Conserved Protein Domain Family
RHO_alpha_C_ahdA1c-like

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cd08880: RHO_alpha_C_ahdA1c-like 
C-terminal catalytic domain of the large/alpha subunit (ahdA1c) of a ring-hydroxylating dioxygenase from Sphingomonas sp. strain P2 and related proteins
C-terminal catalytic domain of the large subunit (ahdA1c) of the AhdA3A4A2cA1c salicylate 1-hydroxylase complex from Sphingomonas sp. strain P2, and related Rieske-type non-heme iron aromatic ring-hydroxylating oxygenases (RHOs, also known as aromatic ring hydroxylating dioxygenases). AhdA3A4A2cA1c is one of three known isofunctional salicylate 1-hydroxylase complexes in strain P2, involved in phenanthrene degradation, which catalyze the monooxygenation of salicylate, the metabolite of phenanthene degradation, to produce catechol. This complex prefers salicylate over other substituted salicylates; the other two salicylate 1-hydroxylases have different substrate preferences. RHOs utilize non-heme Fe(II) to catalyze the addition of hydroxyl groups to the aromatic ring, an initial step in the oxidative degradation of aromatic compounds. RHOs are composed of either two or three protein components, and are comprised of an electron transport chain (ETC) and an oxygenase. The ETC transfers reducing equivalents from the electron donor to the oxygenase component, which in turn transfers electrons to the oxygen molecules. The oxygenase components are oligomers, either (alpha)n or (alpha)n(beta)n. The alpha subunits are the catalytic components and have an N-terminal domain, which binds a Rieske-like 2Fe-2S cluster, and a C-terminal domain which binds the non-heme Fe(II). The Fe(II) is co-ordinated by conserved His and Asp residues. Other oxygenases belonging to this subgroup include the alpha subunits of anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1, a polycyclic aromatic hydrocarbon dioxygenase from Cycloclasticus sp. strain A5 (PhnA dioxygenase), salicylate-5-hydroxylase from Ralstonia sp. U2, ortho-halobenzoate 1,2-dioxygenase from Pseudomonas aeruginosa strain JB2, and the terephthalate 1,2-dioxygenase system from Delftia tsuruhatensis strain T7. This subfamily belongs to the SRPBCC (START/RHO_alpha_C/PITP/Bet_v1/CoxG/CalC) domain superfamily of proteins that bind hydrophobic ligands. SRPBCC domains have a deep hydrophobic ligand-binding pocket.
Statistics
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PSSM-Id: 176889
View PSSM: cd08880
Aligned: 22 rows
Threshold Bit Score: 326.908
Threshold Setting Gi: 91780959
Created: 31-Jul-2009
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
Feature 1:putative active site [active site]
Evidence:

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         ## ## ##   #                                                   
BAC65426     193 LGDQRQYVHGNWKLYAENTRDPYHASLLHLFHNtFGLYRSTqtg-kslMDSEKRHSLLYSIaasnddaadkqa-ygdsrt 270 Sphingomonas sp...
AAL17610     179 LGFQRQRIHSNWKLAAENSRDPYHAGLLHLFLNtFGLWRNTsesasiqLDRTGRHCLLATElhtdeeaaeha--sqaqha 256 Pseudomonas aer...
AAO83639     200 LGCTRQYSKSNWKLYMENVKDPYHASMLHLFHTtFNIFRVGmkarsipDANHGLHSIITVTktgddtsaayk--qqnirs 277 Burkholderia ce...
NP_049206    190 LGYARQFMAGNWKLYSENSRDSYHGGLLHLFYPtFGIYRQSqes-aglVSDEGYHTVFTVSkpkgdvdygsfg-deanre 267 Novosphingobium...
BAE93943     123 IGRVAQRIEANWKLYAENSRDSYHGGLLHLFYTtFGLWRPAqdskaeiDEVYGSHNSFQIRkpqqaedaekh--keetar 200 Sphingomonas sp...
YP_002361789 188 LGYHRQYIHGNWKLYLDNTRDPYHASLLHLFHGtFGLIRTSqtgvswvDSEVGWHSIITAEggsddnkldayeneglrty 267 Methylocella si...
YP_717961    194 LGCTRQYSKSNWKLYMENVKDPYHASLLHLFHTtFNIFRVGmkahcvtDDSHGLHSIIMAIkegqdetaggdykeqnirs 273 Sphingomonas sp...
BAC15591     179 IGRFTQKLPNNWKLYFENVKDSYHASLLHMFFTtFELNRLSqkg-gviVDESGGHHVSYSMidrsakddsyk--dqairs 255 Delftia tsuruha...
AAR90187     195 IGRFTQILPNNWKLYVENVKDSYHASILHLFFTtFELNRLSqrg-giiVDESGGHHVSYSAidrdaekdvay--sdqnir 271 Rhodococcus sp....
BAC81540     190 IGHTRQFMKGNWKAYAENSRDSYHGALLHLFYPtFGIYRQGqis-egiTDKVGCHNYFNVSappkedldykdyadeanre 268 Cycloclasticus ...
Feature 1                               ## #       # #      # #                                # 
BAC65426     271 fdtefKLQDMSLLKGrqefddgiTLVILAVFPNLVLQQiqNTLAVRQIVPKGQEAFELVWTHFGYADDDAEMQAIRRKQT 350 Sphingomonas sp...
AAL17610     257 ynpefTLRDSSVVQSqrefddniNLVVLSIFPNLVVHQlgNALSTRHVIPIGEDEFEMVWTQFGFVDDDEELEQLRITHG 336 Pseudomonas aer...
AAO83639     278 fdegfHLEDESILDLvseydedcTNHIQPIFPQLVIQQihNTLVARQILPKGPDNFELIFHFFGYADDTPELRALRIKQA 357 Burkholderia ce...
NP_049206    268 mqgeaKLQDERLLAFrpeiaddvGLHIQSIFPSVVVQQiqNTLATRQIVPHGTDKTELVWTYFGYADDDDETTRHRLRNL 347 Novosphingobium...
BAE93943     201 pldamTLLDPTLLELrqdigddiSLSIQSIFPSVVLQQigQALAVRHMIVREPGAMDLVWTYFGFQDDEEEMTRHRLRAI 280 Sphingomonas sp...
YP_002361789 268 qpdsfKLKDISLLRGrkeypdpvTLLIMSVFPSLVMHQisNTLCFRQVLPKAPDQTELIWHYFGYVDDDAEMQGIRLKQM 347 Methylocella si...
YP_717961    274 ydegfHLEDDSILALveeydivaTNHIQPIFPQLVVQQihNTLVARQLLPKGPNNFELIFHFFGYADDTPELRNLRIKQA 353 Sphingomonas sp...
BAC15591     256 dneryRLKDPSLLEGfeefedgvTLQILSVFPGFVLQQiqNSIAVRQLLPKSISSSELNWTYLGYADDSPEQRKVRLKQA 335 Delftia tsuruha...
AAR90187     272 sesayTLADPTLLEGfsevgddtTLQILSVFPGFVLQQiqNSVAVRQILPTGVERTRLNWTYLGFEEDTPEQREVRLKQA 351 Rhodococcus sp....
BAC81540     269 mqgphQLQDTRVLNFiperndnvALSIQSLFPSVVLQQiqNTLAARQVLPKSTNETELVWTFFGYEDDDAAMDEHRLRNI 348 Cycloclasticus ...
Feature 1         ##     ##  #                                                        
BAC65426     351 NLIGPAGLISMEDGEAVEIVQNAIVGEQqatsFIAMG--GGRAEDADHLVTEGa--IVGFWDSYEKLVG 415 Sphingomonas sp. P2
AAL17610     337 NLIGPSGYVSLEDGDAMERVQRSVSHDKdstsFMGMV--DQNTRSMDYFVTENp--IKGFWQYYQQTLG 401 Pseudomonas aeruginosa
AAO83639     358 NLVGPAGYISMEDTEATELVQRGTVRDAdatsVIEMS--RGNPEQQDTVITESl--IRKFWVGYQKLMG 422 Burkholderia cepacia
NP_049206    348 NLVGPSGLISMEDGEAVELCQQGTIGAEgkrsFVEMG--GDDVRPSYAPMGMDensVRGFWKGYLGLMG 414 Novosphingobium aromaticiv...
BAE93943     281 NMGGPAGYVSLEDGEAVEICTDGIKGPDgh-sLILMDieKAENPTVPMGLDENa--VRGFWKGYLKLMR 346 Sphingomonas sp. A4
YP_002361789 348 NLVGPGGLISMEDGEAIEIVQRATVGEGdgqaVLEMG--GRDAVGADHLVTEGa--IRCMWKGYREIMG 412 Methylocella silvestris BL2
YP_717961    354 NLVGPAGYISMEDTEATELVQQGTVRDGretsVIEMA--KHEPDQSNTLITEKl--IRSFWRGYRKLMK 418 Sphingomonas sp. KA1
BAC15591     336 NLIGPAGFISMEDGAVGGFVQRGIAGAAdldaVIEMG--GDHEGSSEGRATETs--VRGFWKAYRKHMG 400 Delftia tsuruhatensis
AAR90187     352 NLVGPAGYISMEDGCVGGFVQRGIAGAAsqsaVLEMG--GSLAESSESRVTEAs--VRGFWKAYRAAMN 416 Rhodococcus sp. DK17
BAC81540     349 NLVGPAGLISMEDGEAVELVQNGVHRDGelrsFIEMGgnDVEDYTSPMGMDENa--VRGFWKGYRAVMA 415 Cycloclasticus sp. A5

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