Conserved Protein Domain Family
RHO_alpha_C_MupW-like

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cd08882: RHO_alpha_C_MupW-like 
C-terminal catalytic domain of Pseudomonas fluorescens MupW and related aromatic ring hydroxylating dioxygenases
C-terminal catalytic domain of the oxygenase alpha subunit of Pseudomonas fluorescens MupW and related Rieske-type non-heme iron aromatic ring-hydroxylating oxygenases (RHOs, also known as aromatic ring hydroxylating dioxygenases). RHOs utilize non-heme Fe(II) to catalyze the addition of hydroxyl groups to the aromatic ring, an initial step in the oxidative degradation of aromatic compounds. RHOs are composed of either two or three protein components, and are comprised of an electron transport chain (ETC) and an oxygenase. The ETC transfers reducing equivalents from the electron donor to the oxygenase component, which in turn transfers electrons to the oxygen molecules. The oxygenase components are oligomers, either (alpha)n or (alpha)n(beta)n. The alpha subunits are the catalytic components and have an N-terminal domain, which binds a Rieske-like 2Fe-2S cluster, and a C-terminal domain which binds the non-heme Fe(II). The Fe(II) is co-ordinated by conserved His and Asp residues. MupW is part of the mupirocin biosynthetic gene cluster in Pseudomonas fluorescens, and may catalyze the oxidation of the 16-methyl group during biosynthesis of this polyketide antibiotic. Mupirocin is a mixture of pseudomonic acids which targets isoleucyl-tRNA synthase and is a strong inhibitor of Gram positive bacterial and mycoplasmal pathogens. This subfamily belongs to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket.
Statistics
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PSSM-Id: 176891
View PSSM: cd08882
Aligned: 36 rows
Threshold Bit Score: 188.315
Threshold Setting Gi: 41410413
Created: 31-Jul-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         ## ## ##   #                                                   
AAM12939     208 AGHSRHILPANWKNVMESFMEGLHAPETHGHTWAHLSDtlq--------ydvRPDERHVSRSfhavgl--svcedHEPLS 277 Pseudomonas flu...
ZP_01225366  186 VMYLSADADFNWKIIRDNFNESYHLPTIHPELSTFINDglp---------dtLFEMYETGHNamwmkghqattrnPEFHT 256 gamma proteobac...
ZP_05093381  191 YVAKTTVAPCNWKVVMDNFNESYHVNTVHKPKGANVEKlrihsgvdtsykttRFDMADEGHGrm----------iMLGGY 260 marine gamma pr...
YP_001165922 186 VLNVSADCDFNWKIIRDNFNESYHLPTIHPELATFINDglp---------ttVFEMYENGHNam----------wMIGHQ 246 Novosphingobium...
ZP_01616962  199 VRHVRSEWGSNWKVGVEAFYESYHLHSVHPETKGVMADlhv---------qyDLYPSGASRMivp-------fgtPSPRL 262 marine gamma pr...
YP_001262758 194 IQHRRSDWGANWKGGVDAFAEIYHLHAVHPQTQCLMDDrt----------qiDLYPGGLSRQfvp-------faqPSPRF 256 Sphingomonas wi...
YP_001165891 188 VAWYRIELNANWKFVTDNFSESYHTRTAHPQVPPWIDQdvd---------saRHEMWPAGHGrtv-------qpmRPSLT 251 Novosphingobium...
ZP_04959178  191 VVWRTLRVNTNWKFASDNFNEAYHIPAVHPQFMPMIDDhys---------ttVFEMYPTGHNrmie------klqPSSRT 255 gamma proteobac...
YP_001165925 194 VVWRTLRVNTNWKFASDNFNEAYHIPAVHPQFEGMIDDhys---------ttVFEMYPTGHNrmie------klqPSSRY 258 Novosphingobium...
ZP_04958956  194 VAWYRVKINANWKFVTDNFSESYHTRTAHPQVPPWIDQdvd---------saRHEMWPKGHGrtv-------qpmHPSIS 257 gamma proteobac...
Feature 1                                                                                        
AAM12939     278 EQAi-------------MDQFHRnvlngksgpyaphlQAGMTARRYmas--------vmRLQYGLASGRDvsGLSDAEav 336 Pseudomonas flu...
ZP_01225366  257 GDVpsp---------ldDVARAWev-----------dPADFNGHTGdirea---iiaakRRLGPERGFTNyeNMSDQQlv 313 gamma proteobac...
ZP_05093381  261 AGPatdkdga-igeplaDILRDWdl-----------dPEDYRGRGEdtrea---lqqarRRLGPGRGYAYfnELNDSQlt 325 marine gamma pr...
YP_001165922 247 ATSrkdyvsgdvppglyEAAEAWgi-----------dPKEYRGRTAdirea---vikakRERGAEAGYDYs-TMTDQQlv 311 Novosphingobium...
ZP_01616962  263 HDQdtvte-----glqmMLQDAGv------------dPANFKGTAKdvrra----iqegKRERSDRLGLEyeRFEDGQls 321 marine gamma pr...
YP_001262758 257 ADQesvnp-----gialMLKDAGi------------dPATYAGTAKetraa----vqaaKRRRAADYGLDydRFSDSQls 315 Sphingomonas wi...
YP_001165891 252 DRPadgte-----hmfaHILRAWdi-----------dPAKYSSYEEfalqgwkdlkqakRRLWRERGYVHyeNMDDEEit 315 Novosphingobium...
ZP_04959178  256 DQKdqvm------plwaEVLSAWgi-----------dPEDYQGKAQegria---lqqarRKLAAERGCHYfdKLTDDElt 315 gamma proteobac...
YP_001165925 259 PDAqqmk------plwaQVLKEWdl-----------dPAEFEGRAQegrla---lqqarRKLGPARGFTHfaALTDDElt 318 Novosphingobium...
ZP_04958956  258 DRPadgrn-----vmfeAVLRQWdi-----------dPDSYDSYEEfafqgwkdlkaakQKLWREKGYVHyeHMNDEEit 321 gamma proteobac...
Feature 1        ## #       # #      # #                                                         
AAM12939     337 DVLQYSLFPNMIFFRgislPPVLRFRPNQNDHNSCIFDLFYLVD--------------IPKGAQRPEpaatvem-----g 397 Pseudomonas flu...
ZP_01225366  314 DYFHCTLFPNLTITMspeqCQILRTEPHPTDPQKCVFHHWVIAP--------------PVKGMTEVEtpigpmpl---ew 376 gamma proteobac...
ZP_05093381  326 DAYHYTLFPNFAVSLwvdgFHFLRARPHPTDPEQCIFDNFWYAP--------------DPDGVTEPVrttaglve---rg 388 marine gamma pr...
YP_001165922 312 DYFHCTLFPNLTITMspeqCQILRTEPHPTDPEKCVFQHWCLYPp-------------NAKMAEVVTpvgpaplr---hd 375 Novosphingobium...
ZP_01616962  322 DSWATGIFPNVQIGChpeaLFFMRFLPHETDPERFYYDTMTMLLp------------vDDPDYSPPGwmglpegidpnkc 389 marine gamma pr...
YP_001262758 316 DSTVYSLFPNAQIGChpeaVFLHRFLPHATDPNQFTYDTMILYRh------------vDAPGYAPPAwmglse------d 377 Sphingomonas wi...
YP_001165891 316 DSPHTVIFPNVTISFlpdnLILFRSEPHATDPEKCYFDLWCMAF--------------PVEGQSEVEsimagvrpl-rev 380 Novosphingobium...
ZP_04959178  316 DQFHHTCFPNLTLTGtpegLHVFRTEPDPEDPNWSTFDYWYLVPqtqgakevatlygmRPYQAAEHEt------------ 383 gamma proteobac...
YP_001165925 319 DQFHHTCFPNLTLTGtpegLHVFRTEPDMEDPNWSTFDYWYLAPe-------------VAGGADVPTly----------- 374 Novosphingobium...
ZP_04958956  322 DSPHTVIFPNVTISFlpdnLVFFRSEPCPDDPEKCYFDLWCMAF--------------PVEGQTEVEsimagpkpl-eev 386 gamma proteobac...
Feature 1                 #  ##               ##  #                                         
AAM12939     398 pddtyEHAGVLPDWl------------GYVYDQDMDNIKNMRAGLQALSndplVLSDLheSRIRHFHNTLMHYLD 460 Pseudomonas fluorescens
ZP_01225366  377 aenrhSVYGDGQSV-------------GYVADQDLSIGTSQQQGLNSRGfsgcILTHQe-KRVQRFHELLNDYVH 437 gamma proteobacteriu...
ZP_05093381  389 teiehEVFSLGEKSm------------GLTIDQDLTIFPGQQRAMSSSGyqgsYLAGQe-SRVSRLHELIDDFIE 450 marine gamma proteob...
YP_001165922 376 aihrhSVYGDGVSL-------------GFVADQDLSIGTTQQQGLNSRGfkgcLLPGQe-KRVQRFHEKLNDMVL 436 Novosphingobium arom...
ZP_01616962  390 erpniEHYSIGEDAg-----------lGLVLSQDSTLLPVVQKGMRSRGfkgqLWGEQe-QRLRHFHVELERRMS 452 marine gamma proteob...
YP_001262758 378 idtsgETRPDIVHTglge-----ppglGEVLDQDSDLLPIVQAGARSRGfrgpLWSEQe-GRLRHFHAELDRRMG 446 Sphingomonas wittich...
YP_001165891 381 aecehRVFDGGRGIpe---------laGQIVYQDMELAENMQAGMHSRGysdaYLSDQe-TRIRFFHEVLNDWIE 445 Novosphingobium arom...
ZP_04959178  384 -----DSFDDYQTSid----------qGDFLTQDLSLAETQQRGLRSMAfqdcYLADQe-SRVRRFHEVLNDYLE 442 gamma proteobacteriu...
YP_001165925 375 ---glRPWKEAEHQtgdftaysaeipqGDFLIQDLDVAVTQQQGLHSLGhddaYLAGQe-TRVRRFHEVINDYIE 445 Novosphingobium arom...
ZP_04958956  387 avcehRDFDGGQGIpe---------laGQIVYQDMSLAEAMQAGMHSKGytdaVLSGQe-TRVRFFHEVLNDYLE 451 gamma proteobacteriu...

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