Conserved Protein Domain Family

cd08681: C2_fungal_Inn1p-like 
C2 domain found in fungal Ingression 1 (Inn1) proteins
Saccharomyces cerevisiae Inn1 associates with the contractile actomyosin ring at the end of mitosis and is needed for cytokinesis. The C2 domain of Inn1, located at the N-terminus, is required for ingression of the plasma membrane. The C-terminus is relatively unstructured and contains eight PXXP motifs that are thought to mediate interaction of Inn1 with other proteins with SH3 domains in the cytokinesis proteins Hof1 (an F-BAR protein) and Cyk3 (whose overexpression can restore primary septum formation in Inn1Delta cells) as well as recruiting Inn1 to the bud-neck by binding to Cyk3. Inn1 and Cyk3 appear to cooperate in activating chitin synthase Chs2 for primary septum formation, which allows coordination of actomyosin ring contraction with ingression of the cleavage furrow. It is thought that the C2 domain of Inn1 helps to preserve the link between the actomyosin ring and the plasma membrane, contributing both to membrane ingression, as well as to stability of the contracting ring. Additionally, Inn1 might induce curvature of the plasma membrane adjacent to the contracting ring, thereby promoting ingression of the membrane. It has been shown that the C2 domain of human synaptotagmin induces curvature in target membranes and thereby contributes to fusion of these membranes with synaptic vesicles. The C2 domain was first identified in PKC. C2 domains fold into an 8-standed beta-sandwich that can adopt 2 structural arrangements: Type I and Type II, distinguished by a circular permutation involving their N- and C-terminal beta strands. Many C2 domains are Ca2+-dependent membrane-targeting modules that bind a wide variety of substances including bind phospholipids, inositol polyphosphates, and intracellular proteins. Most C2 domain proteins are either signal transduction enzymes that contain a single C2 domain, such as protein kinase C, or membrane trafficking proteins which contain at least two C2 domains, such as synaptotagmin1. However, there are a few exceptions to this including RIM isoforms and some splice variants of piccolo/aczonin and intersectin which only have a single C2 domain. C2 domains with a calcium binding region have negatively charged residues, primarily aspartates, that serve as ligands for calcium ions.
PSSM-Id: 176063
View PSSM: cd08681
Aligned: 17 rows
Threshold Bit Score: 166.27
Threshold Setting Gi: 164657916
Created: 23-Mar-2010
Updated: 2-Oct-2020
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
Format: Row Display: Color Bits: Type Selection:
P53901        11 GILSVYVSKARDLPnlnkldkQNVMLRLRIahmTRASNTLHragqNPVFHYLEKFDitpe-ikpLMYVEVYCDRrkk-sp 88   baker's yeast
NP_985604     13 GYLEVFVRKAKDLPnmrkmdkQDPFVRLRIahlTEVSGTVFrggqRPVFDFHTVFEltpa-mkhLMYVELFDDQksa--p 89   Ashbya gossypi...
XP_002552040  14 GLLEVYVSRARDLPnlrkldkQSPFVRLRIshmTETSDVAFrggqTPKFDYYAKFDltpd-vkpALAVEVFDDRsp---p 89   Lachancea ther...
XP_451503     11 GRLDVYVSKAKDLPnlrkldkQDPFVKLRVahlTEISPVIYrggqTPKFDYHCVFQltpd-ikpLLCVELYDDHdhkhgs 89   Kluyveromyces ...
EEH50378      30 GTLVVIIDRAKNLPnrktmgkQNPYCAARLgkeAKKTETDMr---GARGQNELRFTvhespdyyQLKVSVFNDDrk---t 103  Paracoccidioid...
XP_502548      6 TATLYRVLTQRNLPnrrtlgkQNPYCIARIaheAKSSPPDIrggqIPKWDHEVRFNlrdnaeqkQLKLTVLDKNdsk--p 83   Yarrowia lipol...
NP_595651      7 GTLVVRIWKAKNLPnkalvgkQSPYCVCRVgevVKRTQTDKrsgqEPSWNAVLEFNipse-syhIMKITVFHEGfrk-hp 84   fission yeast
EEQ43376      27 GTLVVMVIRAKHLPnrrkldkQSPYVVARIgtvAKKTSAAFragqTPEWTHEMRFElsre-rkpILKIDVLDETknd--p 103  Candida albica...
XP_001383332  23 GTLLIHVESAKDLPnrrkldkQNPYVTLRMgttAKKTPSIFrggqRPTWDHDLKFEmsre-rkpILIIDVLDETkne--p 99   Pichia stipiti...
XP_002171558   5 GELVVYVQKARNLDnrqllgkQSPYCVCRIgevVKRTRPNHkggqLPVWDAELRFDikse-aynVMKVAVCCTGfrk-rv 82   Schizosaccharo...
P53901        89 lPIGRCEIDLlnaira-----dpkegyCTWYELkrsgdefaGTIFIELTF 133  baker's yeast
NP_985604     90 rIIGRCEVDLkpalys-----dhedgyDRWYQLslgp-ddaGKIYIELTF 133  Ashbya gossypii ATCC 10895
XP_002552040  90 kLIGQCAVDLtpalyk-----dpedghDDWFPLsfgs-eeaGEIYLELTF 133  Lachancea thermotolerans CBS 6340
XP_451503     90 kLIGKCDVDLlpalls-----dpeeghDRWYHLkrgp-leaGKVYIELTF 133  Kluyveromyces lactis NRRL Y-1140
EEH50378     104 dLIGETTIDLknvvvp------gggqnDLWHGLqckg-kyaGDVRIELTY 146  Paracoccidioides brasiliensis Pb18
XP_502548     84 eLIGDTVLDLqpvfds-----nprdgfDQWHELtykg-kyaGEVYLEMTF 127  Yarrowia lipolytica CLIB122
NP_595651     85 hLIGDTVLSFekamk--------eelqSEWYELknef-qfaGELSVQFKF 125  fission yeast
EEQ43376     104 tPIGNVEIDAsiiftnpennengkyiyDKWYDLtlng-rraGMIYLEMTF 152  Candida albicans WO-1
XP_001383332 100 tPIGHVEIDCstvlhpdn-evngeyflKGWYDMtlne-rraGQILLDFAF 147  Pichia stipitis CBS 6054
XP_002171558  83 lEIGDTVISIkkale--------egefEDWVEIqqny-rfaGQVLIKVQF 123  Schizosaccharomyces japonicus yFS275
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