3CRC,1VMG,1W2Y,2A7W,2GTA,2P06,2Q73,2YEU,3CRC,3OBC


Conserved Protein Domain Family
NTP-PPase
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cd11523: NTP-PPase 
Click on image for an interactive view with Cn3D
Nucleoside Triphosphate Pyrophosphohydrolase (EC 3.6.1.8) MazG-like domain superfamily
This superfamily contains enzymes that hydrolyze the alpha-beta phosphodiester bond of all canonical NTPs into monophosphate derivatives and pyrophosphate (PPi). Divalent ions, such as Mg2+ ion(s), are essential to activate a proposed water nucleophile and stabilize the charged intermediates to facilitate catalysis. These enzymes share a conserved divalent ion-binding motif EXX[E/D] in their active sites. They also share a highly conserved four-helix bundle, where one face forms the active site, while the other participates in oligomer assembly. The four-helix bundle consists of two central antiparallel alpha-helices that can be contained within a single protomer or form upon dimerization. The superfamily members include dimeric dUTP pyrophosphatases (dUTPases; EC 3.6.1.23), the nonspecific NTP-PPase MazG proteins, HisE-encoded phosphoribosyl ATP pyrophosphohydolase (PRA-PH), fungal histidine biosynthesis trifunctional proteins, and several uncharacterized protein families.
Links
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Statistics
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PSSM-Id: 212133
Aligned: 47 rows
Threshold Bit Score: 36.5969
Created: 5-Apr-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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metal binding
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1: metal binding site [ion binding site], 4 residue positions
Conserved feature residue pattern:E E E DClick to see conserved feature residue pattern help
Evidence:
  • Comment:Most of members in all-alpha-NTP pyrophosphohydrolase superfamily contain a well-conserved divalent ion-binding motif EEED.
  • Structure:2Q73; Vibrio dat 722 iMazG protein binds Mg2+ ion; contacts at 4.0A
  • Structure:3OBC; Archaeoglobus fulgidus pyrophosphatase (Af1178) binds Zn2+ ion; contacts at 4.0A
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                           #  #                           #  #          
3CRC_B       148 QKIQKRCAnvgfd---------wttlgPVVDKVYEEIDEVMYEArqavv----------dqakLEEEMGDLLFATVNLAR 208 Escherichia col...
1W2Y_A        11 LKLQQKLNdetnglnwengytkegkliSWRRCIYMECAELIDSFtwkhwkni---ssltnwenVRIEIVDIWHFILSLLL 87  Campylobacter j...
2A7W_A        10 ADTLEARReaapqssyv-aslfhkgedAILKKVAEEAAETLXASkdkd------------klhLVREVADLWFHTXVLLT 76  Chromobacterium...
2P06_A        35 XHAKYXKRvsrpgntpr--pwfdfseeRLLSRLFEEXDELREAVeked------------wenLRDELLDVANFCXYLWG 100 Archaeoglobus f...
2Q73_A         7 QSHIKEFDyape------------qseHYFFKLIEEVGELSESIrkgksgqp---tldelkgsVAEELYDVLYYVCALAN 71  Vibrio sp. DAT722
3OBC_A        17 LDILREFRdsrgwl-------kyhtpkNLAVSISIEVAELLEIFqwtrssdeefevlerrkgeVEEEIADVLIYLLFLCD 89  Archaeoglobus f...
NP_456010     10 TALYRLALqrf-------------gpdAQALKLTEEAAELAASAarnlng-------qgsesdLAAELADVEIMTEQLRL 69  Salmonella ente...
NP_633958     13 RDRIPDILrdsgrdfavkeisdpefllELERKLEEELAEYLESK-------------------EAEELADLLEVIYRIAE 73  Methanosarcina ...
Q87I30        12 TRKSQYDQtntwf----------kgveTYLEAIGKEVDEVREEIredr------------lchLEDELGDVLWNYLNVLK 69  Vibrio parahaem...
YP_001447772 187 RRKAKYDNtntwy----------kgseTYFDALYQELEEVGEEItqtr------------lcyLEDELGDVLWNYLNLLM 244 Vibrio harveyi ...

Feature 1          
3CRC_B       209 HL 210 Escherichia coli K-12
1W2Y_A        88 EE 89  Campylobacter jejuni
2A7W_A        77 YH 78  Chromobacterium violaceum
2P06_A       101 KL 102 Archaeoglobus fulgidus
2Q73_A        72 IH 73  Vibrio sp. DAT722
3OBC_A        90 VA 91  Archaeoglobus fulgidus
NP_456010     70 QG 71  Salmonella enterica subsp. enterica serovar Typhi str. CT18
NP_633958     74 LR 75  Methanosarcina mazei Go1
Q87I30        70 TL 71  Vibrio parahaemolyticus
YP_001447772 245 AL 246 Vibrio harveyi ATCC BAA-1116

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