
2QRK_A    104 DTFPLVHEHIQFAHCYKDQAGWQNVLMRFIKGHGTLYDLEFLENDqGRRVAAFGFYAGFAGAALGVRDWAFKQthsd--- 180 baker's yeast
3UHA_A     83 DTFPLVHEHIQFAHCYKDQAGWQNVLMRFIKGHGTLYDLEFLENDqGRRVAAFGFYAGFAGAALGVRDWAFKQthsd--- 159 Saccharomyces cere...
EAR52854   79 SDAPLIHRHIFFAHAYKGQPGADALLDRFRRGGGTLLDLEYLVDAdGRRVAAFGYWAGYVGAAVSLLAYAAQDgq----- 153 Oceanicola granulo...
ABD56932   79 DTGPLRHRHILFGHAFKGQADGAHLLRRFRDGGGALYDLEYLVDEtGRRVAAFGYWAGFVGAALSVAAWAAQHagr---- 154 Jannaschia sp. CCS1
ABF62840   80 DGTPLPHRHIMFGHAFKGQHSGKELLRRFREGGGTLYDLEYLVDPsGRRVAAFGYWAGYAGAAVTLKAWAAQQrge---- 155 Silicibacter sp. T...
BAJ03898   82 NNWPLVHRHIHFAHVYKEQQGWQDVLRRFKTGEGELYDLEYLVDDnNRRVAAFGYWAGFAGAAVALKAFGKRQqvannas 161 Shewanella violace...
EAU52959   78 EITPLHHRHIMFGHAFKDQPDGQKLLERFQTGSGTLYDLEYLTDDtGRRVAAFGYWAGYAGAAVAIKSWAAAQkgn---- 153 Rhodobacterales ba...
EDY61149   83 EPAALTHRHVFFGHAYKEQPGAEELLRRFAAGGGALLDLEYLVDDhGRRLAAFGYWAGYLGAALAVLQHRGRLa------ 156 Streptomyces svice...
CBW25926   81 ANFPLSHRHIHFAHVFKNQHGFEKTLSRFVAGDGLLFDLEYLVNKdEKRIAAFGVWAGFAGAALGLDLWVHTQlgmn--- 157 Bacteriovorax mari...
EFL18468   84 APELLHHRHVYFGHAYKGQAQAPVLLRRFAAGGGTLLDLEYLADAqGRRLAAFGYWAGYAGAALAALHARGALr------ 157 Streptomyces sp. C

Feature 1                                                ## ####                      ##  #   
2QRK_A    181 ----dedlPAVSPYPNEKALVKDVTKdykealatGARKPTVLIIGALGRCGSGAIDLLH-KVGIpdaNILKWDIKETSrG 255 baker's yeast
3UHA_A    160 ----dedlPAVSPYPNEKALVKDVTKdykealatGARKPTVLIIGALGRSGSGAIDLLH-KVGIpdaNILKWDIKETSrG 234 Saccharomyces cere...
EAR52854  154 -------lGPVAPWADAAAMRAEVTAa------lGEARPATLVIGARGRVGTGAADLVTrDLGL---PVTRWDMEETAgG 217 Oceanicola granulo...
ABD56932  155 ------tlDPVAPWPDQTALIDDVQTrlt---aaQAPLPTVLIIGALGRTGTGASAFLN-ALGI---QPTAWDMAETAhG 221 Jannaschia sp. CCS1
ABF62840  156 ------tcGPVGVYKNKDALLADLGReld---atGADRPKAIVIGALGRVGTGAADLCE-AMGV---AVTKWDMNETAsG 222 Silicibacter sp. T...
BAJ03898  162 tqdsqsvlAPLTSMPSKQALVDDVAKsl----stLARPPKVLVIGAKGRSGRGAVEMAE-SVGA---EVTQWDMAETKiG 233 Shewanella violace...
EAU52959  154 ------icQPLQTFASAKDLNADVASd------lGKKNPNVIIIGANGRVGSGAQDFCA-ALGA---SVTCWDIEETAhG 217 Rhodobacterales ba...
EDY61149  157 --------APLTPTSQEELQEALRPVa-------GDEEFTALVIGALGRSGRGARVALR-AAGV---EPTCWDLDETR-D 216 Streptomyces svice...
CBW25926  158 ----ynsrAPLLPYENQMHLINDVQDrl----nkIISRPRVLIIGAKGRSGKGAVKFLR-ALGI---EPTQWGSADTAsE 225 Bacteriovorax mari...
EFL18468  158 --------PPLVPLPRPELDARLRAA--------APDGLRALVIGAGGRSGRGACDALA-TAGI---PVTRWDRTSTR-S 216 Streptomyces sp. C

Feature 1                    ##    #                        ##                                
2QRK_A    256 GPFDEIPQADIFINCIYLSKPIAPFTNMEKLNNPn---RRLRTVVDVSADTTNPHNPIPIYTVaTVFNKPTVLVPttagp 332 baker's yeast
3UHA_A    235 GPFDEIPQADIFINCIYLSKPIAPFTNMEKLNNPn---RRLRTVVDVSADTTNPHNPIPIYTVaTVFNKPTVLVPttagp 311 Saccharomyces cere...
EAR52854  218 GPFPQIAEHELLVNAVLALPGVPVFAGSELLHAP----RRLRVIGDVACDPGTPYNPIPLYDAaTGWDAPALRVHdt--p 291 Oceanicola granulo...
ABD56932  222 GSFPQVLSHTMFLNCILAMDGVPVFVPATAKDAP----RALRVIGDIACDPSSDFSPIKVYDRvTTWAEPTLRAAda--p 295 Jannaschia sp. CCS1
ABF62840  223 GPFPEILDHDLFLNCIFARPGTPVFVPKEALTAA----RNLTAIGDVACDPDSDYNPVPVYSRaTTWDAPVVRVAnd--p 296 Silicibacter sp. T...
BAJ03898  234 GPFEAILDFDVLVNCVFVQEALPPFITLPMLETNgiepRRLTIICDVSCDPYGTYNPLPIYSScTTFDKPCLRIIeg-dn 312 Shewanella violace...
EAU52959  218 GPFPEILDHDIFLNCILANKNTPVFVPKKAKIGP----RKLMVIGDIACDPGSFYSPIKVYDCaTSWEKPALRVHkd--k 291 Rhodobacterales ba...
EDY61149  217 LDRPALLAHDVLVNCVLATSPIPPFVREADLDDPa---RRLRTLSDVTCDVGSPLNVLPVYDRtTEWAEPVRRLHke--p 291 Streptomyces svice...
CBW25926  226 HPIEDILGYDILINCALIKEPRAPFLDLETIKKQ----RNLTVISDVGCDPNGPCNPLPIYKDcTTMDMPSIKILd---- 297 Bacteriovorax mari...
EFL18468  217 LDRAALLGHDILVNAVLTTRPVPPFLTPADLEAGg--pRRLSVIADITCDTGSELHLLPVYDRlTDWTAPARRLPgg-gp 293 Streptomyces sp. C

Feature 1           #####                                            
2QRK_A    333 KLSVISIDHLPSLLPREASEFFSHDLLPSLELLPQrk-taPVWVRAKKLFDRHCA 386 baker's yeast
3UHA_A    312 KLSVISIDHLPSLLPREASEFFSHDLLPSLELLPQrk-taPVWVRAKKLFDRHCA 365 Saccharomyces cerevisiae S288c
EAR52854  292 PLDIMAIDNLPSLLPAEASTEFAADLLEHLLQLPRg---gGVWARAEATFRKHLG 343 Oceanicola granulosus HTCC2516
ABD56932  296 PLDVMAIDNLPSLLPLESSTDFAEQLLPHLLALPQid--eGVWARAHASYQHALG 348 Jannaschia sp. CCS1
ABF62840  297 VLDVMAIDNLPSMLPAESSEDYADQLLASLLTLSDld--aGVWGRAKAVFDEHVA 349 Silicibacter sp. TM1040
BAJ03898  313 PVDLIAIDHLPSLLPVESSEDFCQQLLPHLLQLDDln--lGVWQKANQIFTDKLS 365 Shewanella violacea DSS12
EAU52959  292 ILDITAIDNLPSLLPRESSEDFAAQLLPHLMMLQEie--kGVWGKACAIFNEYIS 344 Rhodobacterales bacterium HTCC2255
EDY61149  292 PLDLIAIDNLPSLLPEESSVGFSGSLLPLLLEFGVg----GPWARCLDRFHQACR 342 Streptomyces sviceus ATCC 29083
CBW25926  298 DLHLTAIDHLPSLLPRESSEDFCSQLLPHFKEFLEgniedTPWDKALEVFYTKTH 352 Bacteriovorax marinus SJ
EFL18468  294 PVDVIAIDNLPSLLPAEAARAFSADLLPKLAALHGps-pdPDWLRCRAAFTTALA 347 Streptomyces sp. C

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