2RCH,3DAM,2RCL


Conserved Protein Domain Family
CYP74

?
cd11071: CYP74 
Click on image for an interactive view with Cn3D
cytochrome P450 family 74
The cytochrome P450 74 (CYP74) family controls several enzymatic conversions of fatty acid hydroperoxides to bioactive oxylipins in plants, some invertebrates, and bacteria. It includes two dehydrases, namely allene oxide synthase (AOS) and divinyl ether synthase (DES), and two isomerases, hydroperoxide lyase (HPL) and epoxyalcohol synthase (EAS). AOS (EC 4.2.1.92, also called hydroperoxide dehydratase), such as Arabidopsis thaliana CYP74A acts on a number of unsaturated fatty-acid hydroperoxides, forming the corresponding allene oxides. DES (EC 4.2.1.121), also called colneleate synthase or CYP74D, catalyzes the selective removal of pro-R hydrogen at C-8 in the biosynthesis of colneleic acid. The linolenate HPL, Arabidopsis thaliana CYP74B2, is required for the synthesis of the green leaf volatiles (GLVs) hexanal and trans-2-hexenal. The fatty acid HPL, Solanum lycopersicum CYP74B, is involved in the biosynthesis of traumatin and C6 aldehydes. The epoxyalcohol synthase Ranunculus japonicus CYP74A88 (also known as RjEAS) specifically converts linoleic acid 9- and 13-hydroperoxides to oxiranyl carbinols 9,10-epoxy-11-hydroxy-12-octadecenoic acid and 11-hydroxy-12,13-epoxy-9-octadecenoic acid, respectively. The CYP74 family belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
?
PSSM-Id: 410694
Aligned: 64 rows
Threshold Bit Score: 294.938
Created: 5-Feb-2010
Updated: 25-Oct-2021
Structure
?
Program:
Drawing:
Aligned Rows:
 
heme bindingchemical
Conserved site includes 26 residues -Click on image for an interactive view with Cn3D
Feature 1:heme binding site [chemical binding site]
Evidence:

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                    #   #            
2RCH_A     59 KSRIRKYN------------STVYRVNmppgaf----iaenpQVVALLdGKSFPVLFDVDKVEKKDLFTGtympste--- 119  thale cress
EDX74399   10 HERQLKFSrllakigqnnpiGKVIRLRay------------fLDLPVL-TKQWRDYLYVRKERISDKFLGldqgiihtsy 76   Microcoleus chtho...
KST62073   10 HERQLWFA------------RAIAKVGsssplgqlltlsayfTDLPLL-IQNWRDYLYQRKEHISDKFLGldraiiytsy 76   Mastigocoleus tes...
EDV28127   46 KTLQQKYQ------------QNVFRINig------------vRAIALVdNRVIRILFQNDKVTKEDGVGNyitny----- 96   Trichoplax adhaerens
ACD42778   18 YKRRRKYK------------SSVFKVNmg------------vKGIHVCdKKAMKVFFDMSKIYKEPAFGRlhynic---- 69   elkhorn coral
PFX21200   19 QKRMKDLQ------------SSVFKCSff-----------gaPFVAITdHEGIQCLFDQKLVEKEPGFGNfrfnqs---- 71   Stylophora pistil...
RDD38707   41 KQYEEKYN------------STVFRVYag------------aKVVALLdNIAIQTLFDHDSISKEDGFGKvrlnfd---- 92   Trichoplax sp. H2
ACD88492   16 QTLKPNREe-----------DFVEKNIg-------------fPCRVVTgNKAVQSVFDIELFKKEEFCFGvaevrr---- 67  
EDV28129   38 KTIQEKTG------------HTIFRINlg------------vEAFALCdSQSATAVFDYSKVTKDDGFGRlkfnkn---- 89   Trichoplax adhaerens
RMX60482   35 YKRRDKYK------------CSVFKVNmg------------iRGIHICdRKGINILFNMEKIYKEPAFGRlnynic---- 86   Pocillopora damic...
Feature 1                                     ##        #   #  #   #                          
2RCH_A    120 -------------------------ltggyriLSYLDPSEPKHEKLKNLLFFLLkssr---nriFPEFQATYSELFDSLE 171  thale cress
EDX74399   77 adakrlvettpqqrdnylgplkinarsyflghPLCLGTNGDEHAGIRALFFHALpep----eeiTEVLAKLVDEELAKAT 152  Microcoleus chtho...
KST62073   77 agvkklvetvpqkrgnylgplridarsyflgnPLSLGTNGEKHDGIRALILQALp--------dPSQTTEILAHLVDLAL 148  Mastigocoleus tes...
EDV28127   97 ----------------------------nlmdSLRPSIFSDQKDRMSRNSFILQtlaamqdkyiIQQTYNSIEDHFQRWN 148  Trichoplax adhaerens
ACD42778   70 ---------------------------lldgyTPSMFSNGIPHQKQKAFLVEICkiaq--rskiFDTSLKLIKEYSRNWE 120  elkhorn coral
PFX21200   72 ---------------------------llgdhVPSMFSNGDRHRQQKAFLIAVSqnmlr-mgrhIPNIMDIMPEHLIKWN 123  Stylophora pistil...
RDD38707   93 ---------------------------lmaniRPSIFSNDQSHIRRKRFIMQILrhip--anymIKIADKIIRRHFEAWS 143  Trichoplax sp. H2
ACD88492   68 --------------------------dftegiCPSVSSNGKIHEKNKGFLMEVIakag--egipSSTASSVLSNISKWGS 119 
EDV28129   90 ---------------------------ltgqiRPSIFCNGEVHERYRKFLMDVFrats--vdylIKVTNEAVEKHFSLWE 140  Trichoplax adhaerens
RMX60482   87 ---------------------------lldgyTPSMFANGIPHEKQKAFLMQLCkiaq--qgkiFETSVKLLREYSTLWQ 137  Pocillopora damic...
Feature 1                                                                                     
2RCH_A    172 kelslkg----------------------kadfggSSDGTAFNFLARAFYGtnpadt---------------klkadAPG 214  thale cress
EDX74399  153 kpgii--------------------------dvktDLPKMIVALLHELVLNlklse-----------------eeiaGSV 189  Microcoleus chtho...
KST62073  149 aravgwg----------------------klhvkdDIPKLMVALLHELIFEiklse-----------------eeinASV 189  Mastigocoleus tes...
EDV28127  149 ktyqptask------------------siqpswddGIQHLCCDIICRLFIDkt-----------------------vNFE 187  Trichoplax adhaerens
ACD42778  121 kads---------------------------qlraTWELSIMDLISDIFTEa------------------------fLGT 149  elkhorn coral
PFX21200  124 fkegenkcisdipgakevksekrgmgnpsqeeweeRILHLAMDVLSESMLGirlsghsflqwrfevlkeertcfgkpSGK 203  Stylophora pistil...
RDD38707  144 nkysyshre-----------------nsvagdfesEIQEVCSDIITTLFLGsk-----------------------gNVA 183  Trichoplax sp. H2
ACD88492  120 tams---------------------------dfesKLLAVAADTVLPNIFGess---------------------sfNAE 151 
EDV28129  141 ksytgag---------------------iakewemDILRLCSDIVHRAILGge-----------------------iSSE 176  Trichoplax adhaerens
RMX60482  138 nadptl-----------------------kstweqSIMDLTCDIFTEAFLGirvdhqamf---------kcvkgswgKRG 185  Pocillopora damic...
Feature 1                                                                                     
2RCH_A    215 LITKWVLFNLHPLLSIGlprvieepli----htfslppalvKSDYQRLYEFFLESAGeilvead--klgisrEEATHNLL 288  thale cress
EDX74399  190 GYIGGLGLATVPNLIHNtllrnktrshiq-hrqglvdtykkSPKYQDYLGFGATHQLn-------------eQQVANNLF 255  Microcoleus chtho...
KST62073  190 AYIDNLGLATVPNPIHTtvlnsqtrshiq-hrknliatykkSPRYQEYLTIGANYQLs-------------eQEVLNNIF 255  Mastigocoleus tes...
EDV28127  188 SIFQWYVQALQKIKVPGllkqr--------------qcadiYNTYIELFKEIENSTYlpyylntgrqcslsdEYAKENVL 253  Trichoplax adhaerens
ACD42778  150 RLDQKYMYNFLKGSWGKgrvlkkameaag-clkqtlqgtkgSSDVIEILKLAVNAGIt-------------eDQALMDIL 215  elkhorn coral
PFX21200  204 YKKALHAFNHLKDAIAN------------------------APDIDPVLKEAANHGLt-------------eEEAVLEIL 246  Stylophora pistil...
RDD38707  184 ALQNWISGIFERSRRLGlmktk--------------syvnaYIGYQEMMKHIERAPTlkkivqiagehsldvEDAKHNLL 249  Trichoplax sp. H2
ACD88492  152 DIRLYIIGATEVRSCIVkaltsknldeerqamrsiigkiktSERYQQLMDLGHAYGLg-------------eAETTAQLL 218 
EDV28129  177 ASHNWLMGILKKKRSALgvktkffskaiv-gyailtngikeSPHYQSMIKLADKRSVs-------------eEEALNNIL 242  Trichoplax adhaerens
RMX60482  186 TLRKGIDGASCLKQAFRq-----------------------SPALADIIKIAVEAGVt-------------eEQALMDVL 229  Pocillopora damic...
Feature 1          ##  ##  #                                                         ##      #
2RCH_A    289 FATCFNTWGGMKILFPNMVKRIGRAghqVHNRLAEEIRSVIksnggeltmgaiekMELTKSVVYECLRFEPPV----TAQ 364  thale cress
EDX74399  256 DMIHVAGTAGTSDLLASVFGVLCQDe-aLRANVSAEIDTVWdgqtppn-aqtlaqLELTEKVIFETARLYPPV----RFL 329  Microcoleus chtho...
KST62073  256 DTIHIAGTAGTSALLGSVFGVLCLDe-qLKANVISELDLVWdgnetpn-trsvdeLKITEKIILETARLYPPV----RFV 329  Mastigocoleus tes...
EDV28127  254 FGIIFNAFGSCEAIMRTCAARIGILsheDRNQLRQEIQSTLgegndls-laaltkMKRLRSFVAEVLRTSSPI----SLI 328  Trichoplax adhaerens
ACD42778  216 FMLNFNAYGGVSGVLRTCLARLYVLeedYKQRMKNELKTILsnkels--easleeMILLHNFILEVLRMHPPV----PVF 289  elkhorn coral
PFX21200  247 WMLNFNAAPGTGAAMRSAAARLSIMnceERNELKNEIRECLgpigln--lktlhkMKKLDNLVCEVLRMYPPV----ALY 320  Stylophora pistil...
RDD38707  250 FAMVFNAYGGCNCVIRTCAARIALLkeaERERLIAEIKLRLenhdgls-anslyqMRTLESFVLEVLRTSPPI----SMI 324  Trichoplax sp. H2
ACD88492  219 LPVFFNGVAGIRANLVSSFARLDTIsaeDREELREEALAALkkhgglt-resleeMPKMESFVLEVLRACPSPmfwsTIA 297 
EDV28129  243 FSILFNYYGGCSAAFRTCAARISILdenIKSELIEDIKSAIdkygglt-gqalcqMRKLHSFVLENLRMSSPV----NLI 317  Trichoplax adhaerens
RMX60482  230 FMLNFNAYGGVSGSLRTCMARLFVLeqdYKQKMKNEMITVLsnqals--kealteMPLLHNFILEVLRMHPPV----PVF 303  Pocillopora damic...
Feature 1                                                                          # #        
2RCH_A    365 YGRAKKDLVIeshdaaFKVKAGEMLYGYQPLATRDpki-----fdrADEFVPERFvgeeg-ekllrHVLWSNGPetetpt 438  thale cress
EDX74399  330 NQMSTEPMEVeiggqrCPFQKGTRLVASLFTANRDpdk-----yeePDTFKTTRDyr--------dLLSWNGSN------ 390  Microcoleus chtho...
KST62073  330 SQFSQSPQEIefagkkCPFQTGTRLVASIFTANRDsqk-----yknPDDFDIERDys--------dILSWNGEN------ 390  Mastigocoleus tes...
EDV28127  329 FRRAKQDMFIqaytgtYKISKGHLLVGNIHLAHRDpnv-----fpePDTFKPFRFyddp---elirHMIWEAGVypdeak 400  Trichoplax adhaerens
ACD42778  290 FGRARDDFSLetecgtFIVRKDQLLVGNVHMAHRDssi-----fdqPDKFMPSRFedes----vidHIIYGYGPfhqeat 360  elkhorn coral
PFX21200  321 FGIARRAFFVktssgiYKIDKGQKVVGNCHLAQRDpkvfgisgcegVDEFDPSRFnvkg----lksKLICTHGPlsdpps 396  Stylophora pistil...
RDD38707  325 FGRTKRDLVIysssgkFKVVKGQLLAGSIYSAHRDpti-----fknPEEFRPFRFieep---alkqNIVWEAGPytvnat 396  Trichoplax sp. H2
ACD88492  298 TRPTTVEYTTdsgehaLKIKEGERVYASSYWALRDpav-----fdkPEDFMWRRFlgpegdarrkhHVIFHGRLtdt-pa 371 
EDV28129  318 FGRAVEDLMIksntgtYKIPKGKLMVANLFWAHRDtrv-----fddPLTFQALRFfnnp---dlknYLYWEAGPfskdqc 389  Trichoplax adhaerens
RMX60482  304 FGRAREDFILnsdsgkFIVKKDELLVGNVRMVHRDeri-----fdqPHKFLPARFenka----lidHIIYGYGPfneeat 374  Pocillopora damic...
Feature 1       ######  ##                                   
2RCH_A    439 vGNKQCAGKDFVVLVARLFVIEIFRRYD-SFDIEVGTspLGSSVNFS 484  thale cress
EDX74399  391 -QERNCPGRALSIAIVKLFCLYAFKHYQwRSVSGVEWdmKKFAPFKP 436  Microcoleus chthonoplastes PCC 7420
KST62073  391 -IERKCPGQSLSIALIKLFCLYAFKKYQwNCSSEVEWdmKRFGAFTP 436  Mastigocoleus testarum BC008
EDV28127  401 nLSHQCPGKKMMMILLQLFCMKLLIHTEfQFDVTPDWneKHLHSLGC 447  Trichoplax adhaerens
ACD42778  361 pQNQRCPGQDITLQILKVCLSFILQNCEyALADAPKWtgKRLRRIGC 407  elkhorn coral
PFX21200  397 pENHKCTGTNVGYVTLKCFVLYLIMFCDwSLEERPFWtdKSLIRYGN 443  Stylophora pistillata
RDD38707  397 eDSHQCPGKGIAIPLIEVFCLYLLLHCQyTLNKEPIWtgKKSSRSGC 443  Trichoplax sp. H2
ACD88492  372 vNNHMCPGRDVSLSALKGSIAIFNTFFGwELQEPPVWtgTKMTRGSQ 418 
EDV28129  390 eKTHQCPGRNIAIPSIMLFSAYLLLQGDyQLGSEPQWtgKKIRRLGT 436  Trichoplax adhaerens
RMX60482  375 pQNHHCAGQEITLTVLKVAVAHVVLNCEfGLVDQPVWtgKKLRRVGC 421  Pocillopora damicornis

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap