1WP9


Conserved Protein Domain Family
Hef_ID
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cd12089: Hef_ID 
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insert domain of Archaeal Hef helicase/nuclease
Archaeal Hef helicase/nuclease, originally identified in the hyperthermophilic archaeon Pyrococcus furiosus, contains an N-terminal SF2 helicase domain and a C-terminal XPF/Mus81-type nuclease domain. Hef has been shown to process flap- or fork-DNA structures, and that both helicase and nuclease domain independently recognize branched DNA, with a strong preference for the forked DNA. The SF2 helicase domain is comprised of 3 structural domains, the 2 generally conserved helicase domains and a helical domain inserted between the two domains. This domain which is not present in all SF2 helicases, has been shown to play an important role in branched structure processing.
Links
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Statistics
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PSSM-Id: 277188
Aligned: 50 rows
Threshold Bit Score: 87.6506
Created: 22-May-2012
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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helicase domain
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:helicase domain interface [polypeptide binding site]
Evidence:
  • Comment:the helical_insert_domain contacts the most C-terminal of the two helicase structural domains of the SF2 domain
  • Structure:1WP9, Pyrococcus furiosus Hef helical_insert_domain/helicase interface, contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1           #                                                                           #
1WP9_A       216 KEVRKLLREMLRDALKPLAETGlless--spdiPKKEVLRAGQIINEemak---gnhdLRGLLLYHAMALKLHHAIELLE 290  Pyrococcus fur...
ADK25987     228 RYIASVLRSMLDERLKWLVQRGllgkkkdtrwiFKRDLIEAGEALRYrleltmeeqrgPIYVAIMNQSAALTLYCLELIE 307  Candidatus Nit...
BAJ47980     224 EEVRKAIREAIENRVRHLQRFGvvrvp--vehvNRTILVQAMARIRAeidsg--vkgqNMHYMALISEILSLYHAQELIE 299  uncultured cre...
YP_003483474 219 RELYGKLKELYNSIITELRKFGlfst---lkkpTRRDVLRAQKIVQQeikd---gkseFYQAAMLITMAIKIDYALEYLE 292  Aciduliprofund...
NP_112009    211 GAIKDLLKSALDSVLKPLKDQGikv------grTRKDMAESIQNLINrake----drsLFPLVRHLTAAIRIDYIIEYIE 280  Thermoplasma v...
NP_394951    209 KEIVNMIDAVMSKLLEPVKSSNfsi------grSRKDLVATMQKVINsake----drsLFQLVRRITAAIRLDYLKEYIE 278  Thermoplasma a...
BAE95223     261 RRIREHVERALQSRLEELKRLGviss----trvNMSNLIEARESISS-----------IKGSTVPLYSAIRLSHALKVLD 325  unclutured Can...
AAC62691     217 KEIQKLLKMALDERYAALKRCGydlg----snrSLSALLRLRMVVLSg----------NRRAAKPLFTAIRITYALNIFE 282  Cenarchaeum sy...
YP_001582278 218 KTIQTLLKLALDQRYQTLRDNGikla----eqqSLSALLRIRQFVLNq----------NRRSAKPLFTAIRIHYALNILE 283  Nitrosopumilus...
ACF09868     227 KLMAAPLQMWLEHLVDQLRRLGyhvh---sgraTSSQLNETRGRIQGaivr---geglAYNAARQCAQAQRLLNLIGYLL 300  uncultured mar...

Feature 1        ##  #                                         
1WP9_A       291 TQGLSALRAYIKKLYEEaka-gstKASKEIFSDKRMKKAISLLVQA 335  Pyrococcus furiosus DSM 3638
ADK25987     308 SQGAHSLRAFFERIEEEg-----gRAHAALLKDPKIMEVRALVEKA 348  Candidatus Nitrososphaera gargensis
BAJ47980     300 SQHIHSLREYLREVMHSe-----lRSHRMLSGSEDFRKIIQLAAKS 340  uncultured crenarchaeote 10-H-08
YP_003483474 293 TQGFEAAYNYLLRIIEEgnskggsKAARTLVRDERFIEMMKIARKI 338  Aciduliprofundum boonei T469
NP_112009    281 TQGLDVAYQYVKDMEESqdq-sikRAYSMMKSLDDFRKAIENMKNV 325  Thermoplasma volcanium GSS1
NP_394951    279 TQGIDVALHYLEEMEKSqdp-girRAISMLSSLDEFATLRTKLMLA 323  Thermoplasma acidophilum DSM 1728
BAE95223     326 TQGITAFTRFYERLREKkg----gVGVRSLVEDRELKSAYELARGA 367  unclutured Candidatus Nitrosocaldus sp.
AAC62691     283 AHGVTPFLKFCERTVKKkg----aGVAELFEEDRNFTGAMARAKAA 324  Cenarchaeum symbiosum
YP_001582278 284 AHGITPFLKFCERAKAKkg----aGVKELFEVDPNFTRAVHLAKEA 325  Nitrosopumilus maritimus SCM1
ACF09868     301 SQGVAASREYLGRVKNSgen--gdKGASAFFNDSRITELFENLKVS 344  uncultured marine group II euryarchaeote KM3-136...

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