1X53


Conserved Protein Domain Family
SRPBCC_Aha1

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cd08892: SRPBCC_Aha1 
Click on image for an interactive view with Cn3D
Putative hydrophobic ligand-binding SRPBCC domain of the Hsp90 co-chaperone Aha1 and related proteins
This subfamily includes the C-terminal SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain of Aha1, and related domains. Proteins in this group belong to the SRPBCC domain superfamily of proteins which bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket. Aha1 is one of several co-chaperones, which regulate the dimeric chaperone Hsp90. Hsp90, Aha1, and other accessory proteins interact in a chaperone cycle driven by ATP binding and hydrolysis. Aha1 promotes dimerization of the N-terminal domains of Hsp90, and stimulates its low intrinsic ATPase activity. One Aha1 molecule binds per Hsp90 dimer. The N- and C- terminal domains of Aha1 cooperatively bind across the dimer interface of Hsp90. The C-terminal domain of Aha1 binds the N-terminal Hsp90 ATPase domain. Aha1 may regulate the dwell time of Hsp90 with client proteins. Aha1 may act as either a negative or positive regulator of chaperone-dependent activation, depending on the client protein; for example, it acts as a negative regulator in the case of Saccharomyces cerevisiae MAL63 MAL-activator, and acts as a positive regulator in the case of glucocorticoid receptor and v-Src kinase. The mechanisms by which these opposing functions are achieved are unclear. Aha1 is upregulated in a number of tumor lines co-incident with the activation of several signaling kinases.
Statistics
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PSSM-Id: 176901
View PSSM: cd08892
Aligned: 52 rows
Threshold Bit Score: 133.841
Threshold Setting Gi: 110638500
Created: 30-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 43 residues -Click on image for an interactive view with Cn3D
Feature 1:putative hydrophobic ligand binding site [chemical binding site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1         # # #        ### ##     #        #       # # # # # ## #  #      # ##           
1X53_A        12 KITLKETFLTSPEELYRVFTTq-ELVQAFTHa-PATLEADRGGKFHMVDGNVSGEFTDLVPe-KHIVMKWRFKSWPe-gH 87  human
CAM37997     220 SFSWKMRWGAPVAELYAAMTDp-SRVSVYTRs-PASMDVKAGGLFSFLGGVISGYYVDVQPs-TLIRQQWRLSSWPv-gV 295 Leishmania braz...
EEE24084       4 EICVEELFAVPAHVLYEAFLDphQMMRVGLGa-PAEIDPKVGGRFSWFNKSIEGEITALTPn-KEIQEKWRFAEWEp-mV 80  Toxoplasma gond...
XP_002140882   3 TISSKAYFRVPPSILYQSMLDerELTRLALGs-PCKMDASIGGKFSLYNNYVEGEIEYLEPs-RKIVQKWRCNSWKp-dV 79  Cryptosporidium...
XP_001016619   4 ELKFEVTFEVPSKCIFQALTEq-FEVMKYTRs-PAVVEPKPQGKYSILEGRITGTFLEVDNtkKHIKMTWRMKDWK---S 78  Tetrahymena the...
YP_678709      3 NYKKYYIIPAPPEEVYLALTVp-TTLLLWTGe-EAVMSTEPESEFSLWGGSIEGRNIEFIEg-KKIVQQWYFGDQE---E 76  Cytophaga hutch...
ZP_04777693    3 DFKKYFIIPATPDEVYLALTTe-ITARLWTGd-EASIDPVEGGEFSMWDGAIIGKFISLEPg-KKIVQQWYFGEQE---E 76  Sphingobacteriu...
ZP_02180885    2 DFTLTTQIKATPKQIYKSWLSt-QRHTKMTGs-PAYVSDKVGDIFKAWRGYITGSNIKLEPy-NRIVQSWRSSNFEdseS 78  Flavobacteriale...
YP_592950      4 AIQQSVTFPASARELFETYVDs-RKHTASTGm-PAKISRKVGGKFSGFGGMIGGRNLMLVPg-QMIVQAWRSAAWKktdA 80  Acidobacteria b...
O97256         2 SFEITEEYYVPPEVLFNAFTDa-YTLTRLSRgsLAEVDLKVGGKFSLFSGSILGEFTEITKp-HKIVEKWKFRDWNe-cD 78  Plasmodium falc...
Feature 1          ## # #            # # # #          #########  ###  #      
1X53_A        88 FATITLTFIDk---nGETELCMEGRGIPAp-------EEERTRQGWQRYYFEGIKQTFGY 137 human
CAM37997     296 HSSVVLQLVKee--pGVTTLEFTQSGIPAg-------QLQSVQEGWKANFFEAIKVVFGY 346 Leishmania braziliensis
EEE24084      81 YSDVKMKFDAee--sDTTRLTIEQSGIPLtdkfgngnCDVRVREGWRQHILDRFEKVLGY 138 Toxoplasma gondii GT1
XP_002140882  80 YSKVTIEFLDiigetDCTEIVLKQTSIPSldkfgnpgCSDQCLIGWESNFWDRFEKVLGY 139 Cryptosporidium muris RN66
XP_001016619  79 DSLVNFTFIDr---dDECTLSIVQSDIPNd------vNCENLRKGWMTQIFEPMSSICGY 129 Tetrahymena thermophila
YP_678709     77 PSIVTIILHPdk--qDSTSAELRHTNIPDe-------AYEDMVEGWNGAYFGALQEFYED 127 Cytophaga hutchinsonii ATCC 33406
ZP_04777693   77 PSIVTLKLHEh---kKGTSLEVNHLHIPDe-------AFDDIAEGWTNPYMASLIEFYEE 126 Sphingobacterium spiritivorum ATCC ...
ZP_02180885   79 DSQIEVLLSEn---gEETTLTLNHTNLPE--------SGEHYKEGWEKHYFEPMRQYFEG 127 Flavobacteriales bacterium ALC-1
YP_592950     81 NSILTITFTDt---kSGGRVDLVHVNVPAh-------DHRGVTEGWKKYYWKPWRAYFRK 130 Acidobacteria bacterium Ellin345
O97256        79 YSTVTVEFISvk--eNHTKLKLTHNNIPAsnkyneggVLERCKNGWTQNFLHNIEVILGY 136 Plasmodium falciparum 3D7

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