Conserved Protein Domain Family
RHO_alpha_C_GbcA-like

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cd08884: RHO_alpha_C_GbcA-like 
C-terminal catalytic domain of GbcA (glycine betaine catabolism A) from Pseudomonas aeruginosa PAO1 and related aromatic ring hydroxylating dioxygenases
C-terminal catalytic domain of GbcA (glycine betaine catabolism A) from Pseudomonas aeruginosa PAO1 and related Rieske-type non-heme iron aromatic ring-hydroxylating oxygenases (RHOs, also known as aromatic ring hydroxylating dioxygenases). RHOs utilize non-heme Fe(II) to catalyze the addition of hydroxyl groups to the aromatic ring, an initial step in the oxidative degradation of aromatic compounds. RHOs are composed of either two or three protein components, and are comprised of an electron transport chain (ETC) and an oxygenase. The ETC transfers reducing equivalents from the electron donor to the oxygenase component, which in turn transfers electrons to the oxygen molecules. The oxygenase components are oligomers, either (alpha)n or (alpha)n(beta)n. The alpha subunits are the catalytic components and have an N-terminal domain, which binds a Rieske-like 2Fe-2S cluster, and a C-terminal domain which binds the non-heme Fe(II). The Fe(II) is co-ordinated by conserved His and Asp residues. GbcA is involved in glycine betaine (GB) catabolism in Pseudomonas aeruginosa; it may remove a methyl group from GB via a dioxygenase mechanism, producing dimethylglycine and formaldehyde. This subfamily belongs to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket.
Statistics
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PSSM-Id: 176893
View PSSM: cd08884
Aligned: 33 rows
Threshold Bit Score: 219.835
Threshold Setting Gi: 162451572
Created: 31-Jul-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                   ## ## ##   #                                         
NP_254097    184 EPYDMENAKVAVQTTIREAANWKLVIENNRECYHCnGSHPELLKtLLEWDDVTdprasqafkdqvaactsawdaekipya 263 Pseudomonas aer...
ZP_01913225  178 ASHRLGELRVARRHDYTVAANWKVVIENYGECYHCaVIHPQLHR-LSDFQGGAfveg----------------------- 233 Plesiocystis pa...
NP_926381    172 TGWRLPALRLGARVTYDLRANWKLVFENYNECCHCpPIHPRLAQ-LSPSDSGRn-------------------------- 224 Gloeobacter vio...
YP_477505    188 REWNLSALRRGHRIEYNVQANWKLLFENYSECYHCpLIHPELAR-LSPARSGRn-------------------------- 240 Synechococcus s...
YP_001635947 169 QAWEMARLRRGARITYEVAANWKLIVANYSECYHCpLIHPELVA-ISPWQSGRn-------------------------- 221 Chloroflexus au...
YP_593084    173 RPWRMEELQMVERRVYHLKANWKLVIQNYSECLHCpIVHPLLQK-QSHYMSGDneppq---------------------- 229 Acidobacteria b...
YP_885066    192 SPYRPEDLTIVARHSYELAANWKIIAENYQECYHCsSIHPELSR-ISPPTSGEnlelp---------------------- 248 Mycobacterium s...
ZP_01626035  168 KNYPLSDLRLGHSLEYQVDANWKVILENYNECYHCgPVHPELCKiVPAFRQGGghd------------------------ 223 marine gamma pr...
ZP_05094489  173 QRYPLAELGIGHSISYEVAANWKVICENYNECYHCgPVHPELCEvVPVFKQRGgae------------------------ 228 marine gamma pr...
ZP_04700334  173 RVYELADLECVQAKDYPVASNWKLVAHNVNESLHFpTAHKDLHR-ITDFDDAGtydlk---------------------- 229 Streptomyces al...
Feature 1                                                         ## #       # #      # #        
NP_254097    264 hasfglrnrIVRMPLLDG-------TVSMTMDGKQGSKKLMGriknpdlGSMRILHLPHSWNHCMGDHLIVFTVWPISAQ 336 Pseudomonas aer...
ZP_01913225  234 -----acfnGGPMRLREG-------VQTLSMSGASPLGPIPGlaeadrdQVHYNHVYPNFTLALHPDYVLTHTVWPLSPE 301 Plesiocystis pa...
NP_926381    225 --------dLMEGPFLGGymg-ihpGMALTASGSRSRPPLGTvagedlqRAYYYTLFPNLLFSLHPDYAMVHFLVPLAPD 295 Gloeobacter vio...
YP_477505    241 --------dLMEGPFLGGymllnpdVTSLNMSGQRSLPLISTvrgenqrRAYYYTLMPNLFLSLQPDYVMTHTLWPQGSD 312 Synechococcus s...
YP_001635947 222 --------dLTSGPFLGGyme--ltHESMTLNGQTRRPPLPNlpvedhkRVYYYSIFPNLLLSLHPDYMMAHRLIPRRPD 291 Chloroflexus au...
YP_593084    230 -----ptylGGRMDLRDG-------VKSLTMDGNSIRCALPGlssaderHVYYYCLLPNFFLNLHPDYMLTFTMWPKAAD 297 Acidobacteria b...
YP_885066    249 -----gawmGGWMSIIDG-------AETMSLDGRSGGVAIAGlsehelrTVMYLVGYPNLLVSLHPDYVMTHLMTPLAVD 316 Mycobacterium s...
ZP_01626035  224 ------ldwDIGVPHRDG-------SDTFTASGSTHRAPFPGlsdtektHHKGELFYPNLMLSLAMDHVAAFYVWPKSAG 290 marine gamma pr...
ZP_05094489  229 ------ldwDRGVPHREG-------AVTFTASGTTERRMFPGlnaderiRHKGDLVYPNLFLSASSDHVAVFVLQAKSAN 295 marine gamma pr...
ZP_04700334  230 -----gdlvGAWQSIRGG-------FNSVSMTGRSAREPMALvpeadrrRINWITILPNLLFGFTADYVMMQWVWPESPG 297 Streptomyces al...
Feature 1                              #  ##     ##  #                                         
NP_254097    337 ETLVTTKWLVHKDAv-eGVDYDVARLReVWDATNDQDRRLAEENQRGINSDAYQPGPYSKTYeFGVINFLDWYSERML 413 Pseudomonas aerug...
ZP_01913225  302 QTRVHCEWLVWPAAlegEAPADLSDILeFWDRTNRQDWDMCARVQQGLRSAGYRRGPYTAME-RCIHAFDRWYVEAIG 378 Plesiocystis paci...
NP_926381    296 STRIVCEWYFDPTQi-iQPGFDPTDAVaLWDEINRQDWQVCEWTQQGTASRAYTPGPYAHSE-GLCAAFDREYLKALG 371 Gloeobacter viola...
YP_477505    313 RTRVVCDWLFEPETl-aLDGFDPSEVVeLWDRTNRQDWHLCELTQQGVGSRAYEAGPLSHAE-GLLQAFGREYRRQMQ 388 Synechococcus sp....
YP_001635947 292 ATTIICEWYFDPDVm-aASDFDPSDAVeFWDRTNRQDWHVCELSQQGISSRAYRPGPYAQSE-GLLWQFDQEYLRVMG 367 Chloroflexus aura...
YP_593084    298 QTEIVCEWHFHPDEi-aKPGFNPNDAIeFWDITNKQDWELSDLAQAGISSKGYQPGPYSNRE-ELLLALDRFVLERTQ 373 Acidobacteria bac...
YP_885066    317 RTHVECAWAFPREAa-eRPGFDPAYAVdFWDLTNRQDWAACESVQRGLSSPHARPGPLAPDE-DGVYQFVTRVARAYQ 392 Mycobacterium sme...
ZP_01626035  291 VTQIRCDFLFHPSEl-eKPNFDPMDAVeFWDLVNKQDWKICESVQRGMQNAVFKHGYYAPME-DYSLDIRRYVTDRLD 366 marine gamma prot...
ZP_05094489  296 LTTIDCHFLFESHEm-sKPGFDPSDAVdFWHKVNCQDWAICERVQQGMNSRVHRAGIFSPME-DWNLDIRRYVTDRIG 371 marine gamma prot...
ZP_04700334  298 TCVVRHFWLFHPSEt-aKDDFSHEAVFaLWDKANYEDWEMCERTHRGLSNPQWAPGQLSLDE-EVVSQIDAWVAAETA 373 Streptomyces albu...

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