1IO8,2RFB,1UE8,4GGV,4E2P,3ZKP,1IO8,4RM4,4YT3,1F4T


Conserved Protein Domain Family
P450_EryK-like

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cd11032: P450_EryK-like 
Click on image for an interactive view with Cn3D
cytochrome P450 EryK and similar cytochrome P450s
This subfamily contains archaeal and bacterial CYPs including Saccharopolyspora erythraea P450 EryK, Saccharolobus solfataricus cytochrome P450 119 (CYP119), Picrophilus torridus CYP231A2, Bacillus subtilis CYP109, Streptomyces himastatinicus HmtT and HmtN, and Bacillus megaterium CYP106A2, among others. EryK, also called erythromycin C-12 hydroxylase, is active during the final steps of erythromycin A (ErA) biosynthesis. CYP106A2 catalyzes the hydroxylation of a variety of 3-oxo-delta(4)-steroids such as progesterone and deoxycorticosterone, mainly in the 15beta-position. It is also capable of hydroxylating a variety of terpenoids. HmtT and HmtN is involved in the post-tailoring of the cyclohexadepsipeptide backbone during the biosynthesis of the himastatin antibiotic. The EryK-like subfamily belongs to the large cytochrome P450 (P450, CYP) superfamily of heme-containing proteins that catalyze a variety of oxidative reactions of a large number of structurally different endogenous and exogenous compounds in organisms from all major domains of life. CYPs bind their diverse ligands in a buried, hydrophobic active site, which is accessed through a substrate access channel formed by two flexible helices and their connecting loop.
Statistics
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PSSM-Id: 410658
Aligned: 39 rows
Threshold Bit Score: 429.712
Created: 28-Jan-2010
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
 
heme bindingchemical
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:heme binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                  ##      #   #      
1IO8_B      9 RKKDPVYYDG--NIWQVLSYRYTKEVLNNFSKFSSdltgyherledlrngkirfdiptryTMLTSDPPLHDELRSMSADI 86  Sulfolobus solfata...
2RFB_A      1 MRLNDPVHYD--GAWHVYKYSDVKHVLMNDKIFSSnpgnry-------------snaggiSFITMDNPEHKEFRDISAPY 65  Picrophilus torridus
1UE8_A      9 RKESPVYYDG--KVWNLFKYEDCKMVLNDHKRFSSnltgyndklemlrsgkvffdiptryTMLTSDPPLHDELRNLTADA 86  Sulfolobus tokodaii
1IO8_A      9 RKKDPVYYDG--NIWQVLSYRYTKEVLNNFSKFSSdltgyherledlrngkirfdiptryTMLTSDPPLHDELRSMSADI 86  Sulfolobus solfata...
4YT3_A     30 LENDPVSYHEgtDTWNVFKYEDVKRVLSDYKHFSSvrkrttisvgt---dseegsvpekiQITESDPPDHRKRRSLLAAA 106 Bacillus megaterium
1F4T_A      9 RKKDPVYYDG--NIWQVFSYRYTKEVLNNFSKFSSdltgyherledlrngkirfdiptryTMLTSDPPLHDELRSMSADI 86  Sulfolobus solfata...
P14762     31 LNNSPVYFHEetNTWNVFQYEHVKQVLSNYDFFSSdgqrttifvgd---nskkkstspitNLTNLDPPDHRKARSLLAAA 107 Bacillus megateriu...
ACX68487   21 RTESPVTAIDggGAWNVFKYEDVKAVFTNYEVFSSqgssss-------------ddpiesSVLRQDPPKHRQLRKLVSHA 87  Geobacillus sp. Y4...
EEM10559   33 RENHPVFYDAeqDVWNVFLYEHAKRVLFDHQLFSNkkersfipi--------pkimdnrsNVNFCDPPQHRNRRALLAKA 104 Bacillus pseudomyc...
P27632     40 RRESPVRYDPlrDCWDVFKYDDVQFVLKNPKLFSSkrgi------------------qteSILTMDPPKHTKLRALVSRA 101 Bacillus subtilis ...
Feature 1     #                                           #                                   
1IO8_B     87 FSPQKLqtLETFIRETTRSLLDSIDPre-dDIVKkLAVPLPIIVISKILGLPiedKEKFKEWSDLVAFrlgkpg------ 159 Sulfolobus solfata...
2RFB_A     66 FLPSKIndYKDFIEETSNDLIKNIDNk---DIISeYAVRLPVNIISKILGIPdsdMPLFKLWSDYIIGnkrd-------- 134 Picrophilus torridus
1UE8_A     87 FNPSNL--PVDFVREVTVKLLSELDEe--fDVIEsFAIPLPILVISKMLGINp-dVKKVKDWSDLVALrlgrad------ 155 Sulfolobus tokodaii
1IO8_A     87 FSPQKLqtLETFIRETTRSLLDSIDPre-dDIVKkLAVPLPIIVISKILGLPiedKEKFKEWSDLVAFrlgkpg------ 159 Sulfolobus solfata...
4YT3_A    107 FTPRSLqnWEPRIQEIADELIGQMDGgteiDIVAsLASPLPIIVMADLMGVPskdRLLFKKWVDTLFLpfdrekq---ee 183 Bacillus megaterium
1F4T_A     87 FSPQKLqtLETFIRETTRSLLDSIDPre-dDIVKkLAVPLPIIVISKILGLPiedKEKFKEWSDLVAFrlgkpg------ 159 Sulfolobus solfata...
P14762    108 FTPRSLknWEPRIKQIAADLVEAIQKnstiNIVDdLSSPFPSLVIADLFGVPvkdRYQFKKWVDILFQpydqerl---ee 184 Bacillus megateriu...
ACX68487   88 FTPRMIesLAPKIQEITTSLLDEAEKkgkmDIVAdLASPLPITVIAEMLGVSmeyRERFKAWSDALVGdna--------- 158 Geobacillus sp. Y4...
EEM10559  105 FTPRSLesWEPRIQAIVDELLIDMEGqsavDIIQkLAIPLPVIVIAELLGVPskdRAMIKSWSDILFFpyekety---sd 181 Bacillus pseudomyc...
P27632    102 FTPKAVkqLETRIKDVTAFLLQEARQkstiDIIEdFAGPLPVIIIAEMLGAPiedRHLIKTYSDVLVAgakdssdkavad 181 Bacillus subtilis ...
Feature 1                                                            ##  ##  ##  #            
1IO8_B    160 eifelGKKYLELIGYVKDHLNSg-----TEVVSRVVNSN-----LSDIEKLGYIILLLIAGNETTTNLISNSVIDFTRFN 229 Sulfolobus solfata...
2RFB_A    135 --enfNYVNNRMVSRLLEIFKSd----sHGIINVLAGSSlknrkLTMDEKIKYIMLLIIGGNETTTNLIGNMIRVIDENP 208 Picrophilus torridus
1UE8_A    156 eifsiGRKYLELISFSKKELDSrkgkeiVDLTGKIANSN-----LSELEKEGYFILLMIAGNETTTNLIGNAIEDFTLYN 230 Sulfolobus tokodaii
1IO8_A    160 eifelGKKYLELIGYVKDHLNSg-----TEVVSRVVNSN-----LSDIEKLGYIILLLIAGNETTTNLISNSVIDFTRFN 229 Sulfolobus solfata...
4YT3_A    184 vdklkQVAAKEYYQYLYPIVVQkrlnpaDDIISDLLKSEvdgemFTDDEVVRTTMLILGAGVETTSHLLANSFYSLLYDD 263 Bacillus megaterium
1F4T_A    160 eifelGKKYLELIGYVKDHLNSg-----TEVVSRVVNSN-----LSDIEKLGYIILLLIAGNETTTNLISNSVIDFTRFN 229 Sulfolobus solfata...
P14762    185 ieqekQRAGAEYFQYLYPIVIEkrsnlsDDIISDLIQAEvdgetFTDEEIVHATMLLLGAGVETTSHAIANMFYSFLYDD 264 Bacillus megateriu...
ACX68487  159 --dayYQCQREMSEYFSEIAEDrrrhpqDDLITKLVEARidnehLTELEIIGFCILLLVAGNETTTNLISSAVLAFDSLP 236 Geobacillus sp. Y4...
EEM10559  182 ivtkkEEAMKEFYYYLYPIVLEkrkhpaDDIISDLTKAElegerLTEEEVVISAIGLLGAGNETTTTLISNIFYSMLFDK 261 Bacillus pseudomyc...
P27632    182 mvhnrRDGHAFLSDYFRDILSKrraepkEDLMTMLLQAEidgeyLTEEQLIGFCILLLVAGNETTTNLIANAVRYLTEDS 261 Bacillus subtilis ...
Feature 1                               #    # #                      #                       
1IO8_B    230 --LWQRIREEn-lYLKAIEEALRYSPPv-MRTVRKTKERVKLGDQTIEEGEYVRVWIASANRDEEVFHDGEKFIPDRNpn 305 Sulfolobus solfata...
2RFB_A    209 d-IIDDALKN---RSGFVEETLRYYSPiqFLPHRFAAEDSYINNKKIKKGDQVIVYLGSANRDETFFDEPDLFKIGRRem 284 Picrophilus torridus
1UE8_A    231 --SWDYVREKg--ALKAVEEALRFSPPv-MRTIRVTKEKVKIRDQVIDEGELVRVWIASANRDEEVFKDPDSFIPDRTpn 305 Sulfolobus tokodaii
1IO8_A    230 --LWQRIREEn-lYLKAIEEALRYSPPv-MRTVRKTKERVKLGDQTIEEGEYVRVWIASANRDEEVFHDGEKFIPDRNpn 305 Sulfolobus solfata...
4YT3_A    264 keVYQELHENldlVPQAVEEMLRFRFNl-IKLDRTVKEDNDLLGVELKEGDSVVVWMSAANMDEEMFEDPFTLNIHRPnn 342 Bacillus megaterium
1F4T_A    230 --LWQRIREEn-lYLKAIEEALRYSPPv-MRTVRKTKERVKLGDQTIEEGEYVRVWIASANRDEEVFHDGEKFIPDRNpn 305 Sulfolobus solfata...
P14762    265 ksLYSELRNNrelAPKAVEEMLRYRFHi-SRRDRTVKQDNELLGVKLKKGDVVIAWMSACNMDETMFENPFSVDIHRPtn 343 Bacillus megateriu...
ACX68487  237 e-VRAAVLGDsqlLPGAIEEVFRYFSPv-QLMFRSLKQDTVLRGQELKQGQFVYIWMASANHDEDVFDQPDVFNIHRNpn 314 Geobacillus sp. Y4...
EEM10559  262 pgVYQELRADfslVPKLIEEVLRFRFP--STNDRKVVQDTKVFGHEMKQGQMVMVWIGAANRDESQFTNAEEFDIHRLen 339 Bacillus pseudomyc...
P27632    262 v-VQQQVRQNtdnVANVIEETLRYYSPv-QAIGRVATEDTELGGVFIKKGSSVISWIASANRDEDKFCKPDCFKIDRPsy 339 Bacillus subtilis ...
Feature 1         ###  ## ###  ##                                             
1IO8_B    306 -phLSFGSGIHLCLGAPLARLEARIAIEEFSKRFRhi-----eiLDTEKVPneVLNGYKRLVVR 363 Sulfolobus solfataricus
2RFB_A    285 --hLAFGIGIHMCLGAPLARLEASIALNDILNHFKrik---idyKKSRLLDnkMVLGYDKLFLS 343 Picrophilus torridus
1UE8_A    306 -phLSFGSGIHLCLGAPLARLEARIALEEFAKKFRvk-----eiVKKEKIDneVLNGYRKLVVR 363 Sulfolobus tokodaii
1IO8_A    306 -phLSFGSGIHLCLGAPLARLEARIAIEEFSKRFRhi-----eiLDTEKVPneVLNGYKRLVVR 363 Sulfolobus solfataricus
4YT3_A    343 kkhLTFGNGPHFCLGAPLARLEAKIALTAFLKKFKhieavpsfqLEENLTDsaTGQTLTSLPLK 406 Bacillus megaterium
1F4T_A    306 -phLSFGSGIHLCLGAPLARLEARIAIEEFSKRFRhi-----eiLDTEKVPneVLNGYKRLVVR 363 Sulfolobus solfataricus
P14762    344 kkhLTFGNGPHFCLGAPLARLEMKIILEAFLEAFShiepfedfeLEPHLTAsaTGQSLTYLPMT 407 Bacillus megaterium NBRC 15308 = A...
ACX68487  315 -phLGLGSGIHYCMGSQLARMETRIALQTLLDRYPqfrr--drsVELARMDstMMFALKELPVI 375 Geobacillus sp. Y412MC10
EEM10559  340 qshLAFGSGPHFCLGAPLARMEARIALTSFVKRFAnirqvegfdVIHHLTDsaMGQALKSLPIV 403 Bacillus pseudomycoides
P27632    340 -phLSFGFGIHFCLGAPLARLEANIALSSLLSMSAciek-aahdEKLEAIPspFVFGVKRLPVR 401 Bacillus subtilis subsp. spizizeni...

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