2KLO


Conserved Protein Domain Family
Cdt1_c

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cd08767: Cdt1_c 
Click on image for an interactive view with Cn3D
The C-terminal fold of replication licensing factor Cdt1 is essential for Cdt1 activity and directly interacts with MCM2-7 helicase
Cdt1 is a replication licensing factor in eukaryotes that recruits the Minichromosome Maintenance Complex (MCM2-7) to the Origin Recognition Complex (ORC). The Cdt1 protein is divided into three regions based on sequence comparison and biochemical analyses: the N-terminal region (Cdt1_n) binds DNA in a sequence-, strand-, and conformation-independent manner; the middle winged helix fold (Cdt1_m) binds geminin to inhibit both binding of the MCM complex to origins of replication and DNA; and the C-terminal region (Cdt1_c) is essential for Cdt1 activity and directly interacts with the MCM2-7 helicase. Precise duplication of chromosomal DNA is required for genomic stability during replication. Assembly of replication factors to start DNA replication in eukaryotes must occur only once per cell cycle. To form a pre-replicative complex on replication origins in the G phase, ORC first binds origin DNA and triggers the binding of Cdc6 and Cdt1. These two factors recruit a putative replicative helicase and the MCM2-7. The MCM2-7 complex promotes the unwinding of DNA origins, and the binding of additional factors to initiate the DNA replication in S-phase. Cdt1 is present during G1 and early S phase of the cell cycle and is degraded during the late S, G2, and M phases. The winged helix fold structure of Cdt1_m is similar to the structures of Cdt1_c and archaeal homologues of the eukaryotic replication initiator, without apparent sequence similarity.
Statistics
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PSSM-Id: 176572
View PSSM: cd08767
Aligned: 25 rows
Threshold Bit Score: 88.8785
Threshold Setting Gi: 118369817
Created: 4-May-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
putative
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:putative Cdt1-MCM complex binding site
Evidence:
  • Comment:Based on NMR and mutation analyses.
  • Comment:Mutation studies suggest that the binding site for the MCM complex within the Cdt1 C-terminal domain is unlikely to be limited to a confined region.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                         #   #                                       ##   #             
2KLO_A         5 PSALKGVSQALLERIRAKEVQKqlarmtrcpEQELRLQRLERLPELARVLRNVFVs----eRKPALTMEVVCARMVDSCQ 80  house mouse
NP_180685    433 DEILSILPDKLRHAIKEQERKAiedqnpaisLAKRRRKMIACLPKLFNVIHYLIQs----iRRWVITKEELVHKIIAGHS 508 thale cress
NP_191031    352 ISNESGDEKSNYEGDNASDDDSllq---smiERPKTEPEKHNLPQLVNLIHRVFHs----tNRTVITKEELLYKIIANQI 424 thale cress
NP_001040707 520 KEQKSQGAMSLLERIRAKEAIKkaa---eacIDKDLERRKQRLTLLRDRYVRIVCnhytakRAQTMEIDVICKFVQFSSS 596 nematode
CAH65885     453 DELLSFLPKSLLQSVKDKENRAlvekqtgfaDHIKREKLIASLPSIFDIIFLIYQs----rQRTVMTKQELIHKIIASNP 528 Indian rice
XP_001018111 565 KENKTGLSDRLYQLIKAKEEKIneek--lrqSLSKGTEMQRRIPEIKKVVEIIMVyyiqrkVSNMFYASIVDYLTQSSNI 642 Tetrahymena the...
XP_001768172 660 MLMIQGIPSKLVNSVLREELRVaeenrddvvALRRHQQMKAGITLLFDQLRLIVQs----sNKSVFPCKKLLSKLVSSNT 735 Physcomitrella ...
EEH54095     372 GEGAEAIDDADLATVMKREMMArhendpatrAERARRRLYDLLPGLFDAVRSWFAtr---dKKTAPFADIVEQILRTTTK 448 Micromonas pusi...
XP_002409825 291 RECLKGVSQDLLARIRERESFKlvqkmtlrpEHEKERTDLLNLREFIRITRSLFLa----eQKAAIPRDELVCKVKESFS 366 black-legged tick
XP_002741259 616 NTAIKGVPQSLLERIRAKEAAKmeaalirdpAEDKKTDMIARLPELCRILRGFFLa----eKKAAIPIESATSKLVESYK 691 Saccoglossus ko...
Feature 1                #   #   #          # #   #                     #  #
2KLO_A        81 talsPGEMEKHLVLLAELl----pDWLSLHRI-RTDTYVKLDka----vDLAGLTARLA 130 house mouse
NP_180685    509 ditdRKEVEEQLILLQEIv----pEWMSEKKS-SSGDVLVCInk---laSPLTIRSRLE 559 thale cress
NP_191031    425 nitdRREVEEQLSLMLQLv----pDWISETKA-SSGDLLVRInk---msTAETVRARLE 475 thale cress
NP_001040707 597 nsssIPDIMDHLRLLCEVa----pMYISEASL-MNKKYFRFTd-----nNIEAINELLD 645 nematode
CAH65885     529 kivdRGEVEDQLKLLEEIi----pDWISEKTA-RTGDVLCCVdt---amSQAEIRQRLY 579 Indian rice
XP_001018111 643 iiskSSAIEMIDYLIQNCp-----FWITLIPN-KQGSIIRIDks----vALSKILAEIE 691 Tetrahymena thermophila
XP_001768172 736 emtdRTEVEVRLNLLMELa----pEWISAKPSlAGDTLYRIDkn----aDVMGVRKRLC 786 Physcomitrella patens subsp. patens
EEH54095     449 qcasREETERGLRVLAKNaeewcvLWVSEFTG-EELWRVKKVdt----tKVRFVREKLV 502 Micromonas pusilla CCMP1545
XP_002409825 367 cvlaVDEVEALVQLASRVm----pDWFKVLSI-KRGVFVKIVkekdaneLVARITSKLQ 420 black-legged tick
XP_002741259 692 scisVAQMEKHLDVMSEVl----pQWLSIFKI-RKTTYVKIDkk----tDMNTITDKIN 741 Saccoglossus kowalevskii

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