Nucleoside Triphosphate Pyrophosphohydrolase (EC 3.6.1.8) MazG-like domain found in Exiguobacterium sibiricum YP_001813558.1 protein and its bacterial homologs
This family contains a putative NTP_PPase (YP_001813558.1) from Exiguobacterium sibiricum and its bacterial homologs. Unlike normal dimeric dUTPase-like proteins with a central four-helix bundle forming the active site, YP_001813558.1 displays a very unusual interlaced segment-swapped dimer that might be important for it to adapt to an extremely cold environment. Moreover, structural analysis and comparisons indicate that YP_001813558.1 potentially prefers to hydrolyze dCTPs or its derivatives.
Feature 1: putative metal binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:E E A D
Evidence:
Comment:The metal-binding motif of the superfamily (EXXEX(12-28)EXXD) is well-conserved in this family, except for the third Glu residue replaced by Asp.
Comment:Based on structure evidence of Deinococcus radiodurans DR2231 protein binding Mg2+.
Comment:Most members of the NTP-PPase superfamily contain the well-conserved divalent ion-binding motif, EEED, instead of EEAD present here.
Jiyao W et al.(2023). "The conserved domain database in 2023.",