Conserved Protein Domain Family
SH3_DBS
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cd11857: SH3_DBS 
Src homology 3 domain of DBL's Big Sister (DBS), a guanine nucleotide exchange factor
DBS, also called MCF2L (MCF2-transforming sequence-like protein) or OST, is a Rho GTPase guanine nucleotide exchange factor (RhoGEF), facilitating the exchange of GDP and GTP. It was originally isolated from a cDNA screen for sequences that cause malignant growth. It plays roles in regulating clathrin-mediated endocytosis and cell migration through its activation of Rac1 and Cdc42. Depending on cell type, DBS can also activate RhoA and RhoG. DBS contains a Sec14-like domain, spectrin-like repeats, a RhoGEF [or Dbl homology (DH)] domain, a Pleckstrin homology (PH) domain, and an SH3 domain. SH3 domains are protein interaction domains that bind to proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs. They play versatile and diverse roles in the cell including the regulation of enzymes, changing the subcellular localization of signaling pathway components, and mediating the formation of multiprotein complex assemblies.
Links
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Statistics
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PSSM-Id: 212791
Aligned: 15 rows
Threshold Bit Score: 83.4898
Created: 2-Jun-2011
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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peptide ligand
Feature 1:peptide ligand binding site [polypeptide binding site]
Evidence:
  • Comment:based on the binding of peptide ligands to the SH3 domains of other superfamily members
  • Comment:SH3 domains typically bind proline-rich ligands, preferentially to PxxP motifs.
  • Citation:PMID 7664083
  • Citation:PMID 7735837
  • Comment:flanking hinge and loops (RT and n-Src) confer sequence specificity for ligand residues outside the core binding motif
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1              # #  #   #                 ##              # ##   
O15068       1059 KYTVVADHEKGgpDALRVRSGDVVELVQEGDEGLWYVRDPTTGKEGWVPASSLSV 1113 human
XP_003218795  998 KYTAMANYDKGgsEDLVLKSGDLVQLIHEGEDGLWYIRNLSTSREGWMPINCLLA 1052 green anole
XP_001515228 1300 KYSIVADPENGgtDVLAVKSGDLVELISEGTERQWYVRNLTTSREGWIPTGSLSA 1354 platypus
XP_002920409 1045 KYKALADCRKRgpEDLLIRNGDVIQFLHEDAEGQWLVKNLNRRKEGLIPGNAAQI 1099 giant panda
XP_003341916  293 LFRVEGSYETCspDSLTLEDGDLVQFVQEGENGQWLVKKLVSEKSDWVPSSLLHP 347  gray short-tailed opossum
XP_685307    1014 RYKVFMDWRNNglDNLMIKCGDVIQLQHEEDHGQWLVKNLSRRQEGRVPTPNLQC 1068 zebrafish
XP_003445209  992 RYRVLADCLQNgpDSIIIKRGDVIQLQCEDNRGRWLVKNVTLQQEGFLPAATLQL 1046 Nile tilapia
CAG09815     1190 EEEVLVQLNKP--DSVAIHPGDVIQLKSEDAEGRWLVKNLSVRQEGFIPAARLPP 1242 spotted green pufferfish
XP_002937885 1132 LFRVEGCLEVCspSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIP 1186 western clawed frog
NP_001238992 1160 KYTVVSDYEKGvsEEFAVKGGDLVQLIREGEEGLWYVKNLTTGREGWIPANNLLM 1214 chicken

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