2X3A,1G12


Conserved Protein Domain Family
M35_peptidyl-Lys_like
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cd11006: M35_peptidyl-Lys_like 
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Peptidase M35 domain of peptidyl-Lys metalloendopeptidases and related proteins
This family M35 Zn2+-metallopeptidase extracellular domain is mostly found in proteins characterized as peptidyl-Lys metalloendopeptidases (MEP; peptidyllysine metalloproteinase; EC 3.4.24.20), including some well-characterized domains in Aeromonas salmonicida subsp. Achromogenes (AsaP1) and Grifola frondosa (GfMEP). These proteins specifically cleave peptidyl-lysine bonds (-X-Lys- where X may even be Pro) in proteins and peptides. AsaP1 peptidase has been shown to be important in the virulence of A. salmonicida subsp. achromogenes, having a major role in the fish innate immune response. Members of this family contain a unique zinc-binding motif (the aspzincin motif), defined by the HExxH + D motif where an aspartic acid is the third zinc ligand and is found in a GTXDXXYG or similar motif C-terminal to the His zinc ligands.
Links
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Statistics
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PSSM-Id: 199912
Aligned: 8 rows
Threshold Bit Score: 185.329
Created: 19-Aug-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active siteZn binding site
Conserved site includes 5 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:The conserved Tyr residue, three residues C-terminal to the zinc-binding Asp, acts as a proton donor during catalysis
  • Structure:1G12: Grifola frondosa M35 Zn peptidase binds zinc
  • Comment:11679721
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
2X3A_A       180 RCTn--------TQKSDLLTALDAASGISNNASSYLAvdk-----pdGQRYRSWFGAYssARWDQAETNFSKIKDAIdn- 245 Aeromonas salmo...
1G12_A         4 GCSs--------SEQSALAAAASAAQSYVAESLSYLQtht-----aaTPRYTTWFGSYisSRHSTVLQHYTDMNSNDf-- 68  Grifola frondosa
YP_262064     44 SCAt-------qADRTAVSNAVAVATTWSTNAYNQVNnneatfrrnqDLAYRTWFGTFsqDRYQRVRAVLDGVRFKLnsg 116 Pseudomonas flu...
ZP_01004462   23 HCSp--------AQDVIVRAALDHAKSLAITAASAVGd---------TPDYARWFGTYsdRNAETARATLKSVVRAIra- 84  Loktanella vest...
ZP_01156479   16 TCEk--------VERDAVLEALAGAEKVVLRAAVSVGd---------TPEYRRWFGPYskENAAVVRAAFKSIHIAIrd- 77  Oceanicola gran...
ZP_01751585   26 GCDv--------EQSRIIDDAFGTAKDLTLKAAVAVGd---------TADYQRWFGDYsaANAEQVRASLKSIVVALrs- 87  Roseobacter sp....
ZP_01909008   79 ACSa--------KEQADARRAVALARSYVDAALGDLErtsvd-eratSPRYTAFFGSYtaERYAEVMDNYRVLDGALte- 148 Plesiocystis pa...
XP_003387075 191 GCGmvtllkgskKERNSVLTVQKRLCSQLKKTRSTISn---------SDLYRVWFGEYneERSNKVREIFQNIIDGLss- 260 Amphimedon quee...

Feature 1                                                                            ##  #       
2X3A_A       246 kPLTFDCs------CKQSy---FAYVYPdq--pYKVYLCkSFWTApv----------tGSDSRAGTIVHQLSHFnvvaGT 304 Aeromonas salmo...
1G12_A        69 sSYSFDCt------CTAAg--tFAYVYPnr--fGTVYLCgAFWKApt----------tGTDSQAGTLVHESSHFtrngGT 128 Grifola frondosa
YP_262064    117 nTLTAYCrvnpndpCDAPgdiaATYVNArg--dVQAWFCtAFFALpp---------rvGFDTQAGTVVHELTHSightTD 185 Pseudomonas flu...
ZP_01004462   85 gGVSLQCdtlrdqgCGNDe---YAWVYAdk--pYHIYLCpSFFDMpvlaslrpgtrasDNGTREGTLIHELSHFrsagGT 159 Loktanella vest...
ZP_01156479   78 aEIKIVCanigeedCDRQm---YANVWShd--hYRINLCpAFFSMprmyafpqgseemENGSREGTIIHEMSHFdvvaRT 152 Oceanicola gran...
ZP_01751585   88 gAVTAQCdlvtdegCDGGn---YAWVYAhe--pYLMHLCpSFFNLppltalrpgdrrsDNGTREGTIVHEISHFrpvaQT 162 Roseobacter sp....
ZP_01909008  149 dPITFDCs------CDLL----ATRSSAvfgesFTFDICpMFSELre---------dtGGFTQAGLLIDGLLQYeavtGM 209 Plesiocystis pa...
XP_003387075 261 hGITYDLng---drCTPAv---PAYTLTnv--pSTVYLCpVFHQFvttmd----ctniGISSKESIILHKLSQVfg-sTQ 327 Amphimedon quee...

Feature 1         #  #                             
2X3A_A       305 DDLGYGQANARNLAktDPVKALNNADNHEYFAEN 338 Aeromonas salmonicida subsp. achromogenes
1G12_A       129 KDYAYGQAAAKSLAtmDPDKAVMNADNHEYFSEN 162 Grifola frondosa
YP_262064    186 IPGAYGVAGSQNLGanRPSDAVENADNYEYYVED 219 Pseudomonas fluorescens Pf-5
ZP_01004462  160 EDHCYTRRDCMGMAlqEPDLAIENADSYQYFTED 193 Loktanella vestfoldensis SKA53
ZP_01156479  153 DDICYSRSACSDRAlrRQEVLIRNADSYQYFAED 186 Oceanicola granulosus HTCC2516
ZP_01751585  163 EDHCYSRSECSQMAqrDARRAIENADSYQYFTED 196 Roseobacter sp. CCS2
ZP_01909008  210 DDQTLDSDEAQELAseDPEAAVRNANGYEHFAQN 243 Plesiocystis pacifica SIR-1
XP_003387075 328 NYNVYGQDNNKELAvtSPEAAVTHSDSYECYYCH 361 Amphimedon queenslandica

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